Investigation for function of vasopressin V1b and α1-adrenoceptors in the central nervous system under stress condition

通过分子克隆鉴定了α1肾上腺素能受体(AR)的3种亚型。一些研究分析了α-1-ARs在中枢神经系统中的功能作用。最近的数据表明，α1-AR参与了运动、认知功能和运动活动的控制。然而，人们对不同的α1-AR亚型在大脑中的功能作用知之甚少。为了阐明α1-AR亚型在学习行为中的作用，采用了α1b-AR和α1BD-AR缺陷基因敲除小鼠。研究对象为雄性野生型(WT)小鼠和α1b-AR和α1BD-AR缺陷基因敲除(KO)小鼠。在Y迷宫中记录自发交替行为以评估空间工作记忆表现。在Y迷宫中，α1b-AR-KO小鼠比WT小鼠和α1BD-AR-KO小鼠的自发交替次数增加。ARM条目的数量在不同的组中没有变化。结果表明，α1b-AR参与了空间工作记忆Fun…的调制更多的蛋糕。神经垂体激素加压素(VP)是下丘脑-垂体-肾上腺轴的关键调节因子，对焦虑行为有重要影响。血管加压素V1b受体(V1bR)特异性表达于垂体促肾上腺皮质激素细胞，介导VP对促肾上腺皮质激素释放的刺激作用。为了研究V1bR在焦虑行为中的作用，采用了V1bR基因敲除小鼠。在高架正迷宫测试中，与WT小鼠相比，V1bR-KO小鼠在开放手臂上花费的时间和开放手臂进入的百分比都增加了。在ARM条目总数上，两组之间没有差异。在下一项研究中，我们使用焦虑的高架正迷宫测试来研究SSRI和SNRI对V1bR-KO小鼠的影响。SSRI和SNRI都没有改变在开放手臂中花费的时间、开放手臂条目的百分比和手臂条目总数。这些结果提示，V1bR可能参与了焦虑行为的5-羟色胺能系统。较少

Three α1-adrenoceptor(AR) subtypes have been identified by molecular cloning. Several studies have analyzed the functional roles of α1-ARs in the central nervous system. Recent data suggest that the α1-ARs are involved in locomotion, cognitive functions and in the control of motor activity. However, very little is known about the functional role of distinct α1-AR subtypes in the brain. In order to clarify the role of the α1-AR subtypes for learning behavior, knockout mice deficient of the α1b-AR and α1bd-AR were used. Studies were performed with male wild-type(WT) mice and knockout(KO) mice deficient for the α1b-AR and α1bd-AR. Recording the spontaneous alternation behavior in a Y-maze assessed spatial working memory performance. Increased spontaneous alternation were seen for α1b-AR-KO mice compared to WT mice and α1bd-AR-KO mice in the Y-maze. The number of arm entries was unchanged among groups. The results indicate that α1b-AR is involved in modulation of spatial working memory fun … More ctions. The neurohypophysial hormone vasopressin(VP) is a key regulator of the hypothalamic-pituitary-adrenal axis and has major effects on anxiety behavior. The vasopressin V1b receptor(V1bR) is specifically expressed in pituitary corticotrophs and mediates the stimulatory effect of VP on adrenocorticotropin release. In order to investigate the role of the V1bR for anxiety behavior, knockout mice deficient of the V1bR was used. In the elevated plus-maze test, V1bR-KO mice increased the time spent in the open arm and the percentage of open arm entries compared to WT mice. There was no difference between the two groups in the number of the total arm entries. In the next study, we have investigated the effects of SSRI and SNRI on V1bR-KO mice using the elevated plus-maze test of anxiety. Neither SSRI nor SNRI changed the time spent in the open arm, the percentage of open arm entries, and the number of the total arm entries. These results suggest that V1bR may contribute to the serotonergic system for the anxiety behavior. Less