ASSOCIATION OF GLYCOCONJUGA.TES EXPRESSION APjO) CUNICAL SIGNIFICANCE IN HUMAN GASTRIC CANCER

糖复合物表达协会 APjO) 在人类胃癌中的重要意义

• 批准号: 13671286
• 负责人: KARAKO Takashi
• 金额: $ 2.24万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671286/
• 关键词: Gastric Cancer; Immunohistochemistry; Glycocojugates; Lectins; Growth; Invasion; Metastasis; Prognosis

Cell surface glycosylation is dramatically changed during oncogenesis, and is considered to correlate with the malignant potential and development of cancer. Therefore, detection of carbohydrates of tumor tissues with lectins and antibodies against tumor-associated carbohydrate antigens may help to evaluate the metastatic potential and aggressive tumor behavior.In this study, we performed histochemical and biochemical analysis of human gastric cancer tissues using lectins to investigate relationship between sialylation of glycoconjugates and clinicopathological characteristics of the tumor. We describe here that Maackia amurensis leukoagglutinin (MAL)-positive glycoconjugates only appear in gastric tumor tissues. On the other hand, no staining was detected in the non-cancerous region. And the high level expression of MAL-positive staining was significantly frequent in cases of lymph node metastasis, lymphatic vessel invasion, and venous invasion, as well as in cases of advanced tumors. Furthermore, prognosis analysis showed that the high level expression of MAL-positive glycoconjugates is an independent factor in a shorter survival together with the depth of invasion and the venous invasion.In conclusion, the present results indicate that MAL-positive glycoconjugates appear in gastric tumor tissu es and suggest that the abnormal glycosylation detected using MAL closely related to unfavorable behavior of gastric cancer. Therefore, MAL would be an effective tool used to determine the prognosis of patients with gastric cancer.

细胞表面糖基化在肿瘤发生过程中发生显著变化，并且被认为与癌症的恶性潜能和发展相关。因此，检测肿瘤组织中的糖与凝集素和肿瘤相关的糖抗原的抗体可能有助于评估转移潜力和侵袭性肿瘤behavior.In本研究中，我们进行了组织化学和生物化学分析人胃癌组织中使用凝集素糖结合物的唾液酸化和肿瘤的临床病理特征之间的关系。我们在这里描述怀槐白细胞凝集素（MAL）阳性糖复合物只出现在胃肿瘤组织。另一方面，在非癌区域中未检测到染色。在淋巴结转移、淋巴管浸润、静脉浸润及进展期肿瘤中，MAL阳性表达率明显增高。预后分析显示，MAL阳性糖复合物的高表达与胃癌浸润深度、静脉浸润程度有关，是胃癌患者生存期缩短的独立因素，提示MAL阳性糖复合物存在于胃癌组织中，MAL检测的糖基化异常与胃癌的不良行为密切相关。因此，MAL可作为判断胃癌患者预后的有效工具。

项目成果

TANG W, et al.: "Requirement of ceramide for adhesion of Helicobacter pylori to glycosphingolipids"FEBS Lett.. 504. 31-35 (2001)

TANG W 等：“幽门螺杆菌与鞘糖脂粘附所需的神经酰胺”FEBS Lett.. 504. 31-35 (2001)

TANG W, Nakata M, Mafune K, Konishi T, 他5名, Makuuchi M: "Histochemical appearance and prognostic significance of Maackia amurensis leukoagglutinin-positive glycoconjugates in human gastric cancer"European Surgical Research. (in press).

TANG W、Nakata M、Mafune K、Konishi T 和其他 5 人，Makuuchi M：“人胃癌中山毛榉白细胞凝集素阳性结合物的组织化学表现和预后意义”欧洲外科研究（正在出版）。

TANG W, et al.: "Histochemical appearance and prognostic significance of Maackia amurensis leikoagglutinin-positive glycoconjugates in human gastric cancer(Proceedings of the 37th Congress of the European Society for Surgical Research)"Mihaly Boros Ed., M

TANG W, et al.：“人胃癌中猕猴桃凝集素阳性糖缀合物的组织化学表现和预后意义（欧洲外科研究学会第 37 届大会论文集）”Mihaly Boros Ed., M

TANG W, et al.: "Association of histochemical expression of Maackia amurensis leukoagglutinin-positive glycoconjugates with behaviour of human gastric cancer"Histopathology. 42. 239-245 (2003)

TANG W 等人：“山槐白细胞凝集素阳性糖缀合物的组织化学表达与人胃癌行为的关联”组织病理学。

Tang W, et al.: "Histochemical appearance and prognostic significance of Maackia amurensis leukoagglutinin-positive Glycoconjugates in human gastric cancer"Eur Surg Res. 34 (suppl 1). 72-73 (2002)

Tang W 等人：“人胃癌中猕猴桃白凝集素阳性糖缀合物的组织化学表现和预后意义”Eur Surg Res。