Role of novel protein (p28^<nrp>) induced by ionizing radiation

电离辐射诱导的新型蛋白质 (p28^<nrp>) 的作用

• 批准号: 15591999
• 负责人: HOTOKEZAKA Yuka
• 金额: $ 2.24万
• 依托单位: NAGASAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591999/
• 关键词: p28^<nrp>; ionizing radiation; repair DNA; apoptosis; p54^<nrb>

It is well known that the cell arrest its cell cycle in order to repair damaged DNA in response to ionizing radiation. However, the cell may activate one or more of the apoptosis pathway to eliminate cells harboring severely damaged DNA. Here we report the identification of a new protein factor p28^<nrp> (28 kDa) that was induced in the nucleus in response to ionizing radiation. Westernblotting using p54^<nrb> peptide antibody demonstrated that the p28^<nrp> was an N-terminal part of the p54^<nrb> (nuclear RNA-binding protein). We showed that the p54^<nrb> was cleaved and an N-terminal portion (p28^<nrp>) entered into nucleus in response to ionizing radiation. It is shown recently that the polypyrimidine tract-binding protein-associated splicing factor (PSF)・p54^<nrb> complex cooperates with Ku protein to reconstitutes efficient double-strand break rejoining (J.Biol Chem,2005). Thus, it is suggested that the p28^<nrp> plays a role in repair or apoptosis to ionizing radiation.

众所周知，细胞停止其细胞周期，以修复受损的DNA，以响应电离辐射。然而，细胞可以激活一种或多种凋亡途径以消除携带严重受损DNA的细胞。在这里，我们报告了一个新的蛋白质因子p28^<nrp>（28 kDa），诱导细胞核中的电离辐射响应的鉴定。用p54^肽抗体进行的Westernblotting<nrb>证明p28^<nrp>是p54^<nrb>（核RNA结合蛋白）的N端部分。我们发现，在电离辐射的作用下，p54^<nrb>被切割，N末端部分（p28^<nrp>）进入细胞核。最近的研究表明，多聚嘧啶片段结合蛋白相关剪接因子（polypyrimidine tract-binding protein-associated splicing factor，PSF）·p54^<nrb>复合物与Ku蛋白协同作用，重建有效的双链断裂重接（J.Biol Chem，2005）。因此，提示p28 α<nrp>在电离辐射的修复或凋亡中起作用。