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Ultrastructural enzymehistochemical and cell-biological analysis of the preterm dilivery -relationship between fetal cells and preterm delivery-

早产的超微结构酶组织化学和细胞生物学分析-胎儿细胞与早产之间的关系-

基本信息

批准号：
15591775
负责人：
MATSUBARA Shigeki
金额：
$ 1.15万
依托单位：
JICHI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591775/
关键词：
amnion chorion placenta macrophage enzymehistochemistry preterm delivery leukocyte phagocytosis 組織化学

项目摘要

1.We characterized the morphology of the human amniotic epithelial cells in term pregnancies. In the chemically fixed amnion, there were two types of cells with different degree of cytoplasmic darkness. However, there were no differences in the number and the shape of Golgi apparatus and mitochondria between the cells. The distribution pattern and the intensity of the enzyme detected by the enzymehistochemistry did not differ between cells, either. Freeze-substitution fixation abolished the differences of the cytoplasmic darkness between cells. We concluded that human amniotic epithelial cells consisted of the homogeneous cell population as far as the morphological features were concerned.2.In the chorioamnion from women with preterm delivery there were abundant polymorphonuclear leukocytes. These leukocytes exhibited morphological features characteristic of phagocytosing leukocytes, ie. : appearance of phagocytotic vacuole with myeloperoxidase on the phagocytotic membrane. These activated leukocytes accumulated just in front of the basement membrane of the chorioamnion. We concluded that in the chorioamnion of the preterm delivery maternal leukocytes accumulated at the basement membrane of this tissue and they phagocytosed various cell debris in this membrane. The phenomenon observed here may have close association with the development of the preterm delivery.3.We developed dual staining for acid phosphatase and glucose-6-phosphate dehydrogenase histochemistry at an ultrastructural level. We applied this technique to the fetal placental macrophages in preterm delivery. The macrophages with positive staining for glucose-6-phosphate dehydrogenase were defined as activated macrophages. The number of activated fetal placental macrophages were significantly larger in the placenta with preterm delivery than that with normal term delivery. We concluded that fetal placental macrophages had close association with the initiation of the preterm delivery.

1.对足月妊娠人羊膜上皮细胞进行了形态学观察。在化学固定的羊膜中，有两种类型的细胞具有不同程度的胞质暗度。而高尔基体和线粒体的数量和形状在细胞间无差异。酶组织化学检测到的酶的分布模式和强度在细胞之间也没有差异。冷冻置换固定消除了细胞间的胞质暗度差异。结论：人羊膜上皮细胞在形态学特征上是一个均一的细胞群。2.早产妇女绒毛膜羊膜中含有丰富的多形核白细胞。这些白细胞具有吞噬白细胞的形态特征，即：吞噬细胞膜上出现吞噬空泡和髓过氧化物酶。这些活化的白细胞聚集在绒毛膜羊膜的基底膜前面。我们的结论是，在绒毛膜羊膜的早产母体白细胞积累在这个组织的基底膜，他们吞噬各种细胞碎片在这个膜。本实验观察到的现象可能与早产的发生有密切的关系。3.在超微结构水平上，我们建立了酸性磷酸酶和葡萄糖-6-磷酸脱氢酶组织化学双重染色。我们将此技术应用于早产胎儿胎盘巨噬细胞。将葡萄糖-6-磷酸脱氢酶染色阳性的巨噬细胞定义为活化的巨噬细胞。早产胎盘中激活的胎儿胎盘巨噬细胞数量明显高于正常足月分娩胎盘。结论：胎儿胎盘巨噬细胞与早产的发生密切相关。