Identification of novel leukemia associated antigen which may useful for immunotherapy of leukemia patients

新型白血病相关抗原的鉴定可用于白血病患者的免疫治疗

• 批准号: 15590997
• 负责人: FURUKAWA Tatsuo
• 金额: $ 2.24万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590997/
• 关键词: leukemia associated antigens; CTL; SEREX; 細胞傷害活性; Leukemia; 白血病特異抗原; HLA

1.Serological identification of antigens by the recombinant expression cloning (SEREX) method has successfully identified many tumor antigens that elicit humoral immune response. In the present study, we defined a novel antigen that elicited humoral immune response in patients with leukemia and that was overexpressed in leukemia cells. Apg-1 can elicit humoral immune responses in leukemia patients and might be potential novel targets for antigen-specific immunotherapy for leukemia.2.Using in vitro transcripted mRNA electroporation gene delivery, we developed CTL line activated by the antigen loading DC. However, we could not detect any cytotoxic activity in this activated T cells for leukemic cell line.3.Thereafter, we identified four 9-mer peptides with HLA-A24-binding motifs encoded by Apg-1 using computer calculation. Of these, one peptide developed efficiently effector cells which lysed leukemic cell line expressing both Apg-1 and HLA-A24.Our results indicate that Apg-1 antigen may be one of leukemia associated antigens.

1.通过重组表达克隆（SEREX）方法的抗原血清学鉴定已经成功地鉴定了许多引起体液免疫应答的肿瘤抗原。在本研究中，我们定义了一种新的抗原，引起白血病患者的体液免疫反应，并在白血病细胞中过表达。Apg-1可诱导白血病患者产生体液免疫应答，有望成为白血病抗原特异性免疫治疗的新靶点。2.采用体外转录mRNA电穿孔基因转染技术，建立了负载抗原的DC激活的CTL细胞株。然而，我们没有检测到这种活化的T细胞对白血病细胞系的任何细胞毒活性。3.随后，我们通过计算机计算鉴定了Apg-1编码的四个具有HLA-A24结合基序的9聚体肽。其中一种多肽能有效地杀伤同时表达Apg-1和HLA-A_（24）的白血病细胞系。我们的结果提示Apg-1抗原可能是白血病相关抗原之一。

项目成果

Cytoplasmic expression of EGFP in dendritic cells transfected with in vitro transcribed mRNA or cellular total RNA extracted from EGFP expressing leukemia cells

• DOI: 10.1385/mo:20:4:335
• 发表时间: 2003
• 期刊: Medical Oncology
• 影响因子: 3.4
• 作者: Masuhiro Takahashi;M. Narita;F. Ayres;Naoko Satoh;T. Abe;Toshio Yanao;T. Furukawa;K. Toba;T. Hirohashi;Y. Aizawa

The effects of STI571 on antigen presentation of dendritic cells generated from patients with chronic myelogenous leukemia

STI571对慢性粒细胞白血病患者产生的树突状细胞抗原呈递的影响

• 发表时间: 2003
• 期刊: Hematol Oncol 21
• 作者: Sato N

Human dendritic cells mediate anti-tumor activity against hematopoietic tumor cells without direct contact and Fas/FasL killing pathway.

人树突状细胞介导针对造血肿瘤细胞的抗肿瘤活性，无需直接接触和Fas/FasL杀伤途径。

• 发表时间: 2004
• 期刊: Oncol Rep 11
• 作者: 広松雄治;Sato N;広松雄治;Ayres FM
• 通讯作者: Ayres FM

High-dose cytosine arabinoside and etoposide with total body irradiation as a preparatory regimen for allogeneic hematopoietic stem-cell transplantation in patients with acute lymphoblastic leukemia.

大剂量阿糖胞苷和依托泊苷全身照射作为急性淋巴细胞白血病患者异基因造血干细胞移植的准备方案。

• 发表时间: 2004
• 期刊: Bone Marrow Transplant 34
• 作者: Sato N