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The safe institution of antegrade selective cerebral perfusion in patients with cerebrovaseular diseases, particularly old infarction.

脑血管疾病，特别是陈旧性梗塞患者顺行选择性脑灌注的安全机构。

基本信息

批准号：
15591468
负责人：
KAZUI Teruhisa
金额：
$ 2.24万
依托单位：
Hamamatsu University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Objective :We sought to examine the cerebral protective effects of prophylactically administered Edaravone, in a canine model of old cerebral infarction, undergoing an antegrade selective cerebral perfusion.Methods :A canine model of cerebral infarct was created by injecting a cylindrical silicone embolus in the middle cerebral artery. Animals that developed obvious neurological deficits and had survived for 4 weeks or more were included in the experimental group (n=5). Healthy mongrel dogs were used as control (n=5). Deep hypothermia with antegrade selective cerebral perfusion was performed in the control (non-Edaravone group : group A, Edaravone treated group : group B) and experimental (non-Edaravone group : group C, Edaravone treated group : group D)groups. Serum concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) were measured, and central conduction time and amplitude ratio of somatosensory evoked potential were assessed during the procedure.Result :Both control and experimental groups had similar preoperative parameters. In group C, the serum concentrations of 8-OHdG and MDA, and the central conduction time increased significantly compared with those in group A and B. However the differences between group C and group D with regard to those parameters were not significant. The amplitude ratio in group C significantly decreased compared with that in the other groups.Conclusion :This experiment suggests that the prophylactic administration of Edaravone before antegrade selective cerebral perfusion is likely to provide effective cerebral protection in dogs with old cerebral infarction.

目的：我们试图研究依达拉奉的脑保护作用，在犬模型的陈旧性脑梗死，进行顺行选择性脑perfusion.Methods：犬脑梗死模型，通过注射一个圆柱形的硅胶栓塞在大脑中动脉。将出现明显神经功能缺损并存活4周或更长时间的动物纳入实验组（n=5）。健康杂种犬作为对照（n=5）。对照组（非依达拉奉组：A组，依达拉奉治疗组：B组）和实验组（非依达拉奉组：C组，依达拉奉治疗组：D组）均行深低温顺行选择性脑灌注。结果：对照组与实验组术前各项指标无明显差异，术中测定血清8-羟基脱氧鸟苷（8-OHdG）、丙二醛（MDA）浓度，并测定体感诱发电位（SEP）的中枢传导时间和波幅比。C组血清8-OHdG、MDA浓度及中枢传导时间较A、B组明显增加。而C组和D组在这些参数方面的差异不显著。结论：依达拉奉在选择性脑灌注前预防性给药对陈旧性脑梗死犬有较好的脑保护作用。