DIFFUSION TENSOR MR IMAGING OF A MOUSE FASD MODEL

小鼠 FASD 模型的扩散张量 MR 成像

• 批准号: 7726178
• 负责人: KATHLEEN K SULIK
• 金额: $ 1.55万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7726178
• 关键词: A Mouse; Acute; Adverse effects; Affect; Brain; Child; Chronic; Clinical; Computer Retrieval of Information on Scientific Projects Database; Craniofacial Abnormalities; Defect; Development; Diagnosis; Diagnostic tests; Diffusion; Diffusion Magnetic Resonance Imaging; Disease model; Documentation; Dysmorphology; Embryo; Ethanol; Face; Fetal Alcohol Exposure; Fetal Alcohol Spectrum Disorder; Fetal Alcohol Syndrome; Fiber; Funding; Future; Goals; Grant; Human; Incidence; Individual; Institution; Investigation; Magnetic Resonance Imaging; Modeling; Mus; Pattern; Public Health; Research; Research Personnel; Resolution; Resources; Source; Staging; Testing; Time; United States National Institutes of Health; Work; design; fetal; high throughput screening; mouse model

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Fetal Alcohol Spectrum Disorders (FASD), significant components of which are CNS and craniofacial abnormalities, are a major public health problem. While eliminating FASD is the ultimate goal for clinical and basic FASD research, we recognize that in the near future, adverse effects from prenatal ethanol exposure will persist. To better diagnose and treat affected individuals, a more complete understanding of the full spectrum of the ethanol-induced abnormalities is needed. The proposed investigations are designed to contribute significantly toward meeting this need. For this work, Diffusion Tensor Imaging (DTI), which allows CNS fiber tract analyses at a high resolution (60- micron or less isotropic), will be applied to the study of an FASD mouse model. Previous research using this model established critical exposure times that yield facial and CNS abnormalities consistent with full-blown Fetal Alcohol Syndrome, as well as other components of FASD. The proposed studies will employ this model and both acute and chronic ethanol treatment paradigms to test the overall hypothesis that in mice, ethanol induces structural abnormalities of the brain and face that are consistent with and informative for those in human FASD. To this end, utilizing DTI as high throughput screening platforms, we propose to provide comprehensive documentation and discovery of the ethanol-induced CNS dysmorphology that results from prenatal ethanol exposure at embryonic and early fetal stages of development. It is expected that the structural abnormalities of the brain that are induced by ethanol in mice will reflect the pattern of defects observed in children with FASD, will inform human diagnostic tests, and will provide new information to help reduce the incidence of FASD.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 胎儿酒精谱系障碍（FASD）是一个主要的公共卫生问题，其重要组成部分是中枢神经系统和颅面畸形。虽然消除FASD是临床和基础FASD研究的最终目标，但我们认识到，在不久的将来，产前乙醇暴露的不良影响将持续存在。更好地 为了诊断和治疗受影响的个体，需要更全面地了解乙醇诱导的异常的全谱。拟议的调查旨在为满足这一需求做出重大贡献。对于这项工作，扩散张量成像（DTI），它允许在高分辨率（60- 100）下分析CNS纤维束。 微米或更小的各向同性），将应用于FASD小鼠模型的研究。先前使用该模型的研究建立了关键的暴露时间，这些时间产生与全面的胎儿酒精综合征以及FASD的其他组成部分一致的面部和CNS异常。拟议的研究将采用这种模型， 慢性乙醇治疗范例，以测试总体假设，即在小鼠中，乙醇诱导大脑和面部的结构异常，这与人类FASD中的结构异常一致并提供信息。为此，利用DTI作为高通量筛选平台，我们建议提供全面的文件和发现乙醇诱导的中枢神经系统畸形，结果从产前乙醇暴露在胚胎和早期胎儿发育阶段。预计乙醇诱导的小鼠大脑结构异常将反映在FASD儿童中观察到的缺陷模式，将为人类诊断测试提供信息，并将提供新的信息，以帮助降低FASD的发病率。