The study of therapy for radiation-induced thrombocytopenia by using ex vivo expanded-megakaryocyte prepared from auto hematopoietic stem cell.
The study of therapy for radiation-induced thrombocytopenia by using ex vivo expanded-megakaryocyte prepared from auto hematopoietic stem cell.
自体造血干细胞体外扩增巨核细胞治疗放射引起的血小板减少症的研究。
批准号：
15591253
负责人：
KASHIWAKURA Ikuo
金额：
$2.18万
依托单位：
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
项目状态：
已结题
起止时间：
2003 至 2005
中文摘要
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英文摘要
1.CD34 antigen is a novel marker for human hematopoietic stem/progenitor cells. Two cell fractions, CD34^<low> and CD34^<high>, were prepared from steady-state human peripheral blood (PB) on the basis of CD34 antigen expression. The content of CD34^+CD45^+ cells in the CD34^<low> and CD34^<high> cell fractions was 74.8% and 88.8%, respectively, and the frequency of thrombopoietin (TPO)-supported colony-forming unit megakaryocytes (CFU-Meg) in the CD34^<low> cell fraction was 1.9 times higher than that in CD34^<high>. The CFU-Meg in CD34^<high> were more radiosensitive than those in CD34^<low>, indicating that steady-state human PB contains different types of CFU-Meg. TPO plus interleukin-3 was the optimal combination for survival of both types of CFU-Meg after X irradiation. These results suggest that multiple combinations with TPO will be helpful in cytokine therapy for post-irradiation thrombocytopenia.2.Mature megakaryocytes induced from the culture of PB CD34^+ cells with TPO are s … More hown high radio sensitivity, however, normal pro-platelet formation occurred after X irradiation. These results suggest that the influence of X irradiation to the end of thrombopoiesis is small.3.Low concentration of Epigallocatechin-3-gallate (EGCg,100 nM), a major green tea polyphenol, reduce the risk factor from radiation damage in megakaryocytopoiesis, suggesting the medical application of EGCg for the treatment of thrombocytopenia.4.CFU-Meg are more radiosensitive progenitors to cabon ion beam than X-ray. The induction of γ-H2AX, a marker of DNA double strand break (DSB), by carbon ion beam in CD34^+ cells was not enhanced by cytokine treatment such as that of X-ray. These results showed that the DSB repair of CFU-Meg by cytokine was lesser in progenitors to carbon ion beam than X-ray.5.A combination of IL-3 plus TPO would be an optimal choice for the regeneration of megakaryocytopoiesis and thrombopoiesis from X-irradiated stem cells. Our results suggest that nearly normal megakaryocytopoiesis and thrombopoiesis can be induced from X-irradiated stem cells by stimulation with appropriate cytokine combinations. Less
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Radiation sensitivity of hematopoietic stem/progenitor cells and cytokine action.
造血干/祖细胞的辐射敏感性和细胞因子作用。
造血干/祖细胞的辐射敏感性和细胞因子作用。
DOI：
--
发表时间：
2005
期刊：
Jpn.J.Cancer Clin. 51(5)
影响因子：
--
作者：
[I.Kashiwakura et al., S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., T.Hamasu et al., I.Kashiwakura et al., D.Iizuka et al., 柏倉 幾郎, 柏倉 幾郎, S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., D.Iizuka et al., I.Kashiwakura et al.]
通讯作者：
I.Kashiwakura et al.
A novel anticancer ribonucleoside, 1-(3-C-ethyl-β-D-ribo-pentofuranosyl) cytosine, enhances radidaion-induced cell death in tumor cells.
一种新型抗癌核糖核苷 1-(3-C-乙基-β-D-核糖-呋喃戊糖基) 胞嘧啶可增强肿瘤细胞中辐射诱导的细胞死亡。
一种新型抗癌核糖核苷 1-(3-C-乙基-β-D-核糖-呋喃戊糖基) 胞嘧啶可增强肿瘤细胞中辐射诱导的细胞死亡。
DOI：
--
发表时间：
2004
期刊：
Radiat. Res. 162(6)
影响因子：
--
作者：
[I.Kashiwakura et al., S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., T.Hamasu et al., I.Kashiwakura et al., D.Iizuka et al., 柏倉 幾郎, 柏倉 幾郎, S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., D.Iizuka et al., I.Kashiwakura et al., I.Kashiwakura et al., I.Kashiwakura et al., T.Hamasu et al., I.Kashiwakura et al., D.Iizuka et al., S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., I.Kashiwakura et al., O.Inanami et al.]
通讯作者：
O.Inanami et al.
Radioprotective synergistic action of cytokines on the clonalgrowth of x-irradiated human megakaryocytic progenitor cells
细胞因子对X射线照射的人巨核细胞祖细胞克隆生长的辐射防护协同作用
细胞因子对X射线照射的人巨核细胞祖细胞克隆生长的辐射防护协同作用
DOI：
--
发表时间：
2003
期刊：
12^<th> International Congress of Radiation Research Final program & book of abstracts
影响因子：
--
作者：
[I.Kashiwakura et al., S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., T.Hamasu et al., I.Kashiwakura et al., D.Iizuka et al., 柏倉 幾郎, 柏倉 幾郎, S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., D.Iizuka et al., I.Kashiwakura et al., I.Kashiwakura et al., I.Kashiwakura et al., T.Hamasu et al., I.Kashiwakura et al., D.Iizuka et al., S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., I.Kashiwakura et al., O.Inanami et al., S.J.Hossinimehr et al., 柏倉 幾郎, 柏倉 幾郎, I.Kashiwakura et al., O.Inanami et al., I.Kashiwakura et al., I.Kashiwakura et al., 柏倉 幾郎, 柏倉 幾郎 他, I.Kashiwakura et al., I.Kashiwakura et al., 柏倉 幾郎, I.Kashiwakura et al., I.Kashiwakura et al.]
通讯作者：
I.Kashiwakura et al.
DOI：
10.1007/s10495-005-1888-x
发表时间：
2005-05-01
期刊：
APOPTOSIS
影响因子：
7.2
作者：
[Hamasu, T, Inanami, O, Kuwabara, M]
通讯作者：
Kuwabara, M
造血幹・前駆細胞の放射線感受性とサイトカインの作用
造血干/祖细胞的放射敏感性及细胞因子的影响
造血干/祖细胞的放射敏感性及细胞因子的影响
DOI：
--
发表时间：
2005
期刊：
癌の臨床 51(5)
影响因子：
--
作者：
[I.Kashiwakura et al., S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., S.Monzen et al., I.Kashiwakura et al., I.Kashiwakura et al., T.Hamasu et al., I.Kashiwakura et al., D.Iizuka et al., 柏倉 幾郎]
通讯作者：
柏倉 幾郎
共 29 条
Protective effects of a combination of multiple pharmaceutical drugs on the survival of mice exposed to lethal ionizing radiation
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批准号：24659553
-
项目类别：Grant-in-Aid for Challenging Exploratory Research
-
资助金额：$2.33万
-
财政年份：2012
-
负责人：KASHIWAKURA Ikuo
-
依托单位：
The development of multifunctional drug for magnetic resonance imaging diagnosis that uses metallic complex liquid crystal compound
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批准号：22659216
-
项目类别：Grant-in-Aid for Challenging Exploratory Research
-
资助金额：$2.08万
-
财政年份：2010
-
负责人：KASHIWAKURA Ikuo
-
依托单位：
The identification of factor (s) which regulate individual radiosensitivity of human hematopoietic stem/progenitor cells and its application
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批准号：21390336
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项目类别：Grant-in-Aid for Scientific Research (B)
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资助金额：$6.99万
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财政年份：2009
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负责人：KASHIWAKURA Ikuo
-
依托单位：
Placental/Umbilical cord blood-derived mesenchymal stem cell-likestroma cells support the hematopoietic recovery of X-irradiated human CD34+ cells
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批准号：18390327
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项目类别：Grant-in-Aid for Scientific Research (B)
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资助金额：$5.67万
-
财政年份：2006
-
负责人：KASHIWAKURA Ikuo
-
依托单位：
Action of optimum cytokine for the recovery of X-irradiated human hematopoietic stem and progenitor cells
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批准号：12672115
-
项目类别：Grant-in-Aid for Scientific Research (C)
-
资助金额：$1.98万
-
财政年份：2000
-
负责人：KASHIWAKURA Ikuo
-
依托单位：
海外基金