Determination of treatment modalities for diabetic retinopathy and diabetic maculopathy -Strategic approach using molecular and cellular biological methods-

确定糖尿病视网膜病变和糖尿病黄斑病变的治疗方式 -使用分子和细胞生物学方法的策略方法-

基本信息

批准号：
15591841
负责人：
YAMASHITA Hidetoshi
金额：
$ 2.24万
依托单位：
Yamagata University Faculty of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591841/
关键词：
Diabetic retinopathy VEGF IL-6 oxidative stress 8-OHdG NOx severity of diabetic retinopathy SNPs 重症度判定 OCT 遺伝子 SNPs DGKs

项目摘要

Various factors are involved in the pathogenesis of diabetic retinopathy and diabetic maculopathy (macular edema). The candidates of the risk factors are factors related to oxidative stress and retinal angiogenesis. As the first step, to investigate the candidate molecules to play important roles in diabetic retinopathy, the expression levels of the bio-marker of cell damage due to oxidative stress ((8-OHdG and NOx) and cytokines (VEGF and IL-6) were measured in the vitreous humor obtained from the diabetic eyes after securing the written permission. This study was approved by the ethical committee of Yamagata University Faculty of Medicine. This study has revealed that the diabetic retinopathy and the diabetic maculopathy are closely related to the oxidative stress and VEGF, IL-6. As the second step, to investigate the molecular pathogenesis of diabetic retinopathy by focusing the retinal damage to retinal neurons, the intracellular signal transduction of hyperglycemia, including abno … More rmal metabolic pathways and cytokine expression acceleration, has been investigated using the in vitro systems. This study has revealed that diacylglycerol activates protein kinase C, which increases VEGF expression in retinal cells.Taken together, VEGF is one of the main factors to be involved in the pathogenesis of diabetic retinopathy and maculopathy. One of the single nucleotide polymorphisms (SNPs) in the VEGF gene was investigated. The frequencies of VEGF +813 C-T (CC, CT, TT) were determined in DNA from 108 subjects (non DM=12 subjects, DM=96 subjects). The frequencies of the VEGF +813 C_T polymorphisms were similar in both DM and non DM groups. The severity of retinopathy was not related to VEGF +813 polymorphism. The results of this study have revealed that the expression levels of cytokines and oxidative stress related factors, not the genetic factor were related to diabetic retinopathy and maculopathy. It is mandatory to search for the more useful factors relevant to diabetic retinopathy and maculopathy to set up the system to determine the treatment modalities for diabetic retinopathy and maculopathy. Less

糖尿病性视网膜病和糖尿病性糖果病的发病机理涉及各种因素（黄斑水肿）。危险因素的候选者是与氧化应激和视网膜血管生成有关的因素。作为第一步，要调查候选分子在糖尿病性视网膜病变中起重要作用，将氧化压力（（（8-OHDG和NOX）和细胞因子和细胞因子（VEGF和IL-6）造成的细胞损害的生物标志物的表达水平（VEGF和IL-6）是通过在糖尿病上估算的。这项研究表明，糖尿病性视网膜病变和糖尿病性棒状病与氧化应激和VEGF紧密相关，IL-6是第二步。使用体外系统研究了途径和细胞因子表达加速度。研究的频率在108位受试者的DNA中（CC，CT，TT）（非DM = 12个受试者，DM = 96个受试者）在VEGF +813 C_T的频率中这项研究表明，细胞因子和氧化应激相关因素的表达水平，而不是遗传因素与糖尿病性视网膜病变和baculopathy有关