Prevention of ischemic-reperfusion injury during operation by induction of TAP (inhibitor of apoptosis)

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Ischemic-reperfusion (I/R) injury occurs in various situations, including operation, trauma, and shock. We investigated the protective effects of induction of antiapoptotic molecules against hepatic I/R injury.Male WKAH rats were divided into four groups: sham-operation, preconditioning, sham-operation plus hepatic-ischemia, preconditiong plus hepatic-ischemia. Inhibitor of apoptosis protein (IAP), survivin mRNA was induced in WHAH rat liver peaking at 6hrs after ischemic-preconditioning. Expression of survivin protein in the liver was enhanced 6 to 12 hrs after ischemic-preconditioning. To assess the protective effects of survivin, 12 hrs after preconditioning, WKAH rats were subjected to hepatic-ischemia for 30 minutes.In preconditioning plus hepatic-ischemia group, levels of AST, ALT, LDH, and TNF-alpha were reduced compared with sham-operation plus hepatic-ischemia group.Our data suggest that induction of survivin in the liver reduces the hepatic I/R injury.