Renodeling treatment for cerebral aneurysms

1)评价光交联壳聚糖作为动脉瘤栓塞剂的可行性。用3只日本大白兔制作了3个实验性动脉瘤模型。所有动脉瘤均用壳聚糖水凝胶填塞。分析栓塞术后第2、7、30天的组织病理学资料。植入两天后，观察到应用的壳聚糖水凝胶周围有无组织的凝块和轻微的炎症。7天后，壳聚糖减少，出现炎症反应。30d时，瘤腔大部分已被炎性细胞取代，未见残留的壳聚糖。壳聚糖栓塞术后未发生过敏反应等严重并发症。这些结果表明，光交联壳聚糖可能是一种候选的血管内治疗脑动脉瘤的材料。2)用高频交变磁场(AMF…)填充玻璃模型动脉瘤此外，还研究了铂的重量与温度升高之间的关系，以及流过模型的水流量与温度升高之间的关系。其次，对不同温度下犬血液的活化凝血时间(ACT)进行了研究。最后，使用静脉补片在犬颈动脉制造侧壁动脉瘤，用铂金弹簧圈填充。观察AMF后的体温变化和血管造影变化。在玻璃模型中，铂的重量与温度的升高相关，流量与温度的升高呈明显的负对数关系。血样温度升高有缩短ACT的趋势。在犬颈动脉动脉瘤中，证实了动脉瘤内温度的升高，并发现充分的温度升高促进了血管造影显示的残余间隙的血栓形成。局部热疗可有效地完成弹簧圈栓塞后动脉瘤的腔内闭塞。对于破裂的动脉瘤可能特别有用，以防止早期复发。3)将Polturethan涂层支架放置在三只犬的颈动脉内导致完全血栓形成。将自体血管内皮细胞植入聚氨基甲酸乙酯薄膜，以减少血栓形成。细胞培养成功，膜上附着的内皮细胞层即使在酸蚀、旋转、流动等各种机械应力作用下仍未脱落。较少

1)The feasibility of photocrosslinkable chitosan as an embolization material for aneurysms was evaluated. Three experimental aneurysms were created in three Japanese white rabbits. All of the aneurysms were packed with chitosan hydrogel. Histopathologic data were analyzed on two, seven, and 30 days after embolization. Unorganized clots and minimal inflammation around the applied chitosan hydrogel were observed two days after implantation. After seven days, the chitosan was reduced and inflammatory response appeared. At 30 days, most of the aneurysm lumen was replaced with inflammatory cells, and the remaining chitosan was not observed. Severe complications such as anaphylaxis did not occur after the embolization with the chitosan. These results suggest that photocrosslinkable chitosan might be a candidate for an embolization material for endovascular treatment of cerebral aneurysms.2)Glass model aneurysms packed with coils were subjected to high-frequency altemating magnetic fields(AMF … More ) to investigate the correlation between weight of platinum and temperature elevation and the correlation between flow rates of water through the model and temperature elevation. Next, activated coagulation time(ACT) of blood obtained from dogs was studies at various temperatures. Finally, side-wall aneurysms created in the canine carotid artery using a venous patch were packed with platinum coils. Change in temperature and angiographic change were investigated after AMF. In the glass model, the weight of platinum was correlated with elevation of temperature, and a negative logarithmic correlation was evident between flow rate and elevation of temperature. Elevation of blood sample temperature tended to shorten ACT. In Canine carotid aneurysms, elevation of intra-aneurysmal temperature was confirmed and sufficient elevation of temperature was found to promote angiographically evident thrombogenesis of the remnant space after AMF application. Local hyperthermia may be useful in completing luminal obliteration of aneurysms after coil embolization. It may particularly usuful for ruptured aneurysms to prevent the early rerupture.3)Polturethan-coated stent was pplaced in three canine carotid artery resulted in complete thrombosis. Autologus endothelium was implanted on the polyurethan film to reduce the thrombogenisity. Cell culture was succeeded and attached endothelium layer on the film was found not to come off even after loading with various machnical stress like as steretch, rotation and the flow. Less