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ASSOCIATION OF GLYCOCONJUGATES EXPRESSION AND CLINICAL SIGNIFICANCE IN PATIENTS WITH METASTATIC CANCER OF THE LIVER

肝转移癌患者中糖缀合物表达及其临床意义的关联

基本信息

批准号：
15591384
负责人：
KARAKO Takashi
金额：
$ 2.24万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591384/
关键词：
Colorectal Cancer live metastasis Metastasis Glycocojugates Lectins Sialic acid Immunohistochemistry Prognosis

项目摘要

Sialoglycoconjugates is thought to play an important role in tumor metastasis and progression. This study involved histochemical analyses of human colorectal cancer and metastatic liver cancer tissues to detect tumor-associated sialoglycoconjugates and to clarify the relationship between their histochemical expression and the metastatic potential of the tumor.Human colorectal cancer and metastatic tissues were subjected histochemical staining using sialic acid-binding lectins and anti-KL-6 mucin monoclonal antibody. Relationship between the staining characteristics and clinicopathological parameters were statistically analyzed. Aberrant expression of sialoglycoconjugates was detected in colorectal cancer tissues by means of lectin-histochemical staining. The high level staining was related to aggressive tumor behavior such as lymphatic metastasis. On the other hand, KL-6 mucin was detected in colorectal cancer tissues but not in normal tissues surrounding the tumors. The expression was significantly frequent not only in cases of lymph node metastasis but also in those of liver metastasis. In addition, KL-6 antigen was detected in metastatic liver cancer tissues with high frequency (53/56, 95%).In conclusion, the present results indicate that tumor-associated sialoglycoconjugates appear in human colorectal cancer and metastatic liver tissues and suggest that the aberrant expression of sialoglycoconjugates, especially KL-6 mucin, is closely related to unfavorable behavior such as liver metastasis of colorectal cancer.

唾液酸糖缀合物被认为在肿瘤转移和进展中发挥重要作用。本研究采用唾液酸结合凝集素和抗KL-6粘蛋白单克隆抗体对人结直肠癌和转移性肝癌组织进行组织化学染色，检测肿瘤相关唾液酸糖缀合物的表达，并探讨其表达与肿瘤转移潜能的关系。统计分析其染色特征与临床病理参数的关系。通过凝集素组织化学染色检测结直肠癌组织中唾液酸糖缀合物的异常表达。高水平的染色与肿瘤的侵袭性行为如淋巴结转移有关。另一方面，KL-6粘蛋白在结直肠癌组织中检测到，但在肿瘤周围的正常组织中未检测到。不仅在淋巴结转移的病例中，而且在肝转移的病例中表达显著频繁。另外，KL-6抗原在转移性肝癌组织中的检出率较高（53/56，95%），提示结直肠癌和转移性肝组织中存在肿瘤相关的唾液酸糖缀合物，唾液酸糖缀合物尤其是KL-6粘蛋白的异常表达与结直肠癌肝转移等不良行为密切相关。