Development of Novel Separation System for Analysis of Physiological Function utilizing Environmentally-responsive polymers

利用环境响应性聚合物开发用于生理功能分析的新型分离系统

• 批准号: 15590049
• 负责人: KANAZAWA Hideko
• 金额: $ 2.24万
• 依托单位: Kyoritsu University of Pharmacy
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590049/
• 关键词: poly (N-isopropylacrylamide); temperature-responsive chromatography; functional polymer; functional surface; HPLC; packing material; environmentally responsive; ポリ(N-イソプロピルアクリルアミド

We report development of a novel analytical HPLC technique of nucleotides with a constant aqueous mobile phase. In this study, we have designed a new pH/temperature-responsive copolymer of N-isopropylacrylamide (NIPAAm), butyl methacrylate (BMA) and N,N-dimethylaminopropylacrylamide (DMAPAAm). The copolymer was grafted on aminopropyl silica beads and the products were evaluated as HPLC packing materials for an ion-exchange temperature responsive chromatography. The property of the surface of the stationary phase altered from hydrophilic to hydrophobic and from charged to non-charged by changes in temperature and pH. In addition, it is possible that appears and hides ion-exchange groups on the polymer chain surface by the temperature changes. Nucleotides interactions with this stationary phase were controlled by the temperature and the pH with constant aqueous mobile phase. PH-/temperature responsive chromatography is expected to be useful for separation of pharmaceuticals and biomolecules as well as nucleotides. We believe that the proposed temperature-responsive chromatography is likely to be useful for diverse applications in biotechnology including protein purification and bioseparations.

我们报告了一种新的分析核苷酸的HPLC技术与恒定的水移动的相的发展。本研究设计了一种新型的pH/温度响应性N-异丙基丙烯酰胺（NIPAAm）、甲基丙烯酸丁酯（BMA）和N，N-二甲基氨基丙基丙烯酰胺（DMAPAAm）共聚物。将共聚物接枝到氨丙基硅胶珠上，并将产物作为离子交换温度响应色谱的HPLC填料进行评价。温度和pH值的变化使固定相表面的性质从亲水性变为疏水性，从带电变为不带电。此外，温度变化可能使聚合物链表面的离子交换基团出现和隐藏。核苷酸与该固定相的相互作用受温度和pH控制，水移动的相恒定。pH-/温度响应色谱有望用于药物和生物分子以及核苷酸的分离。我们相信，建议的温度响应色谱可能是有用的生物技术，包括蛋白质纯化和生物分离的各种应用。

项目成果

Hideko Kanazawa: "Temperature-responsive polymers for liquid phase separations"Analytical and Bioanalytical Chemistry. 378. 46-48 (2004)

Hideko Kanazawa：“用于液相分离的温度响应聚合物”分析和生物分析化学。

温度応答性クロマトグラフィー

温度响应色谱

• 发表时间: 2004
• 期刊: ぶんせき 6
• 作者: E.Ayano;Y.Okada;C.Sakamoto;H.Kanazawa;T.Okano;M.Ando;T.Nishimura;Hideko Kanazawa;金澤 秀子
• 通讯作者: 金澤 秀子

Temperature- and pH-responsive aminopropyl-silica ion-exchange columns grafted with copolymers of N-isopropylacrylamide.

• DOI: 10.1016/j.chroma.2003.09.010
• 发表时间: 2004-03
• 期刊: Journal of chromatography. A
• 作者: Chikako Sakamoto;Y. Okada;H. Kanazawa;E. Ayano;T. Nishimura;M. Ando;A. Kikuchi;T. Okano

Analysis of herbicides in water using temperature-responsive chromatography and an aqueous mobile phase

• DOI: 10.1016/j.chroma.2005.01.100
• 发表时间: 2005-04-01
• 期刊: JOURNAL OF CHROMATOGRAPHY A
• 影响因子: 4.1
• 作者: Ayaro, E;Okada, Y;Nishimura, T
• 通讯作者: Nishimura, T

金澤秀子: "機能性高分子を用いた温度応答性クロマトグラフィーによるタンパク分離"Hitachi Scientific Instrument News. 46. 7-11 (2004)

Hideko Kanazawa：“使用功能性聚合物通过温度响应色谱法分离蛋白质”日立科学仪器新闻 46. 7-11 (2004)。