The development of evaluation method of peroxidation product and antioxidant of the food by the photon measurement.

光子测量评价食品过氧化产物和抗氧化剂方法的开发

• 批准号: 15580219
• 负责人: SAITO Takahiro
• 金额: $ 2.05万
• 依托单位: Utsunomiya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580219/
• 关键词: Photon; XYZ system; Chemiluminescence; peroxidation; antioxidant; Food; 機能性; 抗酸化性

Agent was hyperoxide out of active oxygen and free radical, but was described as evaluation have difficult, because these generation process have many extravagant kind and question mark. So this paper focus on the XYZ active oxygen scavenging system for constructing of evaluation technique of these agent. This is premised on XYZ system : photon release (XYZ-emission) when active oxygen species (X) and catalytic species (Y), receptors (Z) coexist at the same moment. The following results were obtained : In which case mingle with three standard reagents (X:2% H_2O_2a,Y:sat. C_5H_5COOH(OH)_3 in 10% CH_3CHO,Z:sat. KHCO_3 in 10% CH_3CHO), XYZ-emission increased precipitously, and reach a peak of about 10^7 cps after 10-30 second. Afterward, it decreased precipitously, but XYZ-emission carried over mightily. Additionally, we analyzed these XYZ-emission, wavelength had spectrum of 600-800 nm all the time and maximum spectrum of 678 nm. Peak XYZ-emission get recognition in oxygen and nitrogen gas, air condition, but after XYZ-emission growth in answer to amount of oxygen gas. Peak XYZ-emission had a correlation with cardinal number of standard X reagent (coefficient of correlation : 0.99). Evaluation method of active oxygen species suited to integration peak XYZ-emission. Peak XYZ-emission from rice as reference material got recognition and local minimal value after peak XYZ-emission without recourse to atmosphere. And so we checked up relationship peak XYZ-emission and inside element in rice using this evaluation method. Peak XYZ-emission from rice had a correlation with fat acidity index and peak XYZ-emission had a correlation with input. Fat acidity index was product from active oxygen species, and this measurement method was able to evaluation of active oxygen species. These results demonstrated the adequacy of evaluation technique of active oxygen species using new weak emission.

氧化剂是由过氧化产物中的活性氧和自由基产生的，但被描述为评价有困难，因为这些生成过程有许多奢侈的种类和问号。因此，本文以XYZ活性氧清除系统为研究对象，构建了这类药剂的评价技术。这是以XYZ体系为前提的：当活性氧物种(X)和催化物种(Y)、受体(Z)同时共存时的光子释放(XYZ-发射)。在此情况下，用三种标准试剂(X：2%H_2O_2a，Y：Sat)混合。C_5H_5COOH(OH)_3在10%CH3CHO中，Z：Sat。KHCO3在10%CH3CHO中)，XYZ-发射急剧增加，并在10-30秒后达到约10^7 cps的峰值。之后，它急剧下降，但XYZ-发射仍然很强。此外，我们对这些XYZ发射进行了分析，波长始终在600-800 nm之间，最大光谱为678 nm。XYZ-发射峰值在氧气和氮气、空气条件下得到识别，但在XYZ-发射之后随着氧气气体量的增加而增加。XYZ发射峰与标准X试剂基数呈正相关(r=0.99)。适合积分峰XYZ发射的活性氧物种评价方法。在不依赖大气的情况下，水稻的XYZ峰值发射得到了识别，并在峰值后出现了局部极小值。因此，我们利用该评价方法对水稻XYZ辐射峰值与体内元素的关系进行了检验。水稻XYZ-排放峰值与脂肪酸度指数相关，XYZ-排放峰值与投入相关。脂肪酸度指数是由活性氧物种产生的，该测定方法能够对活性氧物种进行评价。这些结果证明了利用新的弱发射来评价活性氧物种的技术的充分性。