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Degradation of Synthetic Polymer by White Rot Fungi

白腐真菌对合成聚合物的降解

基本信息

批准号：
10660161
负责人：
NISHIDA Tomoaki
金额：
$ 2.5万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660161/
关键词：
White rot fungi Polyethylene degradation Manganese peroxidase Lignin degradation Synthetic polymer

项目摘要

Degradation of high-molecular-weight polyethylene membrane by white rot fungi, IZU-154, Phanerochaete chrysosporium, and Trametes versicolor, was investigated under various untritional conditions. IZU-154 showed the most significant polyethylene degradation among the three white rot fungi under nitrogen-or carbon-limited culture conditions. Furthermore, for T.versicolor and P.chrysosporium, the addition of Mn (II) into nitrogen- or carbon-limited culture medium enhanced polyethylene degradation. These results suggest that polyethylene degradation is related to ligninolytic enzyme such as manganese peroxidase (MnP) of white rot fungi. Treatment of polyethylene membrane with partially purified MnP caused significant degradation in the presence of Tween 80, MnSO_4 as Mn(II), and malonate buffer. However, the addition of exogenous H_2O_2 was not necessary for the degradation, although it is recognized that H_2O_2 is essential for the initiation of MnP.A possible role of Tween 80 in the polyethylene degradation by MnP and the basis of the MnP action in the absence of H_2O_2 were investigated. The results showed that the surfactants such as Tween 80 can stabilize MnP in the system. We also indicated that the peroxides derived from the decomposition of malonate by a trace amount of Mn(III) which is formed by autoxidation of Mn (II) can be used for the initiation of MnP.

在不同的营养条件下，研究了白腐菌、IZU-154、黄孢Phanerochaete chrysosporium和花斑曲菌（Trametes versicolor）对高分子量聚乙烯膜的降解。在限氮或限碳培养条件下，三种白腐菌中，IZU-154对聚乙烯的降解效果最显著。此外，对于T.versicolor和P.chrysosporium，在氮或碳限制的培养基中添加Mn （II）可以促进聚乙烯的降解。这些结果表明，聚乙烯的降解与白腐真菌的木质素降解酶如锰过氧化物酶（MnP）有关。用部分纯化的MnP处理聚乙烯膜，在Tween 80、MnSO_4作为Mn（II）和丙二酸盐缓冲液存在下，降解效果显著。然而，外源H_2O_2的加入对MnP的降解并不是必需的，尽管H_2O_2对MnP的起始是必需的。研究了吐温80在MnP降解聚乙烯中的可能作用，以及MnP在缺乏H_2O_2条件下作用的基础。结果表明，Tween 80等表面活性剂能稳定体系中的MnP。我们还指出，由微量Mn（III）自氧化形成的丙二酸盐分解产生的过氧化物可用于引发MnP。