Role of DEC1 and MTf in osteogenic and chondrogenic differentiation of mesenchymal stem cells

点击翻译按钮获取中文摘要

Since MSC has multilineage potential, we investigated the roles of DEC1 in osteogenic and adipogenic differentiation of human bone marrow-derived MSC. After osteogenic induction of MSC in medium containing dexamethasone, beta-glycerophosphate, and ascorbic acid, DEC1 expression gradually increased from day 5 to day 14 ; while expression levels of DEC1 mRNA markedly decreased on days 3 and 7 after adipogenic induction. Under osteogenic conditions, infection with DEC1-expressing adenovirus enhanced the induction of osteopontin and alkaline phosphatase, and matrix calcification. Even in the absence of the osteogenic induction medium, DEC1 overexpression increased osteopontin and alkaline phosphatase expression, although it did not induce calcification. In contrast, forced expression of DEC1 suppressed adipogenic differentiation of MSC. These findings indicate that increased DEC1 expression facilitates osteogenic differentiation of mesenchymal stem cells, and inhibits the onset of adipogenic differentiation.