The development of evaluation method of peroxidation product and antioxidant of the food by the photon measurement.
The development of evaluation method of peroxidation product and antioxidant of the food by the photon measurement.
批准号:
15580219
负责人:
SAITO Takahiro
金额:
$2.05万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2003
资助国家:
日本
项目状态:
已结题
起止时间:
2003 至 2004
中文摘要
活性剂是由活性氧和自由基产生的超氧化物,但由于这些生成过程中有许多奢侈的种类和问号,因此被描述为评价有困难。因此,本文以XYZ活性氧清除体系为研究对象,构建该类药剂的评价技术。这是以XYZ系统为前提的:当活性氧(X)和催化物质(Y)、受体(Z)同时存在时,光子释放(XYZ-emission)。在这种情况下,与三种标准试剂(X:2% H_2O_2a,Y:sat)混合。10% CH_3CHO中的C_5H_5COOH(OH)_3,Z:sat。KHCO_3 (10% CH_3CHO)中,xyz的排放量急剧增加,并在10-30秒后达到10^7 cps左右的峰值。之后,它急剧下降,但xyz的排放继续强劲。此外,我们分析了这些xyz发射光谱,波长一直在600 ~ 800 nm之间,最大光谱为678 nm。在氧气、氮气、空调条件下,xyz排放峰值得到认可,但之后xyz排放随氧气量的增加而增加。xyz的峰值与标准X试剂的基数有相关性(相关系数为0.99)。适用于xyz发射积分峰的活性氧种类评价方法。以水稻为对照物的xyz排放峰在不依赖大气的情况下得到了识别和局部最小值。利用该评价方法验证了xyz排放峰与水稻内部元素的关系。水稻xyz释放峰与脂肪酸度指数相关,xyz释放峰与投入相关。脂肪酸度指数是活性氧的产物,该测定方法可以对活性氧进行评价。这些结果证明了利用新型弱发射评价活性氧的技术的充分性。
英文摘要
Agent was hyperoxide out of active oxygen and free radical, but was described as evaluation have difficult, because these generation process have many extravagant kind and question mark. So this paper focus on the XYZ active oxygen scavenging system for constructing of evaluation technique of these agent. This is premised on XYZ system : photon release (XYZ-emission) when active oxygen species (X) and catalytic species (Y), receptors (Z) coexist at the same moment. The following results were obtained : In which case mingle with three standard reagents (X:2% H_2O_2a,Y:sat. C_5H_5COOH(OH)_3 in 10% CH_3CHO,Z:sat. KHCO_3 in 10% CH_3CHO), XYZ-emission increased precipitously, and reach a peak of about 10^7 cps after 10-30 second. Afterward, it decreased precipitously, but XYZ-emission carried over mightily. Additionally, we analyzed these XYZ-emission, wavelength had spectrum of 600-800 nm all the time and maximum spectrum of 678 nm. Peak XYZ-emission get recognition in oxygen and nitrogen gas, air condition, but after XYZ-emission growth in answer to amount of oxygen gas. Peak XYZ-emission had a correlation with cardinal number of standard X reagent (coefficient of correlation : 0.99). Evaluation method of active oxygen species suited to integration peak XYZ-emission. Peak XYZ-emission from rice as reference material got recognition and local minimal value after peak XYZ-emission without recourse to atmosphere. And so we checked up relationship peak XYZ-emission and inside element in rice using this evaluation method. Peak XYZ-emission from rice had a correlation with fat acidity index and peak XYZ-emission had a correlation with input. Fat acidity index was product from active oxygen species, and this measurement method was able to evaluation of active oxygen species. These results demonstrated the adequacy of evaluation technique of active oxygen species using new weak emission.
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