Establishment of new cold preservation method for small intestinal graft

移植小肠冷藏新方法的建立

• 批准号: 08671463
• 负责人: YAGIHASHI Atsuhito
• 金额: $ 1.34万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671463/
• 关键词: heat-shock protein; ischemia-reperfusion injury; cytokine; small intestineal transplantation; cold presrvation

We examined whether the induction of heat-shock protein (hsp)-73 protects rat small intestine from warm ischemia/reperfusion (I/R) injury and cold preservation injury. To induce the expression of hsp-73 on the small intestine, sodium arsenite (SA) (6mg/kg) was injected to Brown Norway rats 24 hrs before 3hrs-ischemia or 0,10,20,30 hrscold preservation. As the control group, the equal volume of PBS was injected as described above. As for I/R injury, the induction of hsp expression in the small intestine reduces the production of TNF,cytokine-induced neutrophil chemoattractant (CINC) and the magnitude of I/R injury after reperfusion, implying that hsp protects the small intestine from I/R injury. As for cold preservation injury, the induction of hsp expression in the small intestine reduces cold preservation injury. Therefore, 20 hrs-cold preserved grafts were transplanted to syngenic rats. The recipients transplanted hsp expressed small intestinal grafts had significantly longer graft survival than the recipient transplanted no hsp expressed small intestinal grafts.It is appered that the induction of hsp in small intestine is useful for improving I/R and cold preservation injury in small intestinal graft.

我们研究了热休克蛋白（hsp）-73的诱导是否对大鼠小肠热缺血/再灌注（I/R）损伤和冷保存损伤有保护作用。为诱导大鼠小肠hsp-73的表达，在缺血3小时前24小时或缺血保存0、10、20、30小时前给Brown Norway大鼠注射亚砷酸钠（SA）6 mg/kg。作为对照组，如上所述注射等体积的PBS。对于I/R损伤，诱导小肠中的hsp表达减少了再灌注后TNF、马槟榔诱导的中性粒细胞趋化因子（CINC）的产生和I/R损伤的程度，这意味着hsp保护小肠免受I/R损伤。在冷保存损伤方面，诱导小肠组织中hsp表达可减轻冷保存损伤。因此，将20小时冷保存的移植物移植到同系大鼠。移植hsp表达的受体小肠移植物的存活时间明显长于不表达hsp的受体小肠移植物，提示小肠hsp的诱导有利于改善移植物的I/R和冷保存损伤。

项目成果

Tsuruma T: "The inducrion of heat-shock protein-73 reduces the ischemia-reperfusion injury rat sall intestine." Transplant Proc. (In press).

Tsuruma T：“热休克蛋白 73 的诱导减少了大鼠肠道的缺血再灌注损伤。”

Yagihashi A: "Induction of heat-shok protein protects small intestine from preservation injury." Transplant Proc. (In press).

Yagihashi A：“热休克蛋白的诱导可以保护小肠免受保存损伤。”

Sasaki K: "Glutamine protects the morphological structure of small bowel grafts from cold preservation injury." Transplant Proc. 28(3). 1926-1927 (1996)

Sasaki K：“谷氨酰胺可以保护小肠移植物的形态结构免受冷保存损伤。”

Tsuruma T: "The heat-shock protein 70 family reduces ischemia/reperfusion injury in small bowel." Transplant Proc. 28(5). 2629-2630 (1996)

Tsuruma T：“热休克蛋白 70 家族可减少小肠的缺血/再灌注损伤。”

Tsuruma T: "The induction of heat-shock protein-73 reduces the ischemia-reperfusion injury rat sall intestine." Transplant Proc. (In Press).

Tsuruma T：“热休克蛋白 73 的诱导减少了大鼠肠道的缺血再灌注损伤。”