Degradation and detoxification of endocrine disrupters by white rot fungi and ligninolytic enzymes.

白腐真菌和木质素分解酶对内分泌干扰物的降解和解毒。

• 批准号: 15580144
• 负责人: NISHIDA Tomoaki
• 金额: $ 2.11万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580144/
• 关键词: Endocrine disruptor; White rot fungi; Ligninolytic enzyme; Bisphenol A; Nonylphenol; 17β-Estradiol; Ehinylestradiol; Genistein; マンガンベルオキシダーゼ; ラッカーゼ; エチニルエスチラジオール; エストロゲン様活性

Considerable concern has recently been expressed over the possibility that endocrine disruptors having estrogenic activities may adversely affect reproduction in wildlife and humans. Therefore, endocrine disruptors, bisphenol A (BPA), nonylphenol (NP), 17β-estradiol (E_2), ethinylestradiol (EE_2), and genistein (GE), were treated with white rot fungi under ligninolytic condition with low nitrogen and high-carbon culture medium. Marked decreases in BPA, NP, E_2, and EE_2 were obtained by the treatment and the activities of ligninolytic enzymes, manganese peroxidase (MnP) and laccase, were detected during treatment, thus suggesting that the disappearance of these comopunds is related to ligninolytic enzymes produced extracellularly by white rot fungi. Therefore, BPA, NP, E_2, and EE_2 were treated with MnP and lactase.HPLC analysis demonstrated that all compounds disappeared almost completely in the reaction mixture after 1 h of treatment with MnP or lactase. Using the yeast two-hybrid assay system, it was also confirmed that these enzymatic treatments completely removed the estrogenic activities of BPA, NP, E_2, and EE_2 within a 12-h treatment with MnP or laccase. These results strongly suggest that ligninolytic enzymes are effective in removing the estrogenic activities of BPA, NP, E_2, and EE_2.

最近，人们对具有雌激素活性的内分泌干扰物可能对野生动物和人类的生殖产生不利影响表示了相当大的关注。因此，在低氮高碳条件下，白腐菌对内分泌干扰物双酚A(BPA)、壬基酚(NP)、17β-雌二醇(E_2)、乙烯基雌二醇(EE_2)和染料木素(GE)进行了处理。BPA、NP、E_2和EE_2在处理过程中显著降低，木质素分解酶锰过氧化物酶(MNP)和漆酶活性也显著降低，说明这些复合体的消失与白腐菌胞外产生的木质素分解酶有关。因此，用MNP和乳糖酶处理BPA、NP、E_2和EE_2，用MNP和乳糖酶处理1h后，所有化合物在反应混合物中几乎完全消失。利用酵母双杂交检测系统，也证实了这些酶处理在12h内完全去除了BPA、NP、E_2和EE_2的雌激素活性。这些结果有力地表明，木质素分解酶能有效地去除BPA、NP、E_2和EE_2的雌激素活性。

项目成果

Removal of estrogenic activity of endocrine-disrupting genistein by ligninolytic enzymes from white rot fungi.

• DOI: 10.1016/j.femsle.2005.01.023
• 发表时间: 2005-03
• 期刊: FEMS microbiology letters
• 影响因子: 2.1
• 作者: Yuuki Tamagawa;Hirofumi Hirai;S. Kawai;T. Nishida