Arginine effect on the gastrointestinal motility in the growing rat after massive small bowel resection

[目的]采用适合于研究精氨酸在体内重要性的短肠模型，不受束缚地记录胃、十二指肠、空肠和回肠的运动功能，并将结果与对照组进行比较。[对象和方法]1)4周龄雄性大鼠，行90%近端小肠切除(SBR)，对照组切断肠，在回盲瓣口5 cm处吻合。将Elental-P与精氨酸或甘氨酸一起应用于接受该手术的大鼠和肠道长度正常的大鼠。以这种方式共准备了四个小组。2)术后第3周，再次开腹，将力传感器(FT)缝合于胃幽门部分浆膜、十二指肠距幽门环3 cm、空肠距Treitz韧带3 cm、回肠距吻合口2 cm。从三天…开始更多的S之后，用多通道遥测系统(Star Medical Co.)记录胃肠运动。3)禁食12~24小时后，重复记录和分析胃肠运动1~3天。一个测试周期包括12小时的进食和12小时的禁食。[结果]1)对照组在饲养前，十二指肠每隔3~5min可见持续3~5min的移行运动复合体(MMC)，随后空肠可见持续3~4min的MMC。随着进食的开始，MMC消失了。而MMC则在3-4小时后出现，并维持在低频率。MMC自投喂后12h开始逐渐增加，至18h后频率每隔3~5min变化一次。每次给药都重复相同的MMC模式。2)SBR组饲喂前，十二指肠内可见持续3-5分钟的MMC，但随着饲喂开始，MMC消失。3~4小时后，每隔1~2小时可见MMC，18小时后每8~10分钟可见持续2~3分钟的MMC，发生在十二指肠的MMC向其余回肠传导不畅。3)对照组和SBR组均未见MMC通过吻合口向回肠明显传递。[结论]根据目前的观察结果，SBR后MMC在残留回肠中的管家作用似乎受到抑制。在我们的模型中，精氨酸对胃肠运动没有影响。较少

[Objective] Using our short-small intestine model that is suitable for investigating the importance of arginine in vivo, the motor functions of the stomach, duodenum, jejunum, and ileum were recorded without any restraint, and the results were compared to those of control rats.[Subjects and Methods] 1) Using 4-week-o1d male rat, 90% proximal small bowel resection (SBR) was performed, and in the control group, the intestine was cut and then anastomosed at 5 cm oral to the ileocecal valve. Either Elental-P with arginine or glycine was administered to rats that underwent this surgery and rats with a normal length of smal1 intestine. A total of four groups were prepared in this manner. 2) On the third week after surgery, the abdomen was reopened and a force transducer (FT) was sutured to the serous membrane of the pyloric portion of the stomach, the duodenum 3 cm anal to the pyloric ring, the jejunum 3 cm ana1 from Treitz ligament, and the ileum 2 cm anal to the anastomosis. From three day … More s later, gastrointestinal motility was recorded using a multichannel telemeter system (Star Medical Co.). 3) After fasting for 12 to 24 hours, gastrointestinal motility was repeatedly recorded and analyzed for 1-3 days. A single test cycle consisted of 12 hours of feeding and 12 hours of fasting. After the end of the test, the rats were sacrificed to ensure that the FTs were positioned properly.[Results] 1) For the control groups, immediately before feeding, migrating motility complexes(MMC) lasting 3-5 minutes were seen every 3-5 minutes in the duodenum, and then MMC lasting 3-4 minutes were seen in the jejunum. MMC disappeared with the start of feeding. However, MMC appeared 3-4 hours later and persisted at low-frequency. MMC increased gradually from 12 hours after the start of feeding and their frequency changed to every 3-5 minutes after 18 hours. The same MMC patterns were repeated with every administration. 2) For the SBR groups, immediately before feeding, MMC lasting 3-5 minutes were seen in the duodenum, but these disappeared with the start of feeding. Three to four hours later, MMC were again seen every 1-2 hours and MMC lasting 2-3 minutes were observed every 8-10 minutes from 18 hours later.MMC that occurred at the duodenum did not transmit well to the remaining ileum. 3) For both control and SBR groups, clear transmission of MMC to the ileum over the anastomosis was not seen.[Conclusion] Based on the current observations the housekeeping role of MMC in the residua1 ileum appears to be suppressed after SBR. There was no arginine effect on the gastrointestina1 motility in our model. Less