Prevention of ischemic-reperfusion injury during operation by induction of TAP (inhibitor of apoptosis)

诱导TAP（细胞凋亡抑制剂）预防术中缺血再灌注损伤

• 批准号: 15591421
• 负责人: YAGIHASHI Atsuhito
• 金额: $ 2.11万
• 依托单位: Sapporo Medical University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591421/
• 关键词: survivrn; ischemia-reperfusion injury; apoptosis; preconditioning; hepatic sinusoidal endothelial cell; anti-apoptotic molecule; 虚血再灌流障害; survivin

Ischemic-reperfusion (I/R) injury occurs in various situations, including operation, trauma, and shock. We investigated the protective effects of induction of antiapoptotic molecules against hepatic I/R injury.Male WKAH rats were divided into four groups: sham-operation, preconditioning, sham-operation plus hepatic-ischemia, preconditiong plus hepatic-ischemia. Inhibitor of apoptosis protein (IAP), survivin mRNA was induced in WHAH rat liver peaking at 6hrs after ischemic-preconditioning. Expression of survivin protein in the liver was enhanced 6 to 12 hrs after ischemic-preconditioning. To assess the protective effects of survivin, 12 hrs after preconditioning, WKAH rats were subjected to hepatic-ischemia for 30 minutes.In preconditioning plus hepatic-ischemia group, levels of AST, ALT, LDH, and TNF-alpha were reduced compared with sham-operation plus hepatic-ischemia group.Our data suggest that induction of survivin in the liver reduces the hepatic I/R injury.

缺血再灌注(I/R)损伤发生在手术、创伤、休克等多种情况下。为探讨诱导抗细胞凋亡分子对肝脏I/R损伤的保护作用，将雄性WKAH大鼠分为假手术组、预适应组、假手术+肝缺血组、预适应+肝缺血组。细胞凋亡抑制蛋白(IAP)、Survivin mRNA在WHAH大鼠肝脏中表达，缺血预适应后6h达高峰。缺血预适应后6~12h，肝组织Survivin蛋白表达增强。为评价Survivin的保护作用，在预适应12小时后，WKAH大鼠造成肝脏缺血30分钟。与假手术+肝缺血组相比，预适应+肝缺血组的AST、ALT、LDH和TNF-α水平明显降低。

项目成果

Asanuma K, et al.: "Survivin enhances Fas ligand expression via up-regulation of specificity protein 1-mediated gene transcription in colon cancer cell"J Immunol. 172. 3922-3929 (2004)

Asanuma K 等人：“Survivin 通过上调结肠癌细胞中特异性蛋白 1 介导的基因转录来增强 Fas 配体表达”J Nutrition。

Nakamura M, et al.: "Survivin as a predictor of cis-diamminedichloroplatinum sensitivity in gastric cancer patients."Cancer Sci. 95. 44-51 (2004)

Nakamura M 等人：“生存素作为胃癌患者顺式二氯二氨铂敏感性的预测因子。”Cancer Sci。

Treatment with beta-SQG9 prevents rat hepatic ischemia-reperfusion (I/R) injury.

使用 beta-SQG9 治疗可预防大鼠肝脏缺血再灌注 (I/R) 损伤。

• 期刊: Transplant P (In press)
• 作者: Shima H;et al.
• 通讯作者: et al.

Synthetic sulfonolipids deduced from sulfonoquinovosyl diacylglycerols of sea urchin reduces hepatic ischemia-repefusion injury in rats.

从海胆磺基喹诺酮基二酰基甘油中提取的合成磺脂可减轻大鼠肝缺血再灌注损伤。

• 发表时间: 2004
• 期刊: Transplant P 36
• 作者: Tsuruma T;et al.
• 通讯作者: et al.

Yagihashi A, et al.: "Detection of anti-livin antibody in gastrointestinal cancer patients."Clin Chem. 49. 1206-1208 (2003)

Yagihashi A 等人：“胃肠道癌症患者中抗 Livin 抗体的检测。”Clin Chem。