权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Histochemical studies on glycolipids especially from functional and developmental aspects

糖脂的组织化学研究，特别是功能和发育方面的研究

基本信息

批准号：
15590173
负责人：
KAWAKAMI Hayato
金额：
$ 2.3万
依托单位：
Kyorin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590173/
关键词：
glucolipid ganglioside globoside monoclonal antibody small intestine renal corpuscle diabetes histochemistry 神経叢

项目摘要

Histochemical localizations of various kinds of glycolipids in rat kidney and small intestine were investigated with the use of confocal laser scanning microscopy and transmission electron microscopy. Samples were isolated from normal Wistar rats and diabetic Goto-Kakizaki(GK)rats and were treated with mouse monoclonal antibodies(mAbs) against globoside(Gb3) or gangliosides(GM1, GD2, GQ1b, GD3, GD1a, GM3, o-acetyl-GD3) followed by treatment with Cy3-labeled secondary antibody for confocal microscopy and HRP-labeled antibody for electron microscopy, respectively. In the case of small intestine, accumulations of some gangliosides (GD2, GQ1b etc.) were observed around the nerve cell body in the myenteric plexus and also along the plasma membranes of inner-circular smooth muscle cells. Intestinal gland epithelial cells, especially their basolateral plasma membranes were positive for globoside (Gb3). In normal kidney cortex, each antibody revealed specific distribution pattern especially in glomeruli and in urinary tubular epithelial cells. In GK rats, anti-Gb3 and anti-GD1a antibodies demonstrated increased stainability for glomeruli compared with those in normal Wistar rats. Electron microscopical observation revealed that, the binding sites of anti-Gb3 in GK rats were glomerular basement membranes and the plasma membranes of podocytes and endothelial cells. Specific distributions of glycolipids in rat kidney cortex and their changes following diabetic disorder may provide some important clues for understanding renal functions especially in glomerular area.

应用共聚焦激光扫描显微镜和透射电镜对大鼠肾脏和小肠中各种糖脂的组织化学定位进行了研究。从正常Wistar大鼠和糖尿病Goto-Kakizaki（GK）大鼠中分离样品，并用小鼠单克隆抗体（mAb）处理针对globoside（Gb 3）或神经节苷脂（GM 1、GD 2、GQ 1b、GD 3、GD 1a、GM 3、o-乙酰基-GD 3），然后分别用Cy 3标记的第二抗体用于共聚焦显微镜检查和HRP标记的抗体用于电子显微镜检查。在小肠的情况下，一些神经节苷脂（GD 2，GQ 1b等）的积累。在肌间神经丛中的神经细胞体周围以及沿内环平滑肌细胞的质膜沿着。肠腺上皮细胞，尤其是其基底侧质膜的球蛋白苷（Gb 3）阳性。在正常肾皮质中，各抗体均表现出特异性分布，尤其是在肾小球和肾小管上皮细胞中。在GK大鼠，抗Gb 3和抗GD 1a抗体表现出增加的肾小球滤过率与正常Wistar大鼠相比。电镜观察显示，GK大鼠抗Gb 3抗体的结合部位为肾小球基底膜、足细胞和内皮细胞的质膜。糖脂在大鼠肾皮质的分布及其在糖尿病后的变化可能为了解肾功能特别是肾小球区的功能提供重要线索。