Cytochemical studies on the interaction between cultured endothelial cells and platelets

培养内皮细胞与血小板相互作用的细胞化学研究

• 批准号: 07670032
• 负责人: KAWAKAMI Hayato
• 金额: $ 1.41万
• 依托单位: Kyorin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670032/
• 关键词: platelet; endothelium; carbohydrate; tumor cells; metastasis; lectin; cell adhesion; tunicamycin

Mouse colon carcinoma (colon 26) cells were shown to attach to cultured hepatic sinusoidal endothelial cells only in the presence of PRP (platelet-rich-plasma). Most of tumor cells firstly attached to the endothelial cells not directly but via aggregated platelets. In addition, almost all of the tumor cells that were attached to the endothelial cells showed positive staining with ECA (Erythrina crystagalli agglutinin), whereas nearly half of the cells before adhesion were negative for ECA binding. ECA-positive cells, when separated from ECA-negative ones, revealed strong potency for metastasis to the liver compared with ECA-negative ones. ECA-positive materials were extracted from the crude membrane fraction of colon 26 cells and were found to suppress the platelet adhesion. These results indicate that the ECA-positive sugar chains on the surface of colon 26 cells should mediate the binding between colon 26 cells and platelets and the attachment of tumor cells to the endothelium. The attachment of colon carcinoma cells to hepatic sinusoidal cells induced as above may cause the succsessive events in establishing cancer metastasis.

小鼠结肠癌（colon 26）细胞仅在PRP（富血小板血浆）存在下才能附着在培养的肝窦内皮细胞上。大多数肿瘤细胞最初不是直接粘附在内皮细胞上，而是通过聚集的血小板粘附在内皮细胞上。此外，几乎所有附着到内皮细胞上的肿瘤细胞都显示出ECA（刺桐凝集素）阳性染色，而粘附前近一半的细胞对ECA结合呈阴性。与ECA阴性细胞相比，ECA阳性细胞在与ECA阴性细胞分离时显示出向肝脏转移的强大潜能。从结肠26细胞的粗膜部分中提取ECA阳性物质，发现其抑制血小板粘附。这些结果表明，结肠26细胞表面上的ECA阳性糖链应介导结肠26细胞与血小板之间的结合以及肿瘤细胞与内皮的附着。上述诱导的结肠癌细胞与肝窦细胞的粘附可能导致建立癌转移的连续事件。

项目成果

Kudo A, Zhou X, Kawakami H, Hirano H: "Changes of immunoreactive androgen receptor in mouse testicular germ cells during seminiferous epithelial cycle" Acta Histochem Cytochem. 29 (Suppl.). 483-484 (1996)

Kudo A、Zhou X、Kawakami H、Hirano H：“生精上皮周期期间小鼠睾丸生殖细胞中免疫反应性雄激素受体的变化”Acta Histochem Cytochem。

Miyata H, Hori T, Kawakami H, Hirano H: Hippocampal pathology of experimetal status epileptcus assessed by means of lectin histochemistry. The Hippocampus : Functions and Clinical Relevace, (Kato N, ed). Elsevier Science B.V., 229-231 (1996)

Miyata H、Hori T、Kawakami H、Hirano H：通过凝集素组织化学评估实验性癫痫持续状态的海马病理学。

川上速人: "電子顕微鏡でみるミクロの世界-生物編「血小板」" 学際企画, 2 (1995)

川上隼人：“通过电子显微镜看到的微观世界 - 生物学版‘血小板’”跨学科项目，2 (1995)

Yamaguchi H, Osaki T, Taguchi H, Yamamoto T, Hanawa T, Fukuda M, Kawakami H, Hirano H, Kamiya S.: "Growth inhibition of Helicobacter pylori by monoclonal antibody to heat-shock protein 60"Microbiol Immunol.. 41. 909-916 (1997)

Yamaguchi H、Osaki T、Taguchi H、Yamamoto T、Hanawa T、Fukuda M、Kawakami H、Hirano H、Kamiya S.：“热休克蛋白 60 的单克隆抗体对幽门螺杆菌的生长抑制”微生物免疫学 41。

Kawakami H, Kanai Y, Yamori T, Tsuruo T, Hirano H: "Histochemical detection of sugar residues by joint use of specific antibodies and lectins" Acta Histochem cytochem.28. 191-192 (1995)

Kawakami H、Kanai Y、Yamori T、Tsuruo T、Hirano H：“通过联合使用特异性抗体和凝集素对糖残基进行组织化学检测”Acta Histochem cytochem.28。