Microbes and polysaccharide recognition of Langerin
朗格林的微生物和多糖识别
基本信息
- 批准号:15590336
- 负责人:
- 金额:$ 2.05万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Langerin (CD207) is a type II C-type lectin that is expressed in epidermal dendritic cells (Langerhans cells ; LC). In this project, we performed functional analyses as an antigen and microbe uptake receptor of CD207 in comparison with other type II C-type lectins in mouse DC-SIGN family, such as SIGNR1, SIGNR3 and mDC-SIGN.1)Internalization of tagged-CD207 molecule by cross-linking with anti-tag antibody was observed by cytofluorometer and fluorescent microscope. In comparison with the lectins in DC-SIGN family, CD207 showed the strongest internalizing activity comparable with SIGNR3. Internalized antibody via CD207 was sorted into intracellular vesicle distinct from CD71^+ early endosome and CD107^+ late endosome, suggesting that antigens captured by CD207 are processed differently from those via MMR and CD205.2)CD207-transfectants captured dextran and OVA. These activities were inhibited by GlcNAc and Fucose as well as mannan. So CD207 is a typical mannose/mannan type lectin.3)The transfectants recognized zymosan and heat-killed C.albicans, in a mannan-dependent manner. Unlike SIGNR1, CD207 was incapable of recognizing Gram-negative bacteria, such as E.coli and S.typhimurium.4)Internalization of the yeast particles via CD207 was confirmed by a quenching method of extracellular particles using acidic trypan blue.5)CD207 was shown to physically associate with TLR2, which formed heterodimer with TLR1 or TLR6 to mediate activation signal by various substances from microbes.Collectively, our results indicate that CD207 possibly play a role as an antigen receptor to capture and process polysaccha-rides, glycoproteins and microbes. In addition, CD207 might be involved in TLR2-dependent cellular responses against pathogen.
Langerin(CD207)是一种表达于表皮树突状细胞(Langerhans cell,LC)的II型C型凝集素。在本项目中,我们对CD207作为抗原和微生物摄取受体的功能进行了分析,并与小鼠DC-SIGN家族中的其他II型C型凝集素,如SIGNR1、SIGNR3和MDC-SIGN1)进行了比较。1)通过细胞荧光仪和荧光显微镜观察了标记的CD207分子与抗Tag抗体交联后内化的情况。与DC-SIGN家族的凝集素相比,CD207具有与SIGNR3相当的最强的内化活性。通过CD207内化的抗体被分选到细胞内的囊泡中,这与CD71~+早期的内体和CD107~+的晚期内体不同,这表明CD207捕获的抗原的处理方式与通过MMR和CD205.2的不同。这些活性被GlcNAc、岩藻糖和甘露聚糖抑制。因此,CD207是一种典型的甘露糖/甘露聚糖型凝集素。3)转基因细胞以甘露聚糖依赖的方式识别酵母多糖和热灭活白念珠菌。与SIGNR1不同的是,CD207不能识别革兰氏阴性菌,如大肠杆菌和鼠伤寒沙门氏菌。4)CD207通过胞外颗粒的酸性台盼蓝猝灭方法证实了酵母颗粒的内化。5)CD207与TLR2形成异源二聚体,与TLR1或TLR6形成异二聚体,介导来自微生物的各种物质的激活信号。此外,CD207可能参与了依赖TLR2的细胞对病原体的反应。
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Association of SIGNR1 with TLR4/MD-2 enhances signal transduction by recognition of LPS in Gram-negative bacteria. (^* the authors contributed equally to this work).
SIGNR1 与 TLR4/MD-2 的结合可通过识别革兰氏阴性细菌中的 LPS 来增强信号转导。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Nagaoka;K.^*;Takahara;K.^*;Tanaka;K.;Yoshida;H.;Steinman;R.M.;Saitoh;S.;Akashi-Takamura;S.;Miyake;K.;Kang;Y.S.;Park;C.G.;Inaba;K.
- 通讯作者:K.
Initial functional comparison of the mDC-SIGN, SIGNR1,SIGNR3 and Langerin, mouse C-type lectin
mDC-SIGN、SIGNR1、SIGNR3 和 Langerin(小鼠 C 型凝集素)的初步功能比较
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Takahara;K.
- 通讯作者:K.
Takahara, K.: "Initial functional comparison of the mDC-SIGN, SIGNR1, SIGNR3 and Langerin, mouse C-type lectin"Int.Immunol.. (in press). (2004)
Takahara, K.:“mDC-SIGN、SIGNR1、SIGNR3 和 Langerin、小鼠 C 型凝集素的初始功能比较”Int.Immunol..(出版中)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Wang, W.-M.: "Transforming growth factor-β induces secretion of activated ADAMTS-2 : A procollagen III N-proteinase"J.Biol.Chem. 278・21. 19549-19557 (2003)
Wang, W.-M.:“转化生长因子-β 诱导激活的 ADAMTS-2 的分泌:前胶原 III N-蛋白酶”J.Biol.Chem. 278·21 (2003)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Defective development of splenic and epidermal CD4+ dendritic cells in mice deficient for IFN regulatory factor-2
- DOI:10.1073/pnas.0400610101
- 发表时间:2004-03-16
- 期刊:
- 影响因子:11.1
- 作者:Ichikawa, E;Hida, S;Taki, S
- 通讯作者:Taki, S
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TAKAHARA Kazuhiko其他文献
Amelioration of DSS-induced colitis in DCIR1-deficient mice
改善 DCIR1 缺陷小鼠中 DSS 诱导的结肠炎
- DOI:
- 发表时间:
2016 - 期刊:
- 影响因子:0
- 作者:
TAKAHARA Kazuhiko;IWAKURA Yoichiro;INABA Kayo - 通讯作者:
INABA Kayo
臨床免疫・アレルギー科(C型レクチンによる生体応答制御-恒常性の維持と応用-)
临床免疫学/过敏(C型凝集素控制生物反应 - 体内平衡维持和应用)
- DOI:
- 发表时间:
2016 - 期刊:
- 影响因子:0
- 作者:
TAKAHARA Kazuhiko;IWAKURA Yoichiro;INABA Kayo;高原 和彦 - 通讯作者:
高原 和彦
TAKAHARA Kazuhiko的其他文献
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{{ truncateString('TAKAHARA Kazuhiko', 18)}}的其他基金
Observation of in vivo dynamics of macrophage subsets based on expression of lectin molecule
基于凝集素分子表达的巨噬细胞亚群体内动态观察
- 批准号:
21590417 - 财政年份:2009
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Functional analyses of mouse lectin SIGNR family
小鼠凝集素SIGNR家族的功能分析
- 批准号:
19590389 - 财政年份:2007
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Microbe recognition and effect on TLR response by C-type lection expressing in immune cells.
免疫细胞中表达的 C 型分泌物对微生物的识别及其对 TLR 反应的影响。
- 批准号:
17590342 - 财政年份:2005
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Behavior and function of mouse Langerin^+ cells in skin and mucosa.
小鼠皮肤和粘膜中 Langerin^ 细胞的行为和功能。
- 批准号:
13670213 - 财政年份:2001
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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针对朗格汉斯细胞和皮肤微生物群治疗严重特应性皮炎的建议
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