Establishment of new cold preservation method for intestinal graft

移植肠冷藏新方法的建立

• 批准号: 11671258
• 负责人: YAGIHASHI Atsuhito
• 金额: $ 1.41万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671258/
• 关键词: heat-shock protein; ischemia-reperfusion injury; cold presrvation; small intestine; IEC-18; Sodium arsenite; Quercetin; NO; サイトカイン

In this study, we investigate whether the induction of hsp-70 could reduce small intestinal preservation injury in vitro. Intestinal epiethelial cell (IEC)-18 cells were divided two experimental groups. In group 1, the cells were thermally stressed and hsp-70 was induced. In group 2, the cells were cultured at 37℃ without thermal stress. Heat shock was performed in an oven at 43℃ for 30minutes. Cells were then placed in at 37℃ for 2 hours and then preserved at 4℃ in University of Wisconsin (UW) solution for 24 hours. In group 1, the cells were stained with anti-heat shock protein (hsp)-70 antibody 2 hours after thermal stress, but staining was weak in group 2. Magnitude of cold preservation injury was weak in group 1 than in group 2. Survival cell number after cold preservation was higher in group 1 than in group 2. LDH in culture medium 2 hours after cold preservation was higher in group 2 than in group 1. Apoptosis 24 hours after cold preservation was reduced in group 1 when compared with group 2.These results suggest that after inducion of hsp-70 IEC cells become resistant to cold preservation injury, reducing apoptosis.

在本研究中，我们研究了 hsp-70 的诱导是否可以减少体外小肠保存损伤。肠上皮细胞（IEC）-18细胞分为两个实验组。在第 1 组中，细胞受到热应激并诱导 hsp-70。第2组细胞在37℃无热应激条件下培养。热冲击在43℃烘箱中进行30分钟。然后将细胞置于37℃下2小时，然后在4℃下于威斯康星大学(UW)溶液中保存24小时。热应激2小时后，第1组细胞被抗热休克蛋白（hsp）-70抗体染色，第2组染色较弱。第1组冷保存损伤程度较第2组弱。第1组冷保存后存活细胞数高于第2组。冷保存2小时后培养基中LDH高于第1组。冷保存24小时后，第1组细胞凋亡减少1 比较时 与组2相同。这些结果表明，诱导hsp-70 IEC细胞对冷保存损伤具有抵抗力，减少细胞凋亡。

项目成果

Tsuruma T, et al.: "Induction of heat shock protein-70(hsp-70) by intraarterial administration of Geranylgeranylacetone."Transplant P. 32. 1631-1633 (2000)

Tsuruma T 等人：“通过动脉内施用香叶基香叶基丙酮诱导热休克蛋白 70 (hsp-70)。”移植 P. 32. 1631-1633 (2000)

Isobe M, et al.: "Role of inducible nitric oxide synthase on hepatic ischemia and reperfusion injury."Transplant P. 32. 1650-1652 (2000)

Isobe M 等人：“诱导型一氧化氮合酶对肝缺血和再灌注损伤的作用。”移植 P. 32. 1650-1652 (2000)

Tsuruma T: "Induction of heat shock protein-70 (hsp-70) by intaarterial administration of Geranylgeranylacetone."Transplant Proc. 32. 1631-1633 (2000)

Tsuruma T：“通过动脉内施用香叶基香叶基丙酮诱导热休克蛋白 70 (hsp-70)。”移植程序。

Araya J: "Induction of heat shock protein-70 (hsp-70) reduces preservation injury in rat IEC-18 intestinal epithelial cells."Transplant Proc. 32. 1633-1644 (2000)

Araya J：“热休克蛋白 70 (hsp-70) 的诱导可减少大鼠 IEC-18 肠上皮细胞的保存损伤。”Transplant Proc.

Isobe M, et al: "Role of inducible nitric oxide synthase on hepatic ischemia and reperfusion injury."Transplant P. 32. 1650-1652 (2000)

Isobe M 等人：“诱导型一氧化氮合酶对肝缺血和再灌注损伤的作用。”移植 P. 32. 1650-1652 (2000)