The role and expression mechanism of an inflammatory mediator in human denial pulp cell

人牙髓细胞炎症介质的作用及表达机制

• 批准号: 15592034
• 负责人: HASHIZUME Hideki
• 金额: $ 2.11万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592034/
• 关键词: human dental pulp cell; Plasminogen activator; IL-1β; protein tyrosin kinase; Plasminogen Activator; チロシンリン酸化; NFκB; Plasminogen activator; IL-1

1.IL-1β(50pg/ml) activated t-PA in the lysate and medium in a time-dependent (0〜12hrs) manner.2.IL-1β activated t-PA in the lysate and medium in a concentration -dependent(50〜100pg) manner.3.Pyrrolidine dithocarbamate, a NFκB inhibitor inhibited the IL-1β-induced tPA activation.4.Genistein and herbimycin, protein tyrosine kinase inhibitors, inhibited the IL-1β-induced tPA activation.5.On the contrary, orthovanadate, a protein tyrosine phosphatase inhibitor, augmented the effect of IL-1β.These observations suggest that regulation of tyrosine phosphorylation is necessary for IL-1β induced t-PA activation, and the synthesis of t-PA via NFκB is involved in the effect of IL-1β in HDP.6.Phorbol 12-myrisatate 13-acetate, protein kinase C activator t PA augmented the effect if IL-1β induced tPA activation.7.Ionomycine, Ca^<2+> mobilizing reagent did not augment the IL-1β induced tPA activation

1.IL-1β(50pg/ml)以时间依赖的方式(0~12小时)激活裂解液和培养液中的t-PA。2.IL-1β以浓度依赖的方式(50~100pg)激活裂解液和培养液中的t-PA。3.核因子κB抑制剂吡咯烷二硫代氨基甲酸酯抑制IL-1β诱导的tPA激活。4.蛋白酪氨酸激酶抑制剂Genistein和赫比霉素抑制IL-1β诱导的tPA激活。5.蛋白酪氨酸磷酸酶抑制剂正钒酸增强IL-1β的作用。提示IL-1β诱导t-PA活化需要调节酪氨酸磷酸化，而通过核因子κB合成t-PA参与了IL-1β的作用。6.12-肉豆蔻酸13-乙酸酯、蛋白激酶C激动剂t-PA可增强IL-1β诱导t-PA活化的作用。激动剂不能增强IL-1β诱导的tPA活性