The effect of arginine supplementation on intestinal growth and absorption after massive small bowel resection in growing rats

补充精氨酸对生长大鼠大段小肠切除术后肠道生长和吸收的影响

• 批准号: 08457471
• 负责人: HEBIGUCHI Tatsuzo
• 金额: $ 4.86万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457471/
• 关键词: massive small bowel resection; arginine; growth factor; growth hormone; intestinal growth; microdialysis; tubulointerstitial fibrosis; 腸管の成長; 吸収能

*Purpose* We studied effects of arginine (Arg) supplementation on intestinal growth and absorption after massive small bowel resection in growing rats.*Material and Methods* Four-week-old male Sprague-Dawley rats underwent a 90% small bowel resection. From the fourth day after surgery, they were divided into group 1 and 2, and pair-fed by elemental diets (0.8 kcal/ml, 50 ml/day) withL-arginine or L-glycine as an isonitrogenous and isoenergetic supplement for 3 and 6weeks. They were compared with each other at 3 and 6 weeks after surgery. A statistical analysis was performed using the unpaired Student's test and the one-way factorial analysis of variance (ANOVA) using Bonferroni/Dunn multiple comparison test. AP-value of <.05 was considered significant.*Results* There were no significant differeces between the two groups in food intake, body weight, tail length, remaining ileal length, and plasma somatomedin C/IGF-I level at 3 weeks after surgery. However, the mean height of ileal villi at 3 weeks and remaining ileal length at 6 weeks in group 1 showed significantly higher and longer than those in group 2, respectively. Growth hormone-releasing hormone provocative tests (1 mug per rat, intravenously) showed the more significant elevation of growth hormone (GH) secretion in the Arg supplement group than that of glycine (Gly) supplement group at 5 minutes. There were no significant differences between basal levels of plasma rat GH in both groups. Plasma Cit level is changed by Arg supplementation, and Arg level is changed by massive small bowel resection and Arg supplementation at 3 weeks. However, there were no differnces of plasma levels of Cit and Arg between the two groups at 6 weeks. Histological study of the kidney showed tubulointerstitial fibrosis in group 2 at 6 weeks after surgery.*Conclusion* It is suggested that Arg has a possible significant role of GH secretion, intestinal growth and improving absorptive capacity after massive small bowel resection.

* 目的 * 我们研究了精氨酸（Arg）补充对生长期大鼠小肠切除术后肠道生长和吸收的影响。*材料和方法 * 4周龄雄性Sprague-Dawley大鼠进行90%小肠切除术。术后第4天起分为1、2组，分别给予基础饲料（0.8kcal/ml，50 ml/d）和L-精氨酸或L-甘氨酸作为等氮等能补充剂，喂养3、6周。分别于术后3周和6周进行比较。使用非配对Student检验和使用Bonferroni/Dunn多重比较检验的单因素方差分析（ANOVA）进行统计分析。<.05的AP值被认为是显著的。结果术后3周，两组大鼠的摄食量、体重、尾长、剩余回肠长度及血浆生长调节素C/IGF-I水平均无显著性差异。第1组3周回肠绒毛高度和第6周回肠剩余长度均显著高于第2组。生长激素释放激素激发试验（1 μ g/只大鼠，静脉注射）显示，在5分钟时，精氨酸补充组的生长激素（GH）分泌比甘氨酸（Gly）补充组显著升高。两组大鼠血浆GH基础水平无显著差异。血浆Cit水平通过补充Arg而改变，并且Arg水平在3周时通过大量小肠切除和补充Arg而改变。6周时两组血浆Cit、Arg水平无明显差异。肾脏组织学研究显示，第2组在术后6周出现肾小管间质纤维化。结论精氨酸对小肠大部切除术后生长激素的分泌、小肠的生长发育及吸收功能的改善有重要作用。

项目成果

蛇口達造: "銅補充非反応性顆粒球減少症と低銅血症の長期静脈栄養例" 小児外科. 28・10. 1223-1229 (1996)

Tatsuzo Fukou：“长期肠外营养伴无反应性粒细胞减少症和低铜血症的病例”小儿外科28・10（1996）。

蛇口達造: "小児期短腸症侯群の栄養管理と長期予後" JJPEN. 19・10. 949-949 (1997)

Tatsuzo Fukou：“短肠综合征儿童的营养管理和长期预后”JJPEN 19・10（1997）。

HEBIGUCHI T.et al.: "Treatment of short gut syndrome using growth factors---Intestinal adaptation and growth factor" IGAKU-NO-AYUMI. 181. 504-505 (1997)

HEBIGUCHI T.et al.：“使用生长因子治疗短肠综合征---肠适应和生长因子” IGAKU-NO-AYUMI。

蛇口達造: "成長因子を利用した短腸症候群の治療:消化管代償能と成長因子" 医学のあゆみ. 181・04(掲載ページ未定). (1997)

Tatsuzo Takanashi：“使用生长因子治疗短肠综合征：胃肠代偿能力和生长因子”医学史181/04（页数尚未确定）。

Hebiguchi T, et al: "The effect of arglnine supplementation on growth hormone reiease and Intestinal mucosal growth after masslve small bowel resection in growing rats" J Pediatr Surg. 32. 1149-1153 (1997)

Hebiguchi T 等人：“补充精氨酸对生长大鼠大规模小肠切除后生长激素释放和肠粘膜生长的影响”J Pediatr Surg。