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Bone allograft used with autologous bone mallow cell culture

与自体骨髓细胞培养一起使用的同种异体骨移植

基本信息

批准号：
15591582
负责人：
KAJI Yoshio
金额：
$ 2.05万
依托单位：
Kagawa university.
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591582/
关键词：
bone graft allograft BMP bisphoshonate 細胞培養ビスフォスオフォネート

项目摘要

Folloing results had been obtained during the term of project.Two-mm-thick bone discs were collected from femoral mid-shaft of donor rats, and BMP and/or bisphosphonate were added into the bone discs. These discs were transplanted into paravertebral muscles of recipient rats. One month later, the transplanted discs were collected and bone formation and bone resorption of these discs were evaluated. Although almost no bone formation and bone resorption was observed in the discs of control group, BMP increased bone formation of the bone disc. When bisphosphonate was added with BMP, bone resorption was also reduced. After this experiment we performed following bone graft experiment. Ten-mm-long bone were collected from femoral mid-shaft of donor rats, and BMP and/or bisphosphonate were added into these graft bones. These bones were transplanted into bone defects of femoral shaft of recipients rats. BMP raised bone formation of transplanted bone and bone union rate. When bisphosphonate was added with BMP, bone resorption was also reduced. These results suggested that addition of BMP and bisphosphonate enable bigger bone allograft.We are now trying those experiments in which bone mallow cells of recipients rat are cultured in the bone discs of donor rats in the presence of BMP and bisphosphonate before the transprantation.

取供体大鼠股骨中段2 mm厚的骨盘，在其中加入骨形态发生蛋白和/或双磷酸盐。将这些椎间盘移植到受体大鼠的椎旁肌肉中。1个月后，收集移植的间盘，评价其成骨和骨吸收情况。虽然对照组的骨盘几乎没有骨形成和骨吸收，但BMP促进了骨盘的骨形成。当双膦酸盐与骨形态发生蛋白联合使用时，骨吸收也有所减少。实验结束后，进行后续的植骨实验。取供体大鼠股骨中段10 mm长的骨，植入骨形态发生蛋白和/或双磷酸盐。将这些骨移植到受体大鼠股骨干的骨缺损处。BMP可提高移植骨的成骨能力和骨愈合率。当双膦酸盐与骨形态发生蛋白联合使用时，骨吸收也有所减少。这些结果表明，添加BMP和双磷酸盐可以实现更大的同种异体骨移植。我们正在尝试在移植前在供体大鼠的骨盘中加入BMP和双膦酸盐培养受体大鼠的骨葵细胞的实验。