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Evaluation of peritumoral fiber structures of N-ethyl-N-nitrosourea-induced rat brain glioma using Diffusion tensor MR imaging : comparison between vector map and histology

使用扩散张量磁共振成像评估 N-乙基-N-亚硝基脲诱导的大鼠脑胶质瘤的瘤周纤维结构：矢量图与组织学之间的比较

基本信息

批准号：
15591267
负责人：
ITO Ryuta
金额：
$ 2.05万
依托单位：
SHIGA University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

We investigated fiber structures in the peripheral zone of N-ethyl-N-nitrosourea (ENU)-induced rat brain glioma by comparison between diffusion tensor MR (DT-MR) images and neurofilament (NF) immunostained histological sections.In this study, six Fisher-334 rats, which had been treated transplacentally with ENU, underwent in vitro MR imaging. All procedures met institutional Laboratory Animal Care and Use Committee approval. The experiments were performed on a horizontal 7T/40 cm magnet equipped with a 12cm gradient set up to 400mT/m interfaced to an experimental NMR system. The fixed brain was put into the tube filled with 0.1M phosphate buffer for in vitro DT-MR imaging. Acquisition parameters were 2000/36/12-14 (TR msec/TE msec/averaging), 24mm field of view, 0.5mm slice thickness, 256x256 scan matrix (0.0938x0.0938x0.5mm^3). A data set of diffusion-weighted images with b-value=1027sec/mm^2 in six different noncolinear directions and T2-weighted images was obtained. From the diffusi … More on-tensor data, DT-MR imaging-based color maps and vector maps were generated. After DT-MR imaging, neurofilament immunohistochemistry was performed using the avidin-biotin-peroxidase complex method. We visually evaluated peritomoral white matter architecture described by the vector maps in comparison with corresponding neurofilament (NF) immunostained histological sections.We obtained the following results. (1)In normal white matter, the vector map could depict well-ordered vector bars corresponding ordered NF structures in immunostained histological sections. (2)The vector map could clearly show peritumoral widening and disruption of the vector bars along the border between normal white matter and tumor, which were confirmed by the histological sections. (3)The vector maps showed well-ordered vector bars in the peritumoral area with abnormal hyper intensity on T2-weighted images, where histology revealed normal well-ordered NF bundles without tumor cells in the area. (4)The vector map could not fully describe scattered residual NF bundle pieces in the tumor. Less

本研究通过比较N-乙基-N-亚硝基脲（ENU）诱导的大鼠脑胶质瘤的扩散张量MR（DT-MR）图像和神经丝（NF）免疫组织化学染色切片，观察了ENU诱导的6只Fisher-334大鼠脑胶质瘤外周带的纤维结构。所有程序均符合机构实验动物管理和使用委员会的批准。实验在水平7 T/40 cm磁体上进行，该磁体配备有12 cm梯度，设置为高达400 mT/m，与实验NMR系统连接。将固定后的脑组织放入装有0.1M磷酸盐缓冲液的试管中进行体外DT-MR成像。采集参数为2000/36/12-14（TR msec/TE msec/平均值），24 mm视野，0.5 mm层厚，256 x 256扫描矩阵（0.0938 x 0.0938 x 0.5 mm^3）。在6个不同的非共线方向上获得b值= 1027 sec/mm^2的扩散加权图像和T2加权图像的数据集。从扩散 ...更多信息非张量数据、基于DT-MR成像的彩色图和矢量图被生成。DT-MR成像后，使用抗生物素蛋白-生物素-过氧化物酶复合物法进行神经丝免疫组化。我们通过与相应的神经丝（NF）免疫染色组织切片比较，直观地评价了由矢量图描述的瘤周白色物质结构。(1)In在正常白色物质中，矢量图可以描绘与免疫染色组织切片中的有序NF结构相对应的有序矢量条。（2）矢量图可清晰显示正常白色物质与肿瘤交界处的瘤周增宽和沿着分布的矢量条断裂，并与组织学切片相吻合。（3）矢量图显示瘤周区域的矢量条排列整齐，T2加权像上呈异常高信号，组织学检查显示该区域的NF束排列整齐，无肿瘤细胞。（4）矢量图不能完全描述肿瘤内散在残留的NF束碎片。少