Immunological analysis of dendritic cell-osteopontin system in the remodeling of serious ocular allergy

春性角膜结膜炎（VKC）和特应性角膜结膜炎（VKC）患者产生il -4的结膜T细胞频率明显高于变应性结膜炎（AC）患者。AC、AKC、VKC患者结膜CD4+ T细胞产生il -4的频率明显高于同病患者外周血CD4+ T细胞。此外，与变应性结膜疾病患者的PBMC相比，结膜中p选择素配体表达il -4产生T细胞的频率增加。ACD患者外周血产生il -4的T细胞频率明显高于正常对照组。采用ELISA（酶联免疫吸附法）检测49例ACD患者（过敏性结膜炎14例、特应性角膜结膜炎14例、春性角膜结膜炎21例）泪液中细胞因子、白细胞介素-2（IL-2）、干扰素γ (IFN-γ)、IL-4、IL-5、IL-6、IL-8、IL-13水平。对细胞因子的相关性进行统计学分析。IFN-g与IL-13、IL-4与IL-13、IL-5与IL-6、IL-8与IL-13之间存在显著相关性。这些结果提示，产生il -4的T细胞浸润到结膜中，在变应性结膜疾病的免疫发病机制中起重要作用，p -选择素配体参与T细胞进入过敏性炎症部位，泪液细胞因子之间的相关性呈现多种模式，提示细胞因子的复杂性和冗余性。

The frequency of IL-4-producing conjunctival T cells in patients with vernal keratoconjunctivitis (VKC) and atopic keratoconjunctivitis (VKC) were significantly higher than that in patients with allergic conjunctivitis (AC). The frequencies of IL-4-producing conjunctival CD4+ T cells of the patients with AC, AKC, or VKC were significantly higher than those of peripheral blood CD4+ T cells of the same disease. Further, increased frequency of P-selectin ligand expressing IL-4-producing T cells was observed in conjunctiva compared to that in PBMC of the patients with allergic conjunctival diseases. The frequencies of IL-4-producing peripheral blood T cells in the patients with ACD were sig-nificantly higher than that in normal controls. Tear levels of cytokines, interleukin-2(IL-2), interferon gamma (IFN-γ), IL-4, IL-5, IL-6, IL-8 and IL-13, in 49 patients with ACD (allergic conjunctivitis 14 cases, atopic keratoconjunctivitis 14 cases and vernal keratoconjunctivitis 21 cases) were measured with ELISA (enzyme-linked immunosorbent assay) method. Correlation between cytokines were statistically analyzed. Significant correlations were observed between IFN-g and IL-13, IL-4 and IL-13, IL-5 and IL-6, and IL-8 and IL-13. These results suggest that IL-4-producing T cells that have infiltrated into the conjunctiva play an important role in the immunopathogenesis of allergic conjunctival diseases, and P-selectin ligand is involved in the entry of T cells into allergic inflammatory site and correlation between tear cytokines show various patterns suggesting the complexity and redundancy of cytokines.