Genome-wide Association Study of Psoriasis Using Polymorphic Microsatellite Markers

银屑病的全基因组研究近年来取得了显著进展。这些研究已经确定了几个染色体上的许多银屑病易感位点。然而，银屑病的易感基因尚未确定。在这项研究中，我们系统地进行了银屑病的关联分析，以确定所有银屑病易感位点。本研究调查的对象为无血缘关系的日本寻常型牛皮癣患者。独立的125个样本被合并为第一、第二和第三组。同样，独立的125名不相关的健康个体作为对照，分为第一组、第二组和第三组。我们使用第一组汇集的DNA进行第一次筛选。之后，第二组和第三组用于第二次和第三次筛选，以避免假阳性关联。结果，推测的牛皮癣候选基因座被缩小到不到40个区域。

Genome-wide studies of psoriasis has made remarkable progress recently. These studies have identified many psoriasis susceptibility loci on several chromosomes. However, psoriasis susceptibility genes have not identified. In this study, we have systematically performed association analysis of psoriasis to define all psoriasis susceptibility loci. The subjects investigated in this study were unrelated Japanese patients with psoriasis vulgaris. Independent 125 samples were pooled for the 1st, 2nd and 3rd sets. Similarly, independent 125 unrelated healthy individuals as controls were pooled for the 1st, 2nd and 3rd sets. We used the 1st set pooled DNA for the 1st screening. After that, 2nd and 3rd sets were used for the 2nd and 3rd screening to avoid false positive associations. As the result, putative psoriasis candidate loci were narrowed down to less than 40 regions.