Development of environment-friendly separation materials using high functional surface

高功能表面环保型分离材料的开发

• 批准号: 11680845
• 负责人: KANAZAWA Hideko
• 金额: $ 1.92万
• 依托单位: Kyoritsu University of Pharmacy
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680845/
• 关键词: poly (N-isopropylacrylamide); HPLC stationary phase; functional polymers; Temperature-responsive chromatography; environment-friendly; 充填剤; 環境応答性; ポリ(N-イソプロピルアクリルアミド; 温度応答性クロストグラフィー; ビスフェノール A; 充てん剤

In this study, a new concept of chromatography, environmentally responsive chromatography, is proposed. We have been studied new method of HPLC using packing materials modified with the stimuli responsive polymers, poly (N-isopropylacrylamide) (PNIPAAm) and its copolymer. PNIPAAm has the sharpest phase transition of the class of thermosensitive N-alkyl acrylamide polymers. This feature makes it the most suitable for studies and practical applications. We designed and synthesized novel temperature-responsive polymers with additional sites responsive to other environmental stimuli such as changes in pH. They showed hydrophilic-hydrophobic phase transitions in response to changes of temperature as PNIPAAm but the transition temperatures were affected by changes in pH. Stimuli-responsive polymers, which change their structure and physical properties in response to external signals, comprise a new set of materials with interesting applications in biomaterial science and technology. Novel ch … More romatography system may be achieved using stimuli responsive polymers, which alter their structure and physical properties in response to external environmental change. These methods are expected to be applicable for the separation of pharmaceuticals, nucleic acid, peptides and proteins. The newly developed pH/temperature-responsive stationary phases are useful in method development as an extra tool to optimize selectivity by adjusting both of the temperature and pH rather than changing the mobile phase composition. As both of temperature and pH gradients are possible in this system, the combination of these two gradient modes is promising as an alternative to solvent gradient elution. These results show that the column modified with poly (NIPAAm-co-BMA-co-DMAPAAm) could respond to both temperature and pH to modulate retention of charged compounds using aqueous mobile phase. The ability of the proposed environmentally-responsive polymer modified stationary phase to separate the solutes without the use of organic solvent is advantageous from the point of view of keeping biological activity, environmental reasons, and economical cost of mobile phases. Less

在这项研究中，提出了色谱的新概念——环境响应色谱。我们研究了使用刺激响应聚合物聚（N-异丙基丙烯酰胺）（PNIPAAm）及其共聚物改性的填充材料进行高效液相色谱的新方法。 PNIPAAm 在热敏 N-烷基丙烯酰胺聚合物中具有最急剧的相变。这一特性使其最适合研究和实际应用。我们设计并合成了新型温度响应型聚合物，具有响应其他环境刺激（例如 pH 值变化）的附加位点。它们表现出与 PNIPAAm 一样响应温度变化的亲水-疏水相变，但转变温度受到 pH 值变化的影响。刺激响应聚合物可响应外部信号而改变其结构和物理性质，由一组新材料组成，在生物材料科学和技术中具有有趣的应用。新型色谱系统可以使用刺激响应聚合物来实现，该聚合物可以响应外部环境变化而改变其结构和物理特性。这些方法有望适用于药物、核酸、肽和蛋白质的分离。新开发的 pH/温度响应固定相可作为一种额外工具用于方法开发，通过调整温度和 pH 值而不是改变流动相组成来优化选择性。由于该系统中可以同时存在温度和 pH 梯度，因此这两种梯度模式的组合有望作为溶剂梯度洗脱的替代方案。这些结果表明，用聚 (NIPAAm-co-BMA-co-DMAPAAm) 改性的色谱柱可以响应温度和 pH 值，从而使用水性流动相调节带电化合物的保留。从保持生物活性、环境原因和流动相经济成本的角度来看，所提出的环境响应性聚合物改性固定相能够在不使用有机溶剂的情况下分离溶质的能力是有利的。较少的

项目成果

K.Yamamoto,H.Kanazawa,Y.Matsushima,N.Takai,A.Kikuchi,T.Okano: "Preparation of Packing Materials for Temperature-Responsive Chromatography.Modification of Silica Surface with Poly(N-isopropylacrylamide)"Chromaotgraphy. 21. 209-215 (2000)

K.Yamamoto，H.Kanazawa，Y.Matsushima，N.Takai，A.Kikuchi，T.Okano：“温度响应色谱填料的制备。用聚（N-异丙基丙烯酰胺）对二氧化硅表面进行改性”色谱法。

H.Kanazawa,Y.Matsushima,T.Okano: "「Advances in Chromatography vol.41」 ed.by P.R.Brown and E.Grushka."Marcel Dekker. 311-336 (2001)

H.Kanazawa、Y.Matsushima、T.Okano：“Advances in Chromatography vol.41”，P.R.Brown 和 E.Grushka 编辑。”Marcel Dekker. 311-336 (2001)

Hideko Kanazawa: "Temperature responsive chromatography "Advances in Chromatography vol.41"ed.by P.R.Brown and E.Grushka (Chapter 8)"Marcel Dekker, Inc. (USA). 311-336 (2001)

Hideko Kanazawa：“温度响应色谱法“Advances in Chromatography vol.41”ed.by P.R.Brown 和 E.Grushka（第 8 章）”Marcel Dekker, Inc.（美国）。

E. Ayano, H. Kanazawa, A. Kikuchi, T. Okano: "Environmental responsive chromatography"Anal. Sci.. 17. 873-874 (2001)

E. Ayano、H. Kanazawa、A. Kikuchi、T. Okano：“环境响应色谱”分析。

H. Kanazawa, E. Ayano, K. Chiba, A. Kikuchi, T. Okano: "Temperature Responsive chromatography -Effects of temperature gradient-"Anal. Sci.. 17. 875-876 (2001)

H. Kanazawa、E. Ayano、K. Chiba、A. Kikuchi、T. Okano：“温度响应色谱-温度梯度的影响-”Anal。