Arginine effect on the absorption and motor functions of the residual ileum after massive small intestinal resection

【目的】建立一种能同时评价精氨酸在大鼠小肠吸收和运动功能的实验系统，为精氨酸在大鼠小肠中的应用提供实验依据。[方法]1）取4周龄雄性SD大鼠，切除近端小肠90%，在回盲瓣近端5cm处吻合。对接受该手术的大鼠和具有正常小肠的大鼠施用精氨酸或甘氨酸。以这种方式制备总共四组。2）使用遥测系统分析肠运动。为了记录残留回肠的运动功能，在腹腔内给予戊巴比妥后，使用6-0尼龙将4 mm的力传感器（FT）缝合到吻合部位远端2 cm的回肠薄而脆弱的壁上。在给FT前，给予硫酸阿托品0.1mg/kg， 关于我们 皮下注射。使用小的发射器（用于小鼠），使用5-0丝固定在腹腔内的腹壁上，从包埋FT后3天起重复记录环行肌的收缩。在90%近端小肠切除术后3周和6周，每小时计算禁食12或24小时前后收缩波的积分值。3）在手术后3周和6周，通过腹膜内给予50 mg D-木糖并使用10小时尿样基于尿排泄计算吸收率来评估吸收功能。[结果]1）精氨酸餐后每小时收缩波积分值小于空腹前，而甘氨酸餐后每小时收缩波积分值与空腹前无差异。在禁食期间出现的大收缩运动的数量和持续时间被认为相当于迁移性肌电（或运动）复合体（MMC），与精氨酸给药无关。2）在术后3周和6周测量吸收功能。虽然精氨酸给药在术后3周和4周改善了吸收，但在术后6周没有观察到进一步改善。[结论]精氨酸仅在术后3周内改善了短小肠模型的吸收功能。因此，需要进一步的研究，以确定精氨酸对肠道运动的影响。少

[Objective]The present study was conducted, using our short-small intestine model that is suitable for investigating the importance of arginine in vivo, in order to establish an experiment system that simultaneously assesses the absorption and motor functions of the residual ileum.[Methods]1)Using 4-week-old male SD rats, 90% of the proximal small intestine was excised and the remaining intestine was anastomosed 5 cm proximal to the ileocecal valve. Either arginine or glycine was administered to rats that underwent this surgery and rats with a normal small intestine. A total of four groups were prepared in this manner.2)Intestinal motility was analyzed using the Telemeter System. In order to record the motor function of the residual ileum, a 4-mm force transducer (FT) was sutured to the thin and fragile wall of the ileum 2 cm distal to the anastomotic site using 6-0 nylon, after intraperitoneal administration of Nembutal. Before suturing the FT, 0.1 mg/kg of atropine sulfate was admini … More stered subcutaneously. Using a small transmitter (for mice), fixed to the abdominal wall inside the abdominal cavity using 5-0 silk, contraction of the circular muscle was recorded repeatedly from 3 days after the embedding of the FT. At 3 and 6 weeks after 90% proximal small intestinal resection, the integral values for contraction waves before and after 12 or 24 hours of fasting were calculated every hour.3)Absorption function was assessed at 3 and 6 weeks after surgery by esophageally administering 50 mg of D-xylose and calculating the absorption rate based on urinary excretion using a 10-hour urine sample.[Results]1)For arginine, the integral values for hourly contraction waves after a meal was smaller than that before fasting ; however, for glycine, no difference between before and after fasting was observed. The number and duration of large contractile motions, that appeared during fasting and were thought to be equivalent to migrating myoelectric (or motor) complexes (MMC), showed no correlation to arginine administration.2)Absorption function was measured at 3 and 6 weeks after surgery. While arginine administration improved absorption at 3 and 4 weeks after surgery, no further improvement was seen at 6 weeks after surgery.[Conclusions]Arginine improved the absorption function of the short-small intestine model for only three weeks after surgery. Therefore, further investigations are required in order to ascertain the effects of arginine on intestinal motility. Less