Molecular principles of quorum sensing-mediated phage defense in Vibrio cholerae

霍乱弧菌群体感应介导的噬菌体防御的分子原理

• 批准号: 464459808
• 负责人: Professor Dr. Kai Papenfort
• 金额: --
• 依托单位: Professur für Mikrobielle Genregulation
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/464459808?language=en
• 关键词: Molecular; principles; quorum; sensing; mediated

The field of phage biology is awash with examples of how fundamental science can drive conceptual advances in molecular biology, genetics and microbiology with a strong impact on biotechnology and biomedicine. This is also true for virulent microorganisms such as the major human pathogen Vibrio cholerae, whose pathogenic potential and epidemic proliferation largely depends on the interaction with environmental phages. This project aims to characterize the interaction of V. cholerae with lytic and lysogenic phages, as well as role of intercellular communication (a.k.a. quorum sensing) in this process. Our preliminary data show that quorum sensing can protect V. cholerae from lytic phages, whereas certain lysogenic phages repurpose quorum sensing signals to control their lytic lifecycle. To better understand the molecular principles underlying these processes, we will combine biochemical, genetic, and microscopy approaches to pinpoint the factors that drive phage-microbe interaction with a strong emphasis on the molecular mechanisms underlying these processes. Specifically, we will harness the power of synthetic regulatory RNAs to expose new mechanisms of phage defense. At the most general level, the proposed work will provide insights into phage-microbe interactions, intercellular communication, and the molecular principles underlying phage resistance. At a more specific level, the research will advance our understanding of phage replication processes and how phage infection is perceived and defended by the bacterial host. At a practical level, our research could expose new strategies for controlling phage infections, with possible applications in biotechnology and the food industry.

噬菌体生物学领域充斥着基础科学如何推动分子生物学，遗传学和微生物学概念进步的例子，对生物技术和生物医学产生了强烈的影响。对于人类主要病原体霍乱弧菌等有毒微生物来说也是如此，其致病潜力和流行扩散在很大程度上取决于与环境噬菌体的相互作用。该项目旨在表征霍乱弧菌与溶解性和溶原性弧菌的相互作用，以及细胞间通讯（a.k.a.在这一过程中，？我们的初步数据表明，群体感应可以保护霍乱弧菌免受溶解性毒素的侵害，而某些溶原性毒素会改变群体感应信号的用途，以控制其溶解性生命周期。为了更好地理解这些过程背后的分子原理，我们将结合联合收割机生物化学，遗传学和显微镜的方法，以确定驱动噬菌体-微生物相互作用的因素，重点是这些过程背后的分子机制。具体来说，我们将利用合成调控RNA的力量来揭示噬菌体防御的新机制。在最一般的水平上，拟议的工作将提供深入了解噬菌体-微生物相互作用，细胞间通讯，以及噬菌体抗性的分子原理。在更具体的层面上，这项研究将促进我们对噬菌体复制过程以及噬菌体感染如何被细菌宿主感知和防御的理解。在实践层面上，我们的研究可以揭示控制噬菌体感染的新策略，并可能应用于生物技术和食品工业。