A mutant of Escherichia coli enterotoxin inducing a specific Tb1-type of T cells to varicella-zoster vaccine enhances the production of IL-12 by IFNγ-stimulated macrophages

大肠杆菌肠毒素突变体诱导特定 Tb1 型 T 细胞接种水痘带状疱疹疫苗，增强 IFNγ 刺激的巨噬细胞产生 IL-12

• 批准号: 15590403
• 负责人: TSUJI Takao
• 金额: $ 2.3万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590403/
• 关键词: mucosal adjuvant; eat-labile enterotoxin; cellular immunity; mutant; Th1; Th2 balance; macrophage; IL-12; TNF α; Th1; 毒素原性大腸菌; 易熱性下痢毒素; 水痘ワクチン; IL-10

A mutant of Escherchia colt enterotoxin induces specific Th1-type T cells to varicella-zoster vaccine. The mutant increased IL-12p40, TNFα and nitric oxide production by IFNγ -stimulated bone marrow macrophages but cholera toxin did not. Anti-TNFα antibodies blocked its stimulation of IL-12p40 production but iNOS inhibitor did not. IL-12p40 and IL-12p35 production was stimulated at the level of mRNA formation by the mutant. Cholera toxin suppressed IL-12(31 expression by spleen T cells stimulated with anti-CD3 antibodies but the mutant did not. These findings indicate that the mutant may induce Th 1-type response to the vaccine through its IL-12 and TNFα induction by macrophages.

大埃雪氏菌肠毒素突变体可诱导特定的Th1型T细胞到水痘带状疱疹疫苗。突变体增加了IFNγ刺激的骨髓巨噬细胞的IL -12P40，TNFα和一氧化氮的产生，但霍乱毒素没有增加。抗TNFα抗体阻止了其对IL-12P40产生的刺激，但iNOS抑制剂却没有。突变体在mRNA形成水平上刺激了IL-12P40和IL-12P35的产生。霍乱毒素抑制了IL-12（31用抗CD3抗体刺激的Sleen T细胞表达，但突变体没有刺激。这些发现表明，突变体可能通过其IL-12和TNFα诱导诱导巨噬细胞对疫苗诱导1型疫苗的反应。

项目成果

Induction of thymus-derived γδ T cells by Escherichia coli enterotoxin B-subunit in peritoneal cavity of mice.

大肠杆菌肠毒素 B 亚基在小鼠腹腔内诱导胸腺来源的 γδ T 细胞。

• 发表时间: 2005
• 期刊: Clinical and Diagnostic Laboratory Immunology 12
• 作者: Shimizu T;Sasaki K;Tsuji T. et al.
• 通讯作者: Tsuji T. et al.

組換え豚丹毒菌防御抗原の回収精製方法

一种重组猪丹毒保护性抗原的采集和纯化方法

• 发表时间: 2000

Induction of thymus-derived γδ T cells by Escherichia coli enterotoxin B-subunit in peritonea] cavity of mice.

小鼠腹腔内大肠杆菌肠毒素 B 亚基诱导胸腺来源的 γδ T 细胞。

• 发表时间: 2005
• 期刊: Clinical and Diagnostic Laboratory Immunology
• 作者: Shimizu T;Sasaki K;Tsuji T. et al.
• 通讯作者: Tsuji T. et al.

Sasaki, Tsuji et al.: "Escherichia coli enterotoxin B subunit reduces CD5+ B cell numbersin the peritoneal cavity in mice and stimulates polyclonal IgM productiondependent on its binding to GM1-ganglioside"Curr.Trend Immunol.. (In press). (2004)

Sasaki、Tsuji 等人：“大肠杆菌肠毒素 B 亚基可减少小鼠腹腔内的 CD5 B 细胞数量，并刺激多克隆 IgM 的产生，这取决于其与 GM1-神经节苷脂的结合”Curr.Trend Nutritionol..（正在出版）。

A mutant of E, coli enterotoxin inducing a specific Th1-type of T cells to VZV vaccine enhances the production of IL-12 by IFNγ- stimulated MΦ

大肠杆菌肠毒素突变体诱导特定 Th1 型 T 细胞接种 VZV 疫苗，通过 IFNγ 刺激的 MΦ 增强 IL-12 的产生

• 发表时间: 2005
• 期刊: Vaccine (in press)
• 作者: Shimizu T;Sasaki K;Tsuji T. et al.
• 通讯作者: Tsuji T. et al.