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Search of new regulating factors and its regulatory mechanism for proliferation and differentiation in ruminant adipocytes.

反刍动物脂肪细胞增殖分化新调控因子及其调控机制的寻找

基本信息

批准号：
15580246
负责人：
SASAKI Shin-ichi
金额：
$ 2.24万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580246/
关键词：
Ruminant Adipocytes Proliferation and Differentiation Co-culture Nuclear Transcription Factor Leptin TNF-α Adipogenin GPCR43 三次元コラーゲンゲルマトリックス培養 VFA CLA インスリン IGF-I

项目摘要

Summary of this research results is listed below.1.We have established a co-culture system with ovine mature adipocytes and preadipocytes and the data from their experiments indicate that mature adipocytes regulate proliferation and differentiation of preadipocytes.2.Ovine adipocytes actively synthesis and secrete many members of the cytoldne family, such as leputin and TNF-α. They act as inhibitory factor in proliferation and differentiation of mature adipocytes and preadipocytes, and the synthesis and secretion of them are regulated by insulin and IGF-I.3.Our studies demonstrated that the activation of MAP kinase and PI3-kinase contributes to the proliferation and differentiation process of ovine preadipocytes, respectively, and the activation of PI3-kinase may be necessary in the activation of a nuclear transcription factor, PPAr-γ.4.Next, we have searched out new genes related to adipogenesis.(1)Adipogenin, a new adipocyte-specific gene, have been identified Since the expression of adipogenin mRNA is up-regulated during adipocyte differentiation, it is indicated that adipogenin may be involved with adipogenesis, as one of the regulatory of the adipose tissue development.(2)GPCR43, G protein-coupled receptor family having short chain fatty acids as their ligand, mRNA is up-regulated during preadipocyte differentiation. The stimulatory effect of propionate in differentiation of preadipocytes due to anti-lipolytic action through GPCR43.

主要研究结果如下：1.建立了绵羊成熟脂肪细胞与前体脂肪细胞共培养体系，实验结果表明成熟脂肪细胞对前体脂肪细胞的增殖和分化具有调节作用; 2.绵羊脂肪细胞能够主动合成和分泌细胞因子家族的多种成员，如leputin和TNF-α。它们在成熟脂肪细胞和前脂肪细胞的增殖和分化过程中起抑制作用，其合成和分泌受胰岛素和IGF-I的调节。3.本研究证实MAP激酶和PI 3-激酶的激活分别参与了绵羊前脂肪细胞的增殖和分化过程。PI 3-激酶的激活可能是核转录因子PPAr-γ激活所必需的。（1）Adipogenin是一个新的脂肪细胞特异性基因，在脂肪细胞分化过程中Adipogenin mRNA表达上调，提示Adipogenin可能参与脂肪细胞的形成，是脂肪组织发育的调控因子之一。（2）GPCR 43是一个以短链脂肪酸为配体的G蛋白偶联受体家族，其mRNA在前脂肪细胞分化过程中上调。丙酸盐通过GPCR的抗脂肪分解作用在前脂肪细胞分化中的刺激作用43.