Arginine effect on the gastrointestinal motility in the growing rat after massive small bowel resection

精氨酸对大肠小肠切除后生长大鼠胃肠动力的影响

• 批准号: 17591859
• 负责人: HEBIGUCHI Tatsuzo
• 金额: $ 1.79万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591859/
• 关键词: massive small intestinal resection; Arginine; gastrointestinal motility; telemeter system; 消化管運動; 吸収能; マイクロダイアリシス; 消化吸収

[Objective] Using our short-small intestine model that is suitable for investigating the importance of arginine in vivo, the motor functions of the stomach, duodenum, jejunum, and ileum were recorded without any restraint, and the results were compared to those of control rats.[Subjects and Methods] 1) Using 4-week-o1d male rat, 90% proximal small bowel resection (SBR) was performed, and in the control group, the intestine was cut and then anastomosed at 5 cm oral to the ileocecal valve. Either Elental-P with arginine or glycine was administered to rats that underwent this surgery and rats with a normal length of smal1 intestine. A total of four groups were prepared in this manner. 2) On the third week after surgery, the abdomen was reopened and a force transducer (FT) was sutured to the serous membrane of the pyloric portion of the stomach, the duodenum 3 cm anal to the pyloric ring, the jejunum 3 cm ana1 from Treitz ligament, and the ileum 2 cm anal to the anastomosis. From three day … More s later, gastrointestinal motility was recorded using a multichannel telemeter system (Star Medical Co.). 3) After fasting for 12 to 24 hours, gastrointestinal motility was repeatedly recorded and analyzed for 1-3 days. A single test cycle consisted of 12 hours of feeding and 12 hours of fasting. After the end of the test, the rats were sacrificed to ensure that the FTs were positioned properly.[Results] 1) For the control groups, immediately before feeding, migrating motility complexes(MMC) lasting 3-5 minutes were seen every 3-5 minutes in the duodenum, and then MMC lasting 3-4 minutes were seen in the jejunum. MMC disappeared with the start of feeding. However, MMC appeared 3-4 hours later and persisted at low-frequency. MMC increased gradually from 12 hours after the start of feeding and their frequency changed to every 3-5 minutes after 18 hours. The same MMC patterns were repeated with every administration. 2) For the SBR groups, immediately before feeding, MMC lasting 3-5 minutes were seen in the duodenum, but these disappeared with the start of feeding. Three to four hours later, MMC were again seen every 1-2 hours and MMC lasting 2-3 minutes were observed every 8-10 minutes from 18 hours later.MMC that occurred at the duodenum did not transmit well to the remaining ileum. 3) For both control and SBR groups, clear transmission of MMC to the ileum over the anastomosis was not seen.[Conclusion] Based on the current observations the housekeeping role of MMC in the residua1 ileum appears to be suppressed after SBR. There was no arginine effect on the gastrointestina1 motility in our model. Less

[目的]利用我们建立的适合于研究精氨酸在体内重要性的短小肠模型，在无任何约束的情况下记录胃、十二指肠、空肠和回肠的运动功能，并与对照组进行比较。[对象和方法] 1）4周龄雄性大鼠，行90%近端小肠切除术（SBR），对照组行小肠切除，并在离回盲瓣口5cm处缝合。对接受该手术的大鼠和小肠长度正常的大鼠给予含精氨酸或甘氨酸的Elental-P。以这种方式总共制备了四组。2)术后第3周，再次开腹，将力传感器（FT）缝合到胃幽门部分的浆膜，十二指肠距幽门环3cm，空肠距Treitz韧带3cm，回肠距吻合口2cm。从三天开始 ...更多信息 s后，使用多通道遥测系统（星星医疗公司）记录胃肠运动。3)禁食12 ~ 24小时后，重复记录胃肠动力并分析1-3天。一个测试周期包括12小时的喂养和12小时的禁食。试验结束后，处死大鼠以确保FT正确定位。[结果] 1）对照组在饲喂前，十二指肠每隔3- 5 min出现一次持续3- 5 min的移行运动复合物（MMC），空肠出现一次持续3-4 min的MMC。MMC随着进食开始而消失。MMC出现时间晚于3-4小时，持续低频。MMC从喂食后12小时开始逐渐增加，18小时后其频率变为每3-5分钟一次。每次给药均重复相同的MMC模式。2)对于SBR组，在喂食前即刻，在十二指肠中观察到持续3-5分钟的MMC，但这些MMC随着喂食开始而消失。3 ~ 4 h后，每隔1-2 h再次出现一次MMC，18 h后每隔8-10 min出现一次持续2-3 min的MMC，发生于十二指肠的MMC不能很好地传递到其余回肠。3)对于对照组和SBR组，均未观察到MMC通过吻合口明显传输至回肠。[结论] SBR后MMC在残余回肠中的管家作用受到抑制。在我们的模型中，精氨酸对胃肠道运动没有影响。少

项目成果

消化吸収機能からみたHirschsprung病類縁疾患の栄養管理-広範囲腸管神経節細胞減少症の1例を経験して-

从消化吸收功能角度进行先天性巨结肠相关疾病的营养管理-一例广泛性肠神经节血细胞减少症的经验-

• 发表时间: 2006
• 期刊: 小児外科 36
• 作者: Harada C;Okumura A;Namekata K;Nakamura K;Mitamura Y;Ohguro H;Harada T;Mitamura Y;Harada C;蛇口 達造
• 通讯作者: 蛇口 達造

Nutritional management of disseminated Hypoganglionosis, a pseudo-Hirschsprung's disease, from the perspective of intestinal absorption capacity : A case report

从肠道吸收能力的角度对播散性神经节减退症（一种假性先天性巨结肠）进行营养管理：一例报告

• 发表时间: 2006
• 期刊: Jpn J Pediatr Surg 38(6)
• 作者: Hebiguchi Tatsuzo;et. al.
• 通讯作者: et. al.

アルギニン代謝からみた短腸症候群の栄養管理

从精氨酸代谢角度对短肠综合征进行营养管理

• 发表时间: 2006
• 期刊: 小児外科 38
• 作者: Harada C;Okumura A;Namekata K;Nakamura K;Mitamura Y;Ohguro H;Harada T;Mitamura Y;Harada C;蛇口 達造;蛇口 達造
• 通讯作者: 蛇口 達造

Progress and a limit in the pediatric home parenteral nutrition-From a viewpoint of catheter sepsis in the patients used Broviac catheter-

儿科家庭肠外营养的进展与局限性-从使用Broviac导管的患者导管败血症的角度-

• 发表时间: 2006
• 期刊: Nutritional assessment and Therapy(in Japanese) 23(3)

小児における在宅静脈栄養法の進歩と限界-Broviac型カテーテル使用によるカテーテル敗血症の検討から-

儿童家庭肠外营养的进展和局限性 - 来自使用 Broviac 型导管引起的导管败血症的研究 -

• 发表时间: 2006
• 期刊: 栄養評価と治療 23
• 作者: Harada C;Okumura A;Namekata K;Nakamura K;Mitamura Y;Ohguro H;Harada T;Mitamura Y;Harada C;蛇口 達造;蛇口 達造;蛇口 達造
• 通讯作者: 蛇口 達造