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Cytochemical analysis of the mouse spermatogenesis

小鼠精子发生的细胞化学分析

基本信息

批准号：
17591754
负责人：
MATSUBARA Shigeki
金额：
$ 1.09万
依托单位：
Jichi Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591754/
关键词：
spermatogenesis differentiation TEX101 apoptosis testis epididymis seminal duct immuno-histochemistry 発生・分化精子

项目摘要

TEX101 is the 38kDa mouse testicular protein and has been registered to Mouse Genome Informatics (MGI) database. TEX101 has been given an ID number as 1930791. In this study, we determined the localization of TEX101 and TEX101 mRNA in adult mouse testis. At the same time we determined TEX101 localization in epididymis and was deferens of adult mouse. For the detection of TEX101, we used immunohistochemistry. For the detection of TEX101 mRNA, we employed the in situ hybridization.In mouse testis, TEX101 was observed in various stages of spermatocytes, all stages of spermatids (step 1-16), and sperm. Spermatogonia showed no TEX101 immunolocallization. TEX101 mRNA was observed both in spermatocytes and step 1-9 spermatids in testis. However, TEX101 mRNA was not observed in step 10-16 spermatids and sperm. Spermatogonia also showed no TEX101 mRNA signaling. Thus, both step 10-16 spermatids and sperm in testis showed positive TEX101 but negative TEX101 mRNA signalings. This indicates that in step 10-16 spermatids and sperm, although these cells showed positive immunoreactivity for TEX101, TEX101 is not being synthesized in these cells in de novo. Rather, TEX101 protein may remain in the cell surface on these cells after synthesis.Sperm in the epididymis and was deferens did not show TEX101 immunoreativity, although sperm in testis showed intense staining for TEX101. Thus, sperm may loose TEX101 protein while sperm move from the testis to epididymis, and finally to was deferens. TEX101 was shed from epididymal sperm during the sperm transfer from testis to epididymis.All these indicate that TEX101 is expressed in a stage-specific manner in spermatids, and this protein may have something to do with the capacitation of the sperm.

TEX101是一种38 kDa的小鼠睾丸蛋白，已在小鼠基因组信息学数据库中注册。TEX101的ID号为1930791。在本研究中，我们确定了TEX101和TEX101在成年小鼠睾丸中的定位。同时对TEX101在成年小鼠附精和输精管中的定位进行了研究。TEX101的检测采用免疫组织化学方法。为了检测TEX101的mRNA，我们采用原位杂交的方法。在小鼠的睾丸中，TEX101在精母细胞的不同阶段、所有阶段的精子细胞(步骤1-16)和精子中都有表达。精原细胞未见TEX101免疫定位。TEX101在精母细胞和睾丸中的STEP 1-9精子细胞中均有表达。然而，在第10-16步精子细胞和精子中未观察到TEX101基因的表达。精原细胞也未显示TEX101信使。因此，Step 10-16精子细胞和睾丸中的精子均显示TEX101阳性信号，而TEX101 mRNA信号为阴性。这表明，在第10-16步精子细胞和精子中，虽然这些细胞对TEX101呈阳性免疫反应，但在从头开始这些细胞中不合成TEX101。而在这些细胞合成后，TEX101蛋白可能仍留在细胞表面。虽然睾丸中的精子显示出强烈的TEX101免疫反应，但附睾和输精管中的精子不显示TEX101免疫反应性。因此，当精子从睾丸运动到附睾，最后到达输精管时，精子可能会释放TEX101蛋白。TEX101是在精子从睾丸向附睾转移的过程中从附睾精子中脱落出来的，这表明TEX101在精子细胞中以阶段特异性的方式表达，该蛋白可能与精子的获能有关。