The presence of cell-free fetal DNA in the serum from non-pregnant women with thyroid disease

患有甲状腺疾病的非孕妇血清中存在无细胞胎儿 DNA

• 批准号: 15591751
• 负责人: SAMURA Osamu
• 金额: $ 1.92万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591751/
• 关键词: FISH; Real Time PCR; Microchimerism; Fetal DNA in maternal blood; SRY; Microshimerism; Real time PCR法

Microchimerism is the state that low-level donor cells or progenitor cells maintain in a host. Recently, microchimerism has been considered as the possible pathogenesis of some diseases, including auto immune diseases. Our aim was to examine whether or not cell-free fetal DNA existed in the serum collected from non-pregnant women who were suffering from thyroid disease. After obtaining written informed consent, we took peripheral blood samples from 99 non-pregnant women suffering from thyroid disease. For the control group, we recruited 71 non-pregnant women without either thyroid disease or auto immune diseases. DNA was extracted from the serum. Then, we performed a real-time quantitative polymerase chain reaction (PCR) using the SRY sequence as a marker of fetal microchimerism. We detected the SRY sequence in 6 of 99 patients (6.1%). The longest period after the birth of the youngest son was 56 years. On the other hand, the SRY sequence was not detectable in the control group (0%). This is the first report to examine microchimerism in the serum from patients with thyroid disease. We detected cell-free fetal DNA in serum specimens taken from non-pregnant women suffering from thyroid disease. Our findings suggest that fetal microchimerism is a possible pathogenesis of thyroid diseases.

微嵌合体是指低水平的供体细胞或祖细胞在宿主体内保持的状态。近年来，微嵌合体被认为是包括自身免疫性疾病在内的一些疾病的可能发病机制。我们的目的是检查从患有甲状腺疾病的非怀孕妇女收集的血清中是否存在无细胞胎儿DNA。在获得书面知情同意后，我们采集了99名患有甲状腺疾病的非妊娠妇女的外周血样本。对于对照组，我们招募了71名既没有甲状腺疾病也没有自身免疫性疾病的非怀孕妇女。从血清中提取DNA。然后，我们进行了实时定量聚合酶链式反应(PCR)，使用SRY序列作为胎儿微嵌合体的标志。我们在99例患者中有6例检测到SRY序列(6.1%)。最小的儿子出生后最长的时期是56岁。另一方面，对照组(0%)未检测到SRY序列。这是第一份检测甲状腺疾病患者血清中微嵌合体的报告。我们从患有甲状腺疾病的非妊娠妇女的血清样本中检测到无细胞胎儿DNA。我们的发现提示，胎儿微嵌合体可能是甲状腺疾病的一种发病机制。

项目成果

Increased plasma mRNAs of placenta-specific 1 (PLAC1) and glial cells-missing 1 (GCM1) in mothers with pre-eclampsia.

• 发表时间: 2006-03
• 期刊: Hiroshima journal of medical sciences
• 作者: N. Fujito;O. Samura;N. Miharu;M. Tanigawa;Maki Hyodo;Y. Kudo

Molar DNA in maternal serum in a case of 46,XY heterozygous complete hydatidiform mole coexisiting with a 46,XX twin live fetus

46,XY 杂合完全葡萄胎与 46,XX 双胞胎活胎共存一例母体血清中摩尔 DNA

• 发表时间: 2005
• 期刊: Clinical Chemistry 51(3)
• 作者: Shirakawa;T.;Hamada;K.;Zhang;Z.;Okada;H.;Tagawa;T.;Kamidono;S.;Kawabata;M.;Gotoh;A.;Maki Hyodo et al.
• 通讯作者: Maki Hyodo et al.

女性における甲状腺疾患と非妊娠時の血中胎児成分との関連

非孕期女性甲状腺疾病与胎儿血液成分的关系

• 发表时间: 2006
• 期刊: 日本産科婦人科学会雑誌 58・2
• 作者: Shih HC;(他6名、2番目);Naoya Fujito et al.;谷川 美穂
• 通讯作者: 谷川 美穂

Molar DNA in maternal serum in a case of 46, XY heterozygous complete hydatidiform mole coexisiting with a 46, XX twin live fetus

46, XY 杂合完全葡萄胎与 46, XX 双胞胎活胎共存一例母体血清中摩尔 DNA

• 发表时间: 2005
• 期刊: Clinical Chemistry 51(3)
• 作者: Saito;M.;Nakagawa;K.;Hamada;K.;H i rose;S.;Harada;H.;Kohno;S.;Nagato;S.;Ohnishi;T.;Maki Hyodo et al.
• 通讯作者: Maki Hyodo et al.