Establishment of diagnostic system by immunohistochemistory using Tissue Microarray for human gastric cancer

组织微阵列免疫组织化学诊断人胃癌体系的建立

• 批准号: 15591388
• 负责人: YAMADA Hiroyuki
• 金额: $ 1.54万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591388/
• 关键词: Tissue Microarray; immunity histology experiment; Donor block; Array block; Recipient block; cancer-related protein

Forty-one gastric cancer specimens (20 were differentiated adenocarinoma and 21 undifferentiated) were analyzed in this study. Using Manual tissue arrayer, 2 grafts (2mm in diameter), which showed representative histological feature, were taken out as from each specimen ("donor block"). Forty-eight grafts were put per "recipient block". The graft was lined up in the donor block by histological type and tumor stage. In cutting the recipient block, successfully cut into 3 micro-meter thickness without the grafts in the recipient block being destroyed. It became clear that an array block well reflected the histological structure of donor blocks in tumor cell and gland density when it was compared with donor blocks. In immunohistochemistory, a positive correlation was observed in p-53, Ki-57 and COX-2 expression intensity between donor and microarray (p<0.0005, <0.0005 and <0.0001, respectively). Therefore, diagnostic system by immunohistochemistory using Tissue Microarray was supposed to be useful for human gastric cancerWe are now evaluating the efficacy of Tissue Microarray by analyzing the expression of other proteins, such as VGEF-C, thymidylate synthase, dihydropyrimidine dehydrogenase, thymidine phosphorylase and so on..

本研究分析了41例胃癌标本（分化型腺癌20例，未分化型21例）。用手动组织点样器从每个标本中取出2个具有代表性组织学特征的移植物（直径2 mm）（“供体块”）。每个“受体块”放置48个移植物。根据组织学类型和肿瘤分期将移植物在供体块中排列。在切割受体块时，成功地切割成3微米厚，而受体块中的移植物未被破坏。与供体组织块相比，阵列组织块在肿瘤细胞密度和腺体密度方面较好地反映了供体组织块的组织学结构。免疫组化结果显示，供体与芯片之间p-53、Ki-57和考克斯-2的表达强度呈正相关（分别为p<0.0005、p <0.0005和p <0.0001）。因此，利用组织芯片进行胃癌的生物化学诊断系统有望成为一种有效的方法。我们目前正在通过分析其他蛋白质的表达，如VGEF-C、胸苷酸合成酶、二氢嘧啶脱氢酶、胸苷磷酸化酶等，来评估组织芯片的诊断效果。

项目成果

E-Selectin-Triggered Recruitment of Endothelial Progenitor Cells to Vascular Endothelium is Significantly Enhanced by Preexisting Plateles.

预先存在的血小板显着增强了 E-选择素触发的内皮祖细胞向血管内皮的募集。

• 发表时间: 2004
• 期刊: Circulation J.Suppl. 68
• 作者: N.Nakamura;Y.Nishiwaki;M.Yoshida;K.Shimokado
• 通讯作者: K.Shimokado

A phase I/II study of tegafur-uracil(UFT) plus oral leucovorin(IV) and biweekly CPT-11 therapy for patients(pts) with metastatic colorectal cancer(CRC).

替加氟尿嘧啶 (UFT) 加口服甲酰四氢叶酸 (IV) 和每两周 CPT-11 治疗转移性结直肠癌 (CRC) 患者的 I/II 期研究。

• 发表时间: 2004
• 期刊: JOURNAL OF CLINICAL ONCOLOGY 22(14): Suppl.S, JUL 15
• 作者: Y.Takano;K.Shimokado;Y.Hata;M.Yoshida;Uetake H
• 通讯作者: Uetake H

Identification of the critical serine residues responsible for phosphorylation of endothelial E-selectin.

鉴定负责内皮 E-选择素磷酸化的关键丝氨酸残基。

• 发表时间: 2004
• 期刊: Circulation J.Suppl. 68
• 作者: M.Nagai;N.Nitta;K.Shimokado;M.Yoshida
• 通讯作者: M.Yoshida

A phase I/II study of tegafur-uracil (UFT) plus oral leucovorin (LV) and biweekly CPT-11 therapy for patients (pts) with metastatic colorectal cancer (CRC).

对转移性结直肠癌 (CRC) 患者 (pts) 进行替加氟尿嘧啶 (UFT) 加口服亚叶酸 (LV) 和每两周一次 CPT-11 治疗的 I/II 期研究。

• 发表时间: 2004
• 期刊: JOURNAL OF CLINICAL ONCOLOGY 22
• 作者: 柿本應貴;小松宏寿;Kakimoto M;Kobayashi H;植竹宏之
• 通讯作者: 植竹宏之

Sphinsosine l-phosphate induced monocyte-endothelial interaction involves PKC and RhoA dependent mechanism

L-磷酸鞘氨醇诱导单核细胞-内皮相互作用涉及 PKC 和 RhoA 依赖机制

• 发表时间: 2004
• 期刊: Circulation J. Suppl. 68
• 作者: A.Kawakami;M.Nagai;A.Tanaka;K.Nakajima;K.Shimokado;M.Yoshida
• 通讯作者: M.Yoshida