A mutant of Escherichia coli enterotoxin inducing a specific Tb1-type of T cells to varicella-zoster vaccine enhances the production of IL-12 by IFNγ-stimulated macrophages

大肠杆菌肠毒素突变体诱导特定 Tb1 型 T 细胞接种水痘带状疱疹疫苗，增强 IFNγ 刺激的巨噬细胞产生 IL-12

• 批准号: 15590403
• 负责人: TSUJI Takao
• 金额: $ 2.3万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590403/
• 关键词: mucosal adjuvant; eat-labile enterotoxin; cellular immunity; mutant; Th1; Th2 balance; macrophage; IL-12; TNF α; Th1; 毒素原性大腸菌; 易熱性下痢毒素; 水痘ワクチン; IL-10

A mutant of Escherchia colt enterotoxin induces specific Th1-type T cells to varicella-zoster vaccine. The mutant increased IL-12p40, TNFα and nitric oxide production by IFNγ -stimulated bone marrow macrophages but cholera toxin did not. Anti-TNFα antibodies blocked its stimulation of IL-12p40 production but iNOS inhibitor did not. IL-12p40 and IL-12p35 production was stimulated at the level of mRNA formation by the mutant. Cholera toxin suppressed IL-12(31 expression by spleen T cells stimulated with anti-CD3 antibodies but the mutant did not. These findings indicate that the mutant may induce Th 1-type response to the vaccine through its IL-12 and TNFα induction by macrophages.

一种突变型土拨鼠肠毒素诱导水痘-带状疱疹疫苗特异性Th 1型T细胞该突变体可增加IFNγ刺激的骨髓巨噬细胞产生IL-12 p40、TNFα和一氧化氮，但对霍乱毒素无影响。抗TNF α抗体可阻断其对IL-12 p40的刺激作用，而iNOS抑制剂则无此作用。IL-12 p40和IL-12 p35的产生在mRNA形成水平上被突变体刺激。霍乱毒素抑制了用抗CD 3抗体刺激的脾T细胞的IL-12 β 1表达，但突变体没有。这些结果表明，突变体可能通过巨噬细胞诱导IL-12和TNFα诱导对疫苗的Th 1型应答。

项目成果

Clostridium perfringens alpha-toxin-induced hemolysis of horse erythrocytes is dependent on Ca2+ uptake

• DOI: 10.1016/s0005-2736(03)00140-8
• 发表时间: 2003-06-27
• 期刊: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
• 影响因子: 3.4
• 作者: Ochi, S;Oda, M;Sakurai, J
• 通讯作者: Sakurai, J

Induction of thymus-derived γδ T cells by Escherichia coli enterotoxin B-subunit in peritoneal cavity of mice.

大肠杆菌肠毒素 B 亚基在小鼠腹腔内诱导胸腺来源的 γδ T 细胞。

• 发表时间: 2005
• 期刊: Clinical and Diagnostic Laboratory Immunology 12
• 作者: Shimizu T;Sasaki K;Tsuji T. et al.
• 通讯作者: Tsuji T. et al.

組換え豚丹毒菌防御抗原の回収精製方法

一种重组猪丹毒保护性抗原的采集和纯化方法

• 发表时间: 2000

Induction of thymus-derived γδ T cells by Escherichia coil enterotoxin B-subunit in peritoneal cavity of mice.

大肠杆菌肠毒素 B 亚基在小鼠腹腔内诱导胸腺来源的 γδ T 细胞。

• 发表时间: 2005
• 期刊: Clinical and Diagnostic Laboratory Immunology 12
• 作者: Shimizu T;Sasaki K;Tsuji T.et al.
• 通讯作者: Tsuji T.et al.

Live varicella vaccine polarizes the mucosal adjuvant action of CT or CT-B on specific Th1-type helper T cells with a single nasal coadministration

通过单次鼻腔联合给药，水痘活疫苗可极化 CT 或 CT-B 对特定 Th1 型辅助 T 细胞的粘膜佐剂作用

• 发表时间: 2003
• 期刊: J.Med.Virol. 70
• 作者: Sasaki K;Tsuji T. et al.
• 通讯作者: Tsuji T. et al.