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Research for Bio-prevention and decreasing of influence for estrogenic chemical matter by using of specific plants

利用特定植物进行生物预防和减少雌激素化学物质影响的研究

基本信息

批准号：
15580298
负责人：
MURAMOTO Shigeki
金额：
$ 2.24万
依托单位：
Kibi International University (2004-2005)Okayama University (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

To examine the living thing influence and the decrease plan of the environmental hormones external material in the waters. Himedaka was tried for Atrazine and Benomyl, and the exposure experiment was conducted by using (Orazis latipes), Bidorogenin density(VTG) in blood was assumed to be an index and the evaluation an examination of the estrogen action of the environmental hormone external material were tried. The VTG density in 0-100 μg/L of Atrazine tended to rise gradually, and the influence as the environmental hormone was guessed. It was admitted that the underwater Atrazine density was decreased when a specific plant was grown to these at the same time, the VTG density decreased in the blood of the medaka, too and the effect of the phytoremediation was clear. By the way, it was confirmed that the density decreased to 80-145μg/mL in the blood of a female medaka of the contrast district in the plant cultivation processing district compared with VTG density about 330 μg/mL, and the hormone external action had been controlled. A similar tendency was confirmed bout Benomyl though it was not more remarkable than Atrazine. The pollution waters with environmental hormone external material were assumed deprivation was calculated provisionally from the exposure experiment by the plant, and the zoning of the water purification was examined. The pollution water with the environmental hormone external material were assumed, deprivation was calculated provisionally from the exposure experiment by the plant and the zoning of the waters purification was examined. In the trial calculation of the amount of deprivation of Benomyl in the waters by a specific plant, it was guessed that it was Kosihikari,red Camitikara, Fuji Article 2 barley for each 1ha, and it had the deprivation ability of about 13-17kg/ha. It was similar and 1.8-2 in the trial calculation of Atrazine Strengh of the influence on the root was shown by the deprivation ability of about 1/10 of kg/ha and Benomyl.

探讨水体中环境激素外源物质对生物的影响及减少计划。对Himedaka进行了阿特拉津和苯甲酰的测定，并用（Orazis latipes）进行了暴露实验，假设血中Bidorogenin密度（VTG）为指标，并尝试对环境激素外源性物质的雌激素作用进行了评价和检查。在0 ~ 100 μg/L阿特拉津作用下，VTG密度呈逐渐升高的趋势，并推测其受环境激素的影响。结果表明，当特定植物生长到这些区域时，水中阿特拉津密度降低，同时medaka血液中VTG密度也降低，植物修复效果明显。通过这种方法，证实了植物栽培加工区对照区雌性medaka血液中的浓度从VTG浓度约330 μg/mL降至80-145μg/mL，激素的外作用得到了控制。苯甲酰也有类似的倾向，但并不比阿特拉津更显著。假定含环境激素外源物质的污染水体由工厂暴露试验临时计算剥夺量，并对水质净化的分区进行了考察。假设含环境激素外源物质的污染水体，从植物的暴露实验中临时计算了剥夺量，并对水体净化的分区进行了考察。在试验计算某一特定植物对水体中苯甲酰的剥夺量时，推测其为每1ha的小光、红Camitikara、富士2号大麦，其剥夺能力约为13-17kg/ha。在阿特拉津的试验计算中，其对根的影响强度与1.8-2相似，约为1/10 kg/ha和苯甲酰的剥夺能力。