A comprehensive study of the regulatory mechanisms for prognosis-related leukaemia cell surface antigen expression

预后相关白血病细胞表面抗原表达调控机制的综合研究

基本信息

项目摘要

Using a CD7-expressing human acute myeloblasticleukaemia (AML) cell line, KG-1a, and its parentalline lacking CD7 expression, KG-1, we examined the CD7 gene structure and the levels in mRNA and protein expression, by cytogenetic observation, genomic PCR-direct sequencing, quantitative RT-PCR and flowcytometry. These analyses revealed that CD7 expression in KG-1a cells is 260-times higher than in KG -1 cells and is regulated at the mRNA level. To address further the regulatory mechanisms of CD7 expression, analyses on the transcription frequency or the mRNA stability are needed.
本研究采用细胞遗传学观察、基因组PCR直接测序、定量RT-PCR和流式细胞术等方法,对表达CD 7的人急性成髓细胞白血病(AML)细胞系KG-1a及其亲本细胞系KG-1的CD 7基因结构、mRNA和蛋白表达水平进行了研究。这些分析表明,KG-1a细胞中的CD 7表达比KG-1细胞高260倍,并且在mRNA水平上受到调节。为了进一步阐明CD 7表达的调控机制,需要对转录频率或mRNA稳定性进行分析。

项目成果

期刊论文数量(19)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Suminoe A et al.: "mRNA expression of apoptosis-associated genes in infant acute lymphoblastic leukemia : low Fas expression is an independent predictor for poor prognosis"Leukemia. 18. 365-368 (2004)
Suminoe A 等人:“婴儿急性淋巴细胞白血病中凋亡相关基因的 mRNA 表达:低 Fas 表达是不良预后的独立预测因子”白血病。
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    0
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Takagi M et al.: "Identification and characterization of polymorphic variations of the ataxia telangiectasia mutated (ATM) gene in childhood Hodgkin disease"Blood. 103. 283-290 (2004)
Takagi M 等人:“儿童霍奇金病中共济失调毛细血管扩张突变 (ATM) 基因多态性变异的鉴定和表征”血液。
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    0
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Prognostic factors for relapsed childhood acute lymphoblastic leukemia: Impact of allogeneic stem cell transplantation-a report from the Kyushu-Yamaguchi Children's Cancer Study Group.
复发性儿童急性淋巴细胞白血病的预后因素:同种异体干细胞移植的影响——九州山口儿童癌症研究组的报告。
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Matsuzaki;A. et al.
  • 通讯作者:
    A. et al.
Allogeneic stem-cell transplantation with reduced conditioning intensity as a novel immunotherapy and antiviral therapy for adult T-cell leukemia/lymphoma
  • DOI:
    10.1182/blood-2004-11-4193
  • 发表时间:
    2005-05-15
  • 期刊:
  • 影响因子:
    20.3
  • 作者:
    Okamura, J;Utsunomiya, A;Saburi, Y
  • 通讯作者:
    Saburi, Y
Two modes of microsatellite instability in human cancer: differential connection of defective DNA mismatch repair to dinucleotide repeat instability.
人类癌症中微卫星不稳定性的两种模式:有缺陷的DNA不匹配修复与二核苷酸重复不稳定性的差异连接。
  • DOI:
    10.1093/nar/gki303
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    14.9
  • 作者:
    Oda, S;Maehara, Y;Ikeda, Y;Oki, E;Egashira, A;Okamura, Y;Takahashi, I;Kakeji, Y;Sumiyoshi, Y;Miyashita, K;Yamada, Y;Zhao, Y;Hattori, H;Taguchi, K;Ikeuchi, T;Tsuzuki, T;Sekiguchi, M;Karran, P;Yoshida, MA
  • 通讯作者:
    Yoshida, MA
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OKAMURA Jun其他文献

OKAMURA Jun的其他文献

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{{ truncateString('OKAMURA Jun', 18)}}的其他基金

DNA mismatch repair deficiency in paediatric haematological malignancies: research and application for chemotherapeutic strategies
儿科血液恶性肿瘤DNA错配修复缺陷:化疗策略的研究与应用
  • 批准号:
    22591173
  • 财政年份:
    2010
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular mechanisms of the antineoplastic agents inhibiting DNA replication and their applications to cancer patient treatmen
抗肿瘤药物抑制DNA复制的分子机制及其在癌症患者治疗中的应用
  • 批准号:
    19591274
  • 财政年份:
    2007
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Identification of biomarkers to predict anticancer drug sensitivities by means of transgene experiments
通过转基因实验鉴定生物标志物以预测抗癌药物敏感性
  • 批准号:
    17591136
  • 财政年份:
    2005
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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Transcriptional assessment of haematopoietic differentiation to risk-stratify acute lymphoblastic leukaemia
造血分化的转录评估对急性淋巴细胞白血病的风险分层
  • 批准号:
    MR/Y009568/1
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    2024
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Combining two unique AI platforms for the discovery of novel genetic therapeutic targets & preclinical validation of synthetic biomolecules to treat Acute myeloid leukaemia (AML).
结合两个独特的人工智能平台来发现新的基因治疗靶点
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    10090332
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    2024
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    Collaborative R&D
KAT2A PROTACs targetting the differentiation of blasts and leukemic stem cells for the treatment of Acute Myeloid Leukaemia
KAT2A PROTAC 靶向原始细胞和白血病干细胞的分化,用于治疗急性髓系白血病
  • 批准号:
    MR/X029557/1
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    2024
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FITEAML: Functional Interrogation of Transposable Elements in Acute Myeloid Leukaemia
FITEAML:急性髓系白血病转座元件的功能研究
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Development of a national framework for the investigation and management of familial leukaemia
制定国家家族性白血病调查和管理框架
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    MR/V037242/1
  • 财政年份:
    2023
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Harnessing infection-associated modifiers of paediatric acute lymphoblastic leukaemia progression for therapeutic benefit
利用感染相关的小儿急性淋巴细胞白血病进展调节因子获得治疗益处
  • 批准号:
    494943
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    2023
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Identifying Clinically Relevant Metabolite and Lipid Markers for Precise Diagnosis and Treatment of CNS-Leukaemia
识别临床相关代谢物和脂质标志物以精确诊断和治疗中枢神经系统白血病
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    2888061
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    Studentship
ICF: Chimeric antigen receptor T cells targeting CD123, CD33 and CLL1 for therapy of Acute Myeloid Leukaemia
ICF:靶向 CD123、CD33 和 CLL1 的嵌合抗原受体 T 细胞用于治疗急性髓系白血病
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    MR/X03030X/1
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    2023
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    $ 2.24万
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The implication of chromosomal somatic structural variants in the clinical course of chronic lymphocytic leukaemia
染色体体细胞结构变异在慢性淋巴细胞白血病临床过程中的意义
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    23K14635
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    Grant-in-Aid for Early-Career Scientists
Chronic Lymphocytic Leukaemia: uncovering immunity during first-line treatment
慢性淋巴细胞白血病:一线治疗期间揭示免疫力
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    MR/X019306/1
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