Microscopical analysis of the chromium content in the bronchial and lung tissues of chromate workers and genomic changes of the premalignant lesions.

铬酸盐作业工人支气管和肺组织中铬含量的显微分析及癌前病变的基因组变化。

• 批准号: 15591477
• 负责人: KONDO Kazuya
• 金额: $ 1.34万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591477/
• 关键词: chromate lung cancer; premalignant lesions; chromate accumulation; microscopic X-ray fuorescence analyzer; genomic change; microsatellite instability

It is known that chromium is an inhaled carcinogen and an important risk factor in the development of lung carcinoma.METHODS 1 and 2 : The authors used a microscopic X-ray fuorescence analyzer with transmitted X-ray mapping imaging (Horiba, Kyoto, Japan) to measure the accumulation of chromium in 10 resected lung tissue specimens and 90 biopsy specimens from chromate workers. RESULTS 1 and 2 : The maximum chromium accumulation (mean ± standard deviation) in 10 resected lung tissue specimens was 197 ± 238 counts per second (cps)/mili ampere (mA) (range, 4-649 cps/mA). Chromium accumulation was scattered in six tissue specimens and diffuse in one specimen. Chromium accumulation in the proximal bronchi was less than in the bronchioles or subpleural regions of the lung. Chromium accumulation was detectable in 63 (70%) of 90 biopsy specimens, and the mean accumulation was 6.5 ± 9.2 cps/mA (range, 0-46.5 cps/mA). Chromium detected in bronchial tissue specimens was deposited in the bronchial … More stroma but not in the epithelium. The maximum chromium accumulations in dysplastic (n=3), squamous metaplastic (n=10), and normal bronchial epithelia (n=9) in chromate workers and in normal bronchial epithelia (n=3) in non-chromate workers were 20.2 ± 5.4, 18.3 ± 12.2, 13.2 ± 13.4, and 3.0 ± 1.8 cps/mA, respectively. The amount of chromium accumulation significantly increased according to the progression of malignant change of the bronchial epithelium (P=0.003).METHODS 3 : We examined the replication error (RER) and loss of heterozygosity (LOH) in DNA of dysplastic (n=3), squamous metaplastic (n=10) tissues, using six microsatellite markers containing CA repeats : D3S647 (3p23), D3S966 (3p21.3), D3S1289 (3p21.1), D5S346 (5q21-q22), D9S161 (9p21), and TP53 (17p13.1). The RER phenotype was defined as the presence of microsatellite instability (MSI) at two or more loci. RESULTS 3 : Five (78.9%) of 9 premalignant lesions exhibited RER.CONCLUSIONS : Considering genetic aberrations and chromium accumulation in these premalignant lesions is useful for elucidating the process of carcinogenesis in chromium-induced lung carcinoma. Less

众所周知，铬是一种遗传的致癌物，也是肺癌发展的重要危险因素。方法1和2：作者使用了微观X射线燃料分析仪，其X射线映射成像（Horiba，Horiba，kyoto，Japan，日本）从10个chromium consement and chrong specome speclime and consement and consement and consement and 90衡量。结果1和2：10切除的肺组织标本中的最大铬积聚（平均值±标准偏差）为197±238次计数每秒（CPS）/MILI AMPERE（MA）（范围为4-649 CPS/MA）。铬积聚散射在六个组织样品中，并在一个标本中弥漫。近端支气管中的铬积聚小于肺的细支气管或胸膜下区域。在90个活检标本中的63个（70％）中检测到铬的积累，平均积累为6.5±9.2 cps/mA（范围为0-46.5 cps/ma）。在支气管组织样品中检测到的铬被沉积在支气管中……更多的基质，但在上皮中没有。发育不良（n = 3）的最大铬积聚，鳞状化生（n = 10）和铬酸盐工人中的正常支气管上皮（n = 9），在非染色体工人中的正常支气管上皮（n = 3）中，非染色器工人的最大铬含量为20.2±5.4，18.3±5.4，18.3±12.2.2.2，13.2，13.2，13.2，13.2; 分别。根据支气管上皮的恶性变化的进展（p = 0.003）。方法3：我们检查了复制误差（RER）和杂合性（loh）的DNA在不倍倍症（n = 3），sm亵sm骨质量（n = 10）中，使用杂合性（loh），使用了复制误差（RER）和杂合性（loh），使用六微小（n = 10），使用杂合性（loh），使用六微小（n = 10） D3S647（3P23），D3S966（3P21.3），D3S1289（3P21.1），D5S346（5Q21-Q22），D9S161（9P21）和TP53（17p13.1）。 RER表型被定义为在两个或多个基因座处的微卫星不稳定性（MSI）的存在。结果3：9个预抗病变中的五个（78.9％）暴露于RER.CONCLUSIONS：考虑到这些前病变中的遗传像差和铬积累，有助于阐明铬诱导的肺癌中的致癌过程。较少的

项目成果

Kazuya Kondo他: "Microscopical analysis of the chromium content in the bronchial and lung tissues of chromate workers"Cancer. 98・11. 2420-2429 (2003)

Kazuya Kondo 等：“铬酸盐工人支气管和肺组织中铬含量的显微镜分析”癌症 98・11（2003）。

Betulinic acid augments the inhibitory effects of vincristine on growth and lung metastasis of B16F10 melanoma cells in mice.

• DOI: 10.1038/sj.bjc.6601746
• 发表时间: 2004-04-19
• 期刊: British journal of cancer
• 影响因子: 8.8

Microscopical analysis of the chromium content in the chromium-induced malignant and premalignant bronchial lesions of the rat.

铬诱导的大鼠恶性和癌前支气管病变中铬含量的显微镜分析。

• 期刊: Environ Res
• 影响因子: 8.3
• 作者: Kazuya Kondo 他;Kazuya Kondo 他;Kazuya Kondo 他;Kazuya Kondo 他;Kazuya Kondo 他;Kazuya Kondo;Kazuya Kondo他;Kazuya Kondo他;Kazuya Kondo他;Kazuya Kondo;Kazuya Kondo 他
• 通讯作者: Kazuya Kondo 他

Establishment of patient-like SCID mouse model by orthotopically implanting primary cultured cells from surgically-resected lung cancer tissues.

通过原位植入手术切除的肺癌组织的原代培养细胞建立类患者 SCID 小鼠模型。

• 发表时间: 2003
• 期刊: Oncology reports 10
• 作者: Kazuya Kondo 他;Kazuya Kondo 他;Kazuya Kondo 他;Kazuya Kondo 他;Kazuya Kondo 他;Kazuya Kondo;Kazuya Kondo他;Kazuya Kondo他;Kazuya Kondo他;Kazuya Kondo
• 通讯作者: Kazuya Kondo

Tree-dimensional imaging of thoracic diseases with multidetector row CT.

多排排CT胸部疾病的三维成像。

• 发表时间: 2004
• 期刊: J Medical Investigation 51
• 作者: Iida S;Hirota T;Morisaki T;Marumoto T;Hara T;Kuninaka S;Honda S;Kosai K;Kawasuji M;Pallace DC;Saya H.;Kazuya Kondo
• 通讯作者: Kazuya Kondo