Establishment of diagnostic system by immunohistochemistory using Tissue Microarray for human gastric cancer

本研究分析了41例胃癌标本（分化型腺癌20例，未分化型21例）。用手动组织点样器从每个标本中取出2个具有代表性组织学特征的移植物（直径2 mm）（“供体块”）。每个“受体块”放置48个移植物。根据组织学类型和肿瘤分期将移植物在供体块中排列。在切割受体块时，成功地切割成3微米厚，而受体块中的移植物未被破坏。与供体组织块相比，阵列组织块在肿瘤细胞密度和腺体密度方面较好地反映了供体组织块的组织学结构。免疫组化结果显示，p-53、Ki-57和考克斯-2的表达强度在供者和芯片组间均呈正相关（p分别<0.0005、<0.0005和<0.0001）。因此，利用组织芯片进行胃癌的生物化学诊断系统有望成为一种有效的方法。我们目前正在通过分析其他蛋白质的表达，如VGEF-C、胸苷酸合成酶、二氢嘧啶脱氢酶、胸苷磷酸化酶等，来评估组织芯片的诊断效果。

Forty-one gastric cancer specimens (20 were differentiated adenocarinoma and 21 undifferentiated) were analyzed in this study. Using Manual tissue arrayer, 2 grafts (2mm in diameter), which showed representative histological feature, were taken out as from each specimen ("donor block"). Forty-eight grafts were put per "recipient block". The graft was lined up in the donor block by histological type and tumor stage. In cutting the recipient block, successfully cut into 3 micro-meter thickness without the grafts in the recipient block being destroyed. It became clear that an array block well reflected the histological structure of donor blocks in tumor cell and gland density when it was compared with donor blocks. In immunohistochemistory, a positive correlation was observed in p-53, Ki-57 and COX-2 expression intensity between donor and microarray (p<0.0005, <0.0005 and <0.0001, respectively). Therefore, diagnostic system by immunohistochemistory using Tissue Microarray was supposed to be useful for human gastric cancerWe are now evaluating the efficacy of Tissue Microarray by analyzing the expression of other proteins, such as VGEF-C, thymidylate synthase, dihydropyrimidine dehydrogenase, thymidine phosphorylase and so on..