Establishment of Experimental Autoimmune Inner Ear Disease Model Using New Immunological Procedures

用(1)II型胶原致敏小鼠，(2)CpG基序和II型胶原致敏小鼠，或(3)葡萄球菌肠毒素B（超抗原）和II型胶原致敏小鼠，并通过听脑干反应检测听力水平。各组患者均未见明显听力损失。在我们的实验中，II型胶原蛋白的致敏作用可能不够强，因为每组的关节炎都很轻微。(4)注射超抗原或(5)Freun' s完全佐剂（FCA）后，检测MRL/MpJ-lpr/lpr （MRL/lpr）小鼠的听力水平。部分MRL/lpr小鼠在FCA致敏后出现听力损伤。苏木精染色和伊红染色未见MRL/lpr小鼠耳蜗明显病理改变。对MRL/lpr小鼠血管纹内皮进行IgG和ICAM - 1免疫染色。然而，这些发现不仅出现在注射FCA后出现听力障碍的小鼠身上，也出现在注射FCA或生理盐水后没有听力损失的动物身上。进一步研究血管纹的免疫反应对了解自身免疫性内耳疾病的病理生理有重要意义。

Mice were sensitized with (1)type II collagen, (2)CpG motif and type II collagen, or (3)Staphylococcal enterotoxin B (superantigen) and type II collagen, and examined hearing level by auditory brainstem responses. No obvious hearing loss was detected in each group. Sensitization of type II collagen might not be strong enough in our experiments because arthritis seen in each group was slight.Hearing level of MRL/MpJ-lpr/lpr (MRL/lpr) mice was examined after they were injected with (4)superantigen or (5)Freun' s complete adjuvant (FCA). Some MRL/lpr mice showed impaired hearing after the sensitization with FCA.Hematoxylin and eosin staining could not reveal obvious pathologic changes in the cochleas of the MRL/lpr mice with hearing loss. Immunostaining for IgG and ICAM 1 was observed on the endothelia of the stria vascularis vessels of the MRL/lpr mice. These findings, however, were seen not only in the mice with hearing disturbance after FCA injection, but also in the animals without hearing loss after the injection with FCA or saline. Further studies of the immune responses on the stria vascularis vessels might be important to understand the pathophysiology of the autoimmune inner ear diseases.