权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Investigation for function of vasopressin V1b and α1-adrenoceptors in the central nervous system under stress condition

应激条件下中枢神经系统加压素V1b和α1-肾上腺素受体的功能研究

基本信息

批准号：
15591233
负责人：
ISHIZUKA Yuta
金额：
$ 1.86万
依托单位：
University of Miyazaki (2004)宮崎医科大学 (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Three α1-adrenoceptor(AR) subtypes have been identified by molecular cloning. Several studies have analyzed the functional roles of α1-ARs in the central nervous system. Recent data suggest that the α1-ARs are involved in locomotion, cognitive functions and in the control of motor activity. However, very little is known about the functional role of distinct α1-AR subtypes in the brain. In order to clarify the role of the α1-AR subtypes for learning behavior, knockout mice deficient of the α1b-AR and α1bd-AR were used. Studies were performed with male wild-type(WT) mice and knockout(KO) mice deficient for the α1b-AR and α1bd-AR. Recording the spontaneous alternation behavior in a Y-maze assessed spatial working memory performance. Increased spontaneous alternation were seen for α1b-AR-KO mice compared to WT mice and α1bd-AR-KO mice in the Y-maze. The number of arm entries was unchanged among groups. The results indicate that α1b-AR is involved in modulation of spatial working memory fun … More ctions. The neurohypophysial hormone vasopressin(VP) is a key regulator of the hypothalamic-pituitary-adrenal axis and has major effects on anxiety behavior. The vasopressin V1b receptor(V1bR) is specifically expressed in pituitary corticotrophs and mediates the stimulatory effect of VP on adrenocorticotropin release. In order to investigate the role of the V1bR for anxiety behavior, knockout mice deficient of the V1bR was used. In the elevated plus-maze test, V1bR-KO mice increased the time spent in the open arm and the percentage of open arm entries compared to WT mice. There was no difference between the two groups in the number of the total arm entries. In the next study, we have investigated the effects of SSRI and SNRI on V1bR-KO mice using the elevated plus-maze test of anxiety. Neither SSRI nor SNRI changed the time spent in the open arm, the percentage of open arm entries, and the number of the total arm entries. These results suggest that V1bR may contribute to the serotonergic system for the anxiety behavior. Less

通过分子克隆鉴定出3种α1肾上腺素受体（AR）亚型。一些研究分析了α1-ARs在中枢神经系统中的功能作用。最近的研究表明α1- ar参与运动、认知功能和运动活动的控制。然而，人们对α1-AR不同亚型在大脑中的功能作用知之甚少。为了明确α1-AR亚型在学习行为中的作用，我们使用α1b-AR和α1bd-AR缺失的敲除小鼠。研究对象是α1b-AR和α1bd-AR缺失的雄性野生型（WT）小鼠和敲除型（KO）小鼠。通过记录y型迷宫中自发的交替行为来评估空间工作记忆的表现。与WT小鼠和α1bd-AR-KO小鼠相比，y迷宫中α1b-AR-KO小鼠的自发交替增加。各组间手臂的数量没有变化。结果表明，α1b-AR参与了空间工作记忆的调节。垂体神经激素抗利尿激素（VP）是下丘脑-垂体-肾上腺轴的关键调节因子，对焦虑行为有重要影响。抗利尿激素V1b受体（V1bR）在垂体促肾上腺皮质激素中特异性表达，介导VP对促肾上腺皮质激素释放的刺激作用。为了研究V1bR在焦虑行为中的作用，我们使用了V1bR缺失的敲除小鼠。在升高的+迷宫测试中，与WT小鼠相比，V1bR-KO小鼠在张开臂中花费的时间和张开臂进入的百分比增加。两组之间的总臂入数没有差异。在接下来的研究中，我们使用焦虑升高+迷宫测试来研究SSRI和SNRI对V1bR-KO小鼠的影响。SSRI和SNRI都没有改变在开放组中花费的时间、开放组进入的百分比和总进入的数量。这些结果表明V1bR可能参与了焦虑行为的血清素能系统。少