The study of therapy for radiation-induced thrombocytopenia by using ex vivo expanded-megakaryocyte prepared from auto hematopoietic stem cell.

自体造血干细胞体外扩增巨核细胞治疗放射引起的血小板减少症的研究。

基本信息

  • 批准号:
    15591253
  • 负责人:
  • 金额:
    $ 2.18万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2005
  • 项目状态:
    已结题

项目摘要

1.CD34 antigen is a novel marker for human hematopoietic stem/progenitor cells. Two cell fractions, CD34^<low> and CD34^<high>, were prepared from steady-state human peripheral blood (PB) on the basis of CD34 antigen expression. The content of CD34^+CD45^+ cells in the CD34^<low> and CD34^<high> cell fractions was 74.8% and 88.8%, respectively, and the frequency of thrombopoietin (TPO)-supported colony-forming unit megakaryocytes (CFU-Meg) in the CD34^<low> cell fraction was 1.9 times higher than that in CD34^<high>. The CFU-Meg in CD34^<high> were more radiosensitive than those in CD34^<low>, indicating that steady-state human PB contains different types of CFU-Meg. TPO plus interleukin-3 was the optimal combination for survival of both types of CFU-Meg after X irradiation. These results suggest that multiple combinations with TPO will be helpful in cytokine therapy for post-irradiation thrombocytopenia.2.Mature megakaryocytes induced from the culture of PB CD34^+ cells with TPO are s … More hown high radio sensitivity, however, normal pro-platelet formation occurred after X irradiation. These results suggest that the influence of X irradiation to the end of thrombopoiesis is small.3.Low concentration of Epigallocatechin-3-gallate (EGCg,100 nM), a major green tea polyphenol, reduce the risk factor from radiation damage in megakaryocytopoiesis, suggesting the medical application of EGCg for the treatment of thrombocytopenia.4.CFU-Meg are more radiosensitive progenitors to cabon ion beam than X-ray. The induction of γ-H2AX, a marker of DNA double strand break (DSB), by carbon ion beam in CD34^+ cells was not enhanced by cytokine treatment such as that of X-ray. These results showed that the DSB repair of CFU-Meg by cytokine was lesser in progenitors to carbon ion beam than X-ray.5.A combination of IL-3 plus TPO would be an optimal choice for the regeneration of megakaryocytopoiesis and thrombopoiesis from X-irradiated stem cells. Our results suggest that nearly normal megakaryocytopoiesis and thrombopoiesis can be induced from X-irradiated stem cells by stimulation with appropriate cytokine combinations. Less
1.CD34抗原是一种新的造血干/祖细胞标志物。基于<low><high>CD 34抗原表达,从稳态人外周血(PB)中制备两种细胞组分,CD 34+和CD 34+。CD 34 ^+ CD 45 ^+细胞在CD 34 ^<low>和CD 34 ^<high>细胞组分中的含量分别为74.8%和88.8%,CD 34 ^细胞组分中血小板生成素(TPO)支持的集落形成单位巨核细胞(CFU-Meg)的频率<low>是CD 34 ^中的1.9倍<high>。CD_(34)~+中CFU-Meg的<high>放射敏感性高于CD_(34)~+中CFU-Meg的放射敏感<low>性,表明稳定状态的人PB中含有不同类型的CFU-Meg。TPO + IL-3是X射线照射后两种CFU-Meg存活的最佳组合。以上结果提示,TPO的多次联合应用将有助于细胞因子治疗照射后血小板减少症。 ...更多信息 显示高放射敏感性,然而,正常的前血小板形成后,X射线照射。3.低浓度的表没食子儿茶素没食子酸酯(EGCg,100 nM)(一种主要的绿色茶多酚)可降低巨核细胞生成中辐射损伤的危险因素,提示EGCg在治疗血小板减少症方面的医学应用。细胞因子处理(如X射线)并不能增强碳离子束诱导的CD 34 ^+细胞γ-H2 AX(DNA双链断裂(DSB)标志物)的表达。这些结果表明,细胞因子对CFU-Meg的DSB修复作用较X射线弱。5. IL-3联合TPO是X射线照射后造血干细胞再生巨核细胞和血小板的最佳选择。我们的研究结果表明,几乎正常的巨核细胞生成和血小板生成可以诱导从X射线照射的干细胞刺激与适当的细胞因子组合。少

项目成果

期刊论文数量(105)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Radiation sensitivity of hematopoietic stem/progenitor cells and cytokine action.
造血干/祖细胞的辐射敏感性和细胞因子作用。
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    I.Kashiwakura et al.;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;T.Hamasu et al.;I.Kashiwakura et al.;D.Iizuka et al.;柏倉 幾郎;柏倉 幾郎;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;D.Iizuka et al.;I.Kashiwakura et al.
  • 通讯作者:
    I.Kashiwakura et al.
A novel anticancer ribonucleoside, 1-(3-C-ethyl-β-D-ribo-pentofuranosyl) cytosine, enhances radidaion-induced cell death in tumor cells.
一种新型抗癌核糖核苷 1-(3-C-乙基-β-D-核糖-呋喃戊糖基) 胞嘧啶可增强肿瘤细胞中辐射诱导的细胞死亡。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    I.Kashiwakura et al.;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;T.Hamasu et al.;I.Kashiwakura et al.;D.Iizuka et al.;柏倉 幾郎;柏倉 幾郎;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;D.Iizuka et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;T.Hamasu et al.;I.Kashiwakura et al.;D.Iizuka et al.;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;O.Inanami et al.
  • 通讯作者:
    O.Inanami et al.
Radioprotective synergistic action of cytokines on the clonalgrowth of x-irradiated human megakaryocytic progenitor cells
细胞因子对X射线照射的人巨核细胞祖细胞克隆生长的辐射防护协同作用
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    0
  • 作者:
    I.Kashiwakura et al.;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;T.Hamasu et al.;I.Kashiwakura et al.;D.Iizuka et al.;柏倉 幾郎;柏倉 幾郎;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;D.Iizuka et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;T.Hamasu et al.;I.Kashiwakura et al.;D.Iizuka et al.;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;O.Inanami et al.;S.J.Hossinimehr et al.;柏倉 幾郎;柏倉 幾郎;I.Kashiwakura et al.;O.Inanami et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;柏倉 幾郎;柏倉 幾郎 他;I.Kashiwakura et al.;I.Kashiwakura et al.;柏倉 幾郎;I.Kashiwakura et al.;I.Kashiwakura et al.
  • 通讯作者:
    I.Kashiwakura et al.
Post-irradiation hypoxic incubation of X-irradiated MOLT-4 cells reduces apoptotic cell death by changing the intracellular redox state and modulating SAPK/JNK pathways
  • DOI:
    10.1007/s10495-005-1888-x
  • 发表时间:
    2005-05-01
  • 期刊:
  • 影响因子:
    7.2
  • 作者:
    Hamasu, T;Inanami, O;Kuwabara, M
  • 通讯作者:
    Kuwabara, M
造血幹・前駆細胞の放射線感受性とサイトカインの作用
造血干/祖细胞的放射敏感性及细胞因子的影响
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    I.Kashiwakura et al.;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;S.Monzen et al.;I.Kashiwakura et al.;I.Kashiwakura et al.;T.Hamasu et al.;I.Kashiwakura et al.;D.Iizuka et al.;柏倉 幾郎
  • 通讯作者:
    柏倉 幾郎
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KASHIWAKURA Ikuo其他文献

KASHIWAKURA Ikuo的其他文献

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{{ truncateString('KASHIWAKURA Ikuo', 18)}}的其他基金

Protective effects of a combination of multiple pharmaceutical drugs on the survival of mice exposed to lethal ionizing radiation
多种药物组合对暴露于致命电离辐射的小鼠存活的保护作用
  • 批准号:
    24659553
  • 财政年份:
    2012
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
The development of multifunctional drug for magnetic resonance imaging diagnosis that uses metallic complex liquid crystal compound
金属络合物液晶化合物用于磁共振成像诊断的多功能药物的开发
  • 批准号:
    22659216
  • 财政年份:
    2010
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
The identification of factor (s) which regulate individual radiosensitivity of human hematopoietic stem/progenitor cells and its application
人造血干/祖细胞个体放射敏感性调节因子的鉴定及其应用
  • 批准号:
    21390336
  • 财政年份:
    2009
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Placental/Umbilical cord blood-derived mesenchymal stem cell-likestroma cells support the hematopoietic recovery of X-irradiated human CD34+ cells
胎盘/脐带血来源的间充质干细胞样基质细胞支持 X 射线照射的人 CD34 细胞的造血恢复
  • 批准号:
    18390327
  • 财政年份:
    2006
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Action of optimum cytokine for the recovery of X-irradiated human hematopoietic stem and progenitor cells
最佳细胞因子对 X 射线照射的人类造血干细胞和祖细胞恢复的作用
  • 批准号:
    12672115
  • 财政年份:
    2000
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

The Phenomenon of Stem Cell Aging according to Methylation Estimates of Age After Hematopoietic Stem Cell Transplantation
根据造血干细胞移植后甲基化年龄估算干细胞衰老现象
  • 批准号:
    23K07844
  • 财政年份:
    2023
  • 资助金额:
    $ 2.18万
  • 项目类别:
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The pathophysiology and therapeutic approaches of graft-versus-host disease following allogeneic hematopoietic stem cell transplantation with a focus on the role of oral microbiota.
异基因造血干细胞移植后移植物抗宿主病的病理生理学和治疗方法,重点关注口腔微生物群的作用。
  • 批准号:
    23K15301
  • 财政年份:
    2023
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Psychological and social support for patients and their family members after hematopoietic stem cell transplantation who visit multiple outpatient clinics
为造血干细胞移植后到多个门诊就诊的患者及其家属提供心理和社会支持
  • 批准号:
    23K16481
  • 财政年份:
    2023
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Investigating hematopoietic stem cell dysfunction during sickle cell disease
研究镰状细胞病期间的造血干细胞功能障碍
  • 批准号:
    10681829
  • 财政年份:
    2023
  • 资助金额:
    $ 2.18万
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Regulation and function of aged hematopoietic stem cell (HSC) niche
衰老造血干细胞(HSC)生态位的调节和功能
  • 批准号:
    10723396
  • 财政年份:
    2023
  • 资助金额:
    $ 2.18万
  • 项目类别:
Elucidating the role of DCAF7 on hematopoietic stem cell maintenance
阐明 DCAF7 对造血干细胞维持的作用
  • 批准号:
    10785443
  • 财政年份:
    2023
  • 资助金额:
    $ 2.18万
  • 项目类别:
The physiological and evolutionary significance of the limited site and timing of hematopoietic stem cell development
造血干细胞发育的有限位点和时间的生理和进化意义
  • 批准号:
    23H02941
  • 财政年份:
    2023
  • 资助金额:
    $ 2.18万
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    Grant-in-Aid for Scientific Research (B)
Reprogramming Gene Regulatory Networks to a Hematopoietic Stem Cell State
将基因调控网络重编程为造血干细胞状态
  • 批准号:
    10716641
  • 财政年份:
    2023
  • 资助金额:
    $ 2.18万
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The Role of Hsf1 in Hematopoietic Stem Cell Aging
Hsf1在造血干细胞衰老中的作用
  • 批准号:
    10749675
  • 财政年份:
    2023
  • 资助金额:
    $ 2.18万
  • 项目类别:
Decoupling Hydrogel Stiffness and Diffusivity for Hematopoietic Stem Cell Culture and Differentiation
用于造血干细胞培养和分化的水凝胶刚度和扩散率的解耦
  • 批准号:
    10647478
  • 财政年份:
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