CLINICAL SIGNIFICANCE OF CELLULAR EXPRESSION OF KL-6 MUCIN IN CHOLANGIOCRCINOMA

胆管癌中 KL-6 粘蛋白细胞表达的临床意义

• 批准号: 17591379
• 负责人: KARAKO Takashi
• 金额: $ 2.3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591379/
• 关键词: Cholangiocarcinoma; Clinicopathology; Glycocojugates; Mucin; Liver cancer; Immunohistochemistry; Tumor marker; Diagnosis; 糖鎖

Patients with cholangiocarcinoma (CC) has particularly worse prognosis than those with hepatocellular carcinoma (HCC), which is due to the difficulty of the diagnosis of CC in many cases. Thus, it is important to develop an effective tumor marker that can diagnosis CC in early phase and estimate patients' prognosis. In this study, we aimed to elucidate significance of KL-6 mucin in CC patients by immunohistochemical and seralogical analyses. The histochemical analysis showed that positive staining of KL-6 mucin was detected only in CC tissues but not in HCC tissues and normal liver regions of all liver cancer tissues. Serological analysis showed serum KL-6 mucin level was elevated in CC patients compared with healthy individuals, HCC patients and metastatic liver cancer patients (P<0.0001) and that CC patients were able to be discrminate from other liver cancer patients by setting a cut-off value at 248 U/mL. These results indicated that KL-6 mucin might be useful in the histochemical detection and serological discrimination of CC patients. Furthermore, the immunohistochemical analysis of metastatic liver cancer tissues displayed that all tissues were positive for KL-6 mucin, but the characteristics of KL-6 expression is not the same as CC tissues as well as serum KL-6 mucin level. Moreover, experiment using various kinds of colon cancer lines showed that the localization of KL-6 mucin was significantly related to the cancer behavior such as cancer cell morphology and metastatic potential. The present study indicates that KL-6 is prospective tool for CC diagnosis and elucidation of various gastrointestinal cancer behaviors.

胆管癌（CC）患者的预后比肝细胞癌（HCC）患者更差，这是由于在许多情况下CC的诊断困难。因此，寻找一种有效的肿瘤标志物，对早期诊断CC和判断预后具有重要意义。本研究旨在通过免疫组化和血清学分析，阐明KL-6粘蛋白在CC患者中的意义。组织化学分析显示，KL-6粘蛋白仅在CC组织中呈阳性表达，而在所有肝癌组织中的HCC组织和正常肝组织中均未见阳性表达。血清学分析显示CC患者血清KL-6粘蛋白水平明显高于健康人、HCC患者和转移性肝癌患者（P<0.0001），以248 U/mL为界值可将CC患者与其他肝癌患者区分开来。提示KL-6粘蛋白可用于CC的组织化学检测和血清学鉴别。转移性肝癌组织中KL-6粘蛋白的免疫组化分析显示，所有组织均呈阳性表达，但KL-6的表达特点与癌组织及血清KL-6粘蛋白水平不同。此外，使用各种结肠癌细胞系的实验表明，KL-6粘蛋白的定位与癌细胞形态和转移潜能等癌症行为显著相关。本研究表明KL-6是一种有前景的诊断CC和阐明各种胃肠道肿瘤行为的工具。

项目成果

Simultaneous resection of liver cell adenomas and an intrahepatic portosystemic venous shunt with elevation of serum PIVKA-II level.

同时切除肝细胞腺瘤和肝内门体静脉分流术，血清 PIVKA-II 水平升高。

• 发表时间: 2006
• 期刊: J Gastroenterol 41(9)
• 作者: 諸角誠人;小川由英;他;大神一浩;Seyama Y
• 通讯作者: Seyama Y

Clinical significance of subcellular lacalization of KL-6 mucin in primary colorectal adenocarcinoma and metastatic tissues.

原发性结直肠腺癌和转移组织中KL-6粘蛋白亚细胞定位的临床意义。

• 发表时间: 2006
• 期刊: World J Gastroenterol 12(1)
• 作者: Guo Q;TANG W;他2名;Kokudo N;他4名;Makuuchi M
• 通讯作者: Makuuchi M

KL-6 mucin in metastatic liver cancer tissues from primary colorectal carcinoma.

• 发表时间: 2009-01
• 期刊: Hepato-gastroenterology
• 作者: Keming Zhang;Wei Tang;Xian-Jun Qu;Q. Guo;Y. Inagaki;Y. Seyama;H. Abe;R. Gai;N. Kokudo;Y. Sugawara;M. Nakata;M. Makuuchi

Detection of new tumors by intraoperative ultrasonography during repeated hepatic resections for hepatocellular carcinoma.

在肝细胞癌的重复肝切除过程中通过术中超声检测新肿瘤。

• 发表时间: 2007
• 期刊: Arch Surg 142
• 作者: Zhang K;Kokudo N;Hasegawa K;Arita J;Tang W;Aoki T;Imamura H;Sano K;Sugawara Y;Makuuchi M.
• 通讯作者: Makuuchi M.

Epidermal growth factor-mediated growth control of confluent mammary epithelial cells cultured on artificial basement membrane

表皮生长因子介导的人工基底膜上培养的汇合乳腺上皮细胞的生长控制

• 发表时间: 2005
• 期刊: Int J Mol Med 16(3)
• 作者: Guo Q;TANG W;他5名;Nakata M;Fujita-Yamaguchi Y;Makuuchi M
• 通讯作者: Makuuchi M