虚血再潅流障害における腸管粘膜再生に対する骨髄由来細胞の役割:実験的検討

本实验室以往的研究表明，胎儿小肠移植物在受体组织中不需血管重建即可存活和发育。然而，成人小肠移植物是否能存活以及这些移植物再生和发育的机制仍不清楚，我们研究了成人小肠移植物（ASBG）在移植物浆膜切除术（SS）后是否能在没有血管重建的情况下存活。我们的结果表明，成年小肠移植可以成功地在大鼠没有血管重建，通过去除移植浆膜。此外，SS允许血管内皮生长因子（VEGF）mRNA和随后的ASBG中的VEGF蛋白在移植后很快被诱导，我们还研究了在同一大鼠模型中，粒细胞集落刺激因子（G-CSF）或碱性成纤维细胞生长因子（bFGF）是否能提高移植物的存活率，结果表明，G-CSF和bFGF对移植物的存活有促进作用。CSF和bFGF增强ASBG的血管生成和粘膜表面扩张。

Previous studies in our laboratories have shown that fetal small intestinal graft can survive and develop after transplantation in recipient tissue without any vascular reconstruction. However, whether adult small intestinal graft can survive or not and the mechanisms by which these intestinal grafts regenerate and develop are still unclear.We examined whether adult small bowel grafts (ASBGs) can survive transplantation without vascular reconstruction if graft serosectomy (SS) is performed. Our results suggest that adult small bowel can be transplanted successfully in rats without vascular reconstruction by removing the graft serosa. Furthermore, SS allows vascular endothelial growth factor (VEGF) mRNA and, subsequently, VEGF protein in ASBG to be induced very soon after transplantation, which may contribute to the survival of ASBG transplanted without vascular reconstruction.We also studied that if granulocyte colonystimulating factor (G-CSF) or basic fibroblast growth factor (bFGF) could improve graft survival in the same rat model and the results suggest that G-CSF and bFGF enhance angiogenesis and mucosal surface expansion of the ASBG.