Microscopical analysis of the chromium content in the bronchial and lung tissues of chromate workers and genomic changes of the premalignant lesions.

铬酸盐作业工人支气管和肺组织中铬含量的显微分析及癌前病变的基因组变化。

• 批准号: 15591477
• 负责人: KONDO Kazuya
• 金额: $ 1.34万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591477/
• 关键词: chromate lung cancer; premalignant lesions; chromate accumulation; microscopic X-ray fuorescence analyzer; genomic change; microsatellite instability

It is known that chromium is an inhaled carcinogen and an important risk factor in the development of lung carcinoma.METHODS 1 and 2 : The authors used a microscopic X-ray fuorescence analyzer with transmitted X-ray mapping imaging (Horiba, Kyoto, Japan) to measure the accumulation of chromium in 10 resected lung tissue specimens and 90 biopsy specimens from chromate workers. RESULTS 1 and 2 : The maximum chromium accumulation (mean ± standard deviation) in 10 resected lung tissue specimens was 197 ± 238 counts per second (cps)/mili ampere (mA) (range, 4-649 cps/mA). Chromium accumulation was scattered in six tissue specimens and diffuse in one specimen. Chromium accumulation in the proximal bronchi was less than in the bronchioles or subpleural regions of the lung. Chromium accumulation was detectable in 63 (70%) of 90 biopsy specimens, and the mean accumulation was 6.5 ± 9.2 cps/mA (range, 0-46.5 cps/mA). Chromium detected in bronchial tissue specimens was deposited in the bronchial … More stroma but not in the epithelium. The maximum chromium accumulations in dysplastic (n=3), squamous metaplastic (n=10), and normal bronchial epithelia (n=9) in chromate workers and in normal bronchial epithelia (n=3) in non-chromate workers were 20.2 ± 5.4, 18.3 ± 12.2, 13.2 ± 13.4, and 3.0 ± 1.8 cps/mA, respectively. The amount of chromium accumulation significantly increased according to the progression of malignant change of the bronchial epithelium (P=0.003).METHODS 3 : We examined the replication error (RER) and loss of heterozygosity (LOH) in DNA of dysplastic (n=3), squamous metaplastic (n=10) tissues, using six microsatellite markers containing CA repeats : D3S647 (3p23), D3S966 (3p21.3), D3S1289 (3p21.1), D5S346 (5q21-q22), D9S161 (9p21), and TP53 (17p13.1). The RER phenotype was defined as the presence of microsatellite instability (MSI) at two or more loci. RESULTS 3 : Five (78.9%) of 9 premalignant lesions exhibited RER.CONCLUSIONS : Considering genetic aberrations and chromium accumulation in these premalignant lesions is useful for elucidating the process of carcinogenesis in chromium-induced lung carcinoma. Less

已知铬是一种吸入性致癌物质，也是肺癌发生的重要危险因素。方法1和2：作者使用显微X射线荧光分析仪和透射式X射线成像(Horiba，京都，日本)测量了10例肺组织切除标本和90例铬酸盐作业工人的活检标本中铬的积聚。结果1和2：10例肺组织标本的最大铬蓄积量(平均值±标准差)为197±238次/秒(Cps)/毫安(MA)(范围4~649 cps/mA)。铬在6个组织标本中呈分散分布，在1个标本中呈弥散分布。铬在近端支气管处的蓄积少于细支气管处或肺的胸膜下区域。90例活检标本中有63例(70%)可检测到铬蓄积，平均蓄积为6.5±9.2 cps/mA(范围0~46.5 cps/mA)。在支气管组织标本中检测到的铬沉积在支气管…中更多的间质，但不在上皮中。铬酸盐作业工人异型增生(n=3)、鳞状化生(n=10)和正常支气管上皮(n=9)中铬的最大蓄积量分别为20.2±5.4、18.3±12.2、13.2±13.4和3.0±1.8cps/mA。方法：应用含CA重复序列的6个微卫星标记：D3S647(3p23)、D3S966(3p21.3)、D3S1289(3p21.1)、D5S346(5q21-q22)、D9S161(9p21)和TP53(17p13.1)检测了不典型增生(n=3)和鳞状化生(n=10)组织中的复制错误(RER)和杂合性缺失(LOH)。RER表型定义为在两个或多个基因座存在微卫星不稳定性(MSI)。结果：9个癌前病变中有5个(78.9%)出现了RER值。结论：考虑这些癌前病变中的遗传异常和铬蓄积有助于阐明铬诱发肺癌的癌变过程。较少

项目成果

Betulinic acid augments the inhibitory effects of vincristine on growth and lung metastasis of B16F10 melanoma cells in mice.

• DOI: 10.1038/sj.bjc.6601746
• 发表时间: 2004-04-19
• 期刊: British journal of cancer
• 影响因子: 8.8

Microscopical analysis of the chromium content in the chromium-induced malignant and premalignant bronchial lesions of the rat.

铬诱导的大鼠恶性和癌前支气管病变中铬含量的显微镜分析。

• 期刊: Environ Res
• 影响因子: 8.3
• 作者: Kazuya Kondo 他;Kazuya Kondo 他;Kazuya Kondo 他;Kazuya Kondo 他;Kazuya Kondo 他;Kazuya Kondo;Kazuya Kondo他;Kazuya Kondo他;Kazuya Kondo他;Kazuya Kondo;Kazuya Kondo 他
• 通讯作者: Kazuya Kondo 他

WHO histologic classification is a prognostic indicator in thymoma

• DOI: 10.1016/j.athoracsur.2003.07.042
• 发表时间: 2004-04-01
• 期刊: ANNALS OF THORACIC SURGERY
• 影响因子: 4.6
• 作者: Kondo, K;Yoshizawa, K;Monden, Y
• 通讯作者: Monden, Y

Kazuya Kondo他: "Microscopical analysis of the chromium content in the bronchial and lung tissues of chromate workers"Cancer. 98・11. 2420-2429 (2003)

Kazuya Kondo 等：“铬酸盐工人支气管和肺组织中铬含量的显微镜分析”癌症 98・11（2003）。

Tree-dimensional imaging of thoracic diseases with multidetector row CT.

多排排CT胸部疾病的三维成像。

• 发表时间: 2004
• 期刊: J Medical Investigation 51
• 作者: Iida S;Hirota T;Morisaki T;Marumoto T;Hara T;Kuninaka S;Honda S;Kosai K;Kawasuji M;Pallace DC;Saya H.;Kazuya Kondo
• 通讯作者: Kazuya Kondo