Establishment of diagnostic system by immunohistochemistory using Tissue Microarray for human gastric cancer

本文分析了41例胃癌标本(其中20例为分化型腺癌，21例为未分化癌)。使用手动组织排列器，从每个标本中取出2个具有代表性的组织学特征的移植物(“供体块”)，直径2 mm。每个“受体块”放置48个移植物。根据组织学类型和肿瘤分期将移植物排列在供体块内。在切割受体块时，成功地将受体块切割成3微米厚，而受体块中的移植物未被破坏。与供体块相比，阵列块能更好地反映供体块内肿瘤细胞的组织结构和腺体密度。免疫组织化学显示供者和微阵列中P-53、Ki-57和COX-2的表达强度呈正相关(P&lt；0.0005、&lt；0.0005和&lt；0.0001)。因此，基于组织芯片的免疫组织化学诊断系统对人胃癌的诊断具有一定的应用价值。目前，我们正在通过分析血管内皮生长因子-C、胸苷合成酶、二氢嘧啶脱氢酶、胸苷磷酸化酶等蛋白的表达来评价组织芯片的疗效。

Forty-one gastric cancer specimens (20 were differentiated adenocarinoma and 21 undifferentiated) were analyzed in this study. Using Manual tissue arrayer, 2 grafts (2mm in diameter), which showed representative histological feature, were taken out as from each specimen ("donor block"). Forty-eight grafts were put per "recipient block". The graft was lined up in the donor block by histological type and tumor stage. In cutting the recipient block, successfully cut into 3 micro-meter thickness without the grafts in the recipient block being destroyed. It became clear that an array block well reflected the histological structure of donor blocks in tumor cell and gland density when it was compared with donor blocks. In immunohistochemistory, a positive correlation was observed in p-53, Ki-57 and COX-2 expression intensity between donor and microarray (p<0.0005, <0.0005 and <0.0001, respectively). Therefore, diagnostic system by immunohistochemistory using Tissue Microarray was supposed to be useful for human gastric cancerWe are now evaluating the efficacy of Tissue Microarray by analyzing the expression of other proteins, such as VGEF-C, thymidylate synthase, dihydropyrimidine dehydrogenase, thymidine phosphorylase and so on..