Topological analysis of GPI-80 in human neutrophils

人中性粒细胞中 GPI-80 的拓扑分析

• 批准号: 13670022
• 负责人: MATSUBARA Shigeki
• 金额: $ 2.05万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670022/
• 关键词: human neutrophils; GPI-80; GPI-anchored protein; CD18; intracellular granules; immunocytochemistry; cell biology; 細胞内穎粒

GPI-80 is a novel GPI-anchored protein on human phagocytes that may regulate leukocyte adherence, locomotion, and extravasation. A unique intracellular compartment that contains GPI-80 is distinct from the previously characterized azurophil and specific granules. The GPI-80-containing compartments rapidly undergo a dramatic reorganization upon cell stimulation with a chemoattractant. By high-resolution immunofluorescence microscopy using ultrathin cryosections, after fMLP-stimulation, some GPI-80 molecules were co localized with beta 2 integrin molecules (CD18) on the cell surface. These results suggest that GPI-80 is physically associated with beta 2 integrin in human neutrophils.

GPI-80是一种新的GPI锚定蛋白，可调节人吞噬细胞的粘附，运动和外渗。一个独特的细胞内室，含有GPI-80是不同于以前的特点嗜天青细胞和特定的颗粒。含有GPI-80的隔室在用化学引诱物刺激细胞后迅速经历戏剧性的重组。通过高分辨率免疫荧光显微镜使用冷冻切片，在fMLP刺激后，一些GPI-80分子与β 2整合素分子（CD 18）共定位在细胞表面上。这些结果表明，GPI-80与人中性粒细胞中的β 2整联蛋白物理相关。

项目成果

Yamada, Tetsuo: "Changes in the number of polymorphonuclear leukocytes and concentrations of IL-8 and granulocyte elastase in the vaginas of normal pregnant women"Am J Reprod Immunol. 47. 98-103 (2002)

Yamada, Tetsuo：“正常孕妇阴道中多形核白细胞数量以及 IL-8 和粒细胞弹性蛋白酶浓度的变化”Am J Reprod Immunol。

Matsubara, Shigeki: "Immuno-electron microscopic localization of endothelial nitric oxide synthase in human placental terminal villous trophoblasts-normal and pre-eclamptic pregnancy"Placenta. 22. 782-786 (2001)

Matsubara，Shigeki：“人胎盘终末绒毛滋养细胞中内皮一氧化氮合酶的免疫电子显微镜定位 - 正常和先兆子痫妊娠”胎盘。