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Expression and characterization of vanilloid receptor subtype 1 in human dental pulp cell cultures.

人牙髓细胞培养物中香草酸受体亚型 1 的表达和表征。

基本信息

批准号：
15592024
负责人：
TOKUDA Masayuki
金额：
$ 2.24万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592024/
关键词：
Dental pulp cells Temperature-sensitive receptor VR-1 Inflammatory cytokines TNF-α IL-6 MAPK apoptosis TNF-a

项目摘要

We examined whether human dental pulp fibroblasts(PF-10) have vanilloid receptor subtype 1(VR1) and its relationship with interleukin(IL)-6 expressions. Results of RT-PCR, Western blotting and immunocytochemical analysis indicate VR1 expression on PF-10 cells. As shown by ELISA, VR1 antagonist, capsazepine inhibited capsaicin-induced IL-6 production which is implicated in pulp inflammation. Furthermore, we investigated the activities of mitogen-activated protein kinases(MAPKs) in capsaicin induced IL-6 production. The phosphorylation of p38 MAPK and c-Jun NH_2-terminal kinase(JNK) were detected after capsaicin stimulation. p38 MAPK is involved in capsaicin-induced IL-6 production, as shown by the use of specific inhibitors of this kinase. The result of EMSA showed capsaicin inhibited tumor necrosis factor-alpha(TNF-α)-induced nuclear factor-kappa B(NF-κB) activations. These results suggest that the activation of VR1 may play an important role in dental pulp inflammation.

本研究旨在探讨人牙髓成纤维细胞（PF-10）中是否存在香草酸受体亚型1（VR 1）及其与白细胞介素（IL）-6表达的关系。RT-PCR、Western blotting和免疫细胞化学分析结果显示PF-10细胞表达VR 1。如ELISA所示，VR 1拮抗剂辣椒平抑制辣椒素诱导的IL-6产生，这与牙髓炎症有关。此外，我们还研究了辣椒素诱导IL-6产生过程中丝裂原活化蛋白激酶（MAPK）的活性。辣椒素刺激后，检测p38 MAPK和c-Jun氨基末端激酶（JNK）的磷酸化。p38 MAPK参与辣椒素诱导的IL-6产生，如该激酶的特异性抑制剂的使用所示。EMSA结果显示辣椒素抑制肿瘤坏死因子-α（TNF-α）诱导的核因子-κ B（NF-κB）活化。提示VR 1的激活可能在牙髓炎症中起重要作用。