Nano area evaluation for adhesive mechanism of resin to dentin

本研究的目的是解决树脂与牙本质的粘接机理。采用纳米硬度计对树脂-牙本质界面的力学性能进行了评价，并对树脂-牙本质界面进行了微拉伸粘结强度测试和用氩离子蚀刻技术对树脂-牙本质界面进行了FE-SEM观察。本研究对市面上已有的自酸蚀系统进行了评价，用ENT-1100a纳米压痕测试仪测量了树脂-牙本质界面的粘结层。从纳米硬度的结果来看，本研究中评估的商用自酸蚀粘结剂在粘接层的硬度方面没有显著差异。通过微拉伸粘结强度测试来评估一种可用的自酸蚀系统的人牙本质粘结强度。两步自酸蚀系统的微拉伸结合强度高于一步自酸蚀系统和瓶装自酸蚀系统。我们找不到Nanoh…与采用场发射扫描电子显微镜(FE-SEM)结合Ar-离子刻蚀技术，观察了自酸蚀粘结剂与牙本质的超微形态相互作用。Ar-离子束刻蚀技术被广泛应用于树脂-牙本质界面的扫描电子显微镜观察，将混合层描述为由网状结构组成。用有限元扫描电子显微镜观察了Ar离子酸蚀后的网络结构，并观察了自酸蚀粘结剂的树脂-牙本质界面。结果表明，Ar离子酸蚀树脂-牙本质界面的网状结构是由多孔化底漆和粘结树脂、杂化层和部分暴露的胶原纤维组成的。Ar离子酸蚀30s~2步自酸蚀粘结剂的杂化层网络宽度(2~5μm)与文献报道相近，而一瓶自粘结剂在30s的Ar离子酸蚀后，牙本质与粘接剂之间的网络宽度变窄或没有网络宽度(0~0.5μm)，这表明杂化层的宽度不是牙本质粘结所必需的，牙本质粘接不仅仅是对牙本质的机械互锁。较少

The purpose of this study was to solve the adhesive mechanism of resin to dentin. The mechanical property of resin-dentin interface was evaluated by nanohardhess mesurements, and microtensile bond strength test to human dentin and FE-SEM observations of resin-dentin interface with argon ion etch technique were also performed. The self-etch systems which available on the market were evaluated in this study.We measured adhesive layer of resin-dentin interface by self-etch systems using an ENT-1100a Nanoindentation tester (Elionix, Japan). From the nanohardness results, commercially available self-etch adhesives evaluated in this study showed no significant difference in, hardness of adhesive layer.The human dentin bond strength of an available self-etch systems were evaluated by microtensile bond strength tests. The microtensile bond strength of 2-step self-etch systems were higher than 1-step or one bottled self-etch systems. We were not able to find about the relationship between nanoh … More ardness and bond strength of self-etch systems.The ultra-morphological interaction to human dentin of self-etch adhesive was observed by field-emission SEM (FE-SEM) with argon-ion (Ar-ion) beam etch technique. Ar-ion beam etch technique have been widely employed as SEM observation of resin-dentin interface, describing hybrid layer as composed of a meshwork structure. We examine the meshwork structure after ar-ion etch by FE-SEM, and also observed resin-dentin interface of self-etch adhesives. Our data suggested the meshwork strucrure on resin-dentin interface by ar-ion etch is composed of porymerized primer and bonding resin, hybrid layer and partially exposed collagen fibers.Ar-ion etching of 30-sec's to 2 step self-etch adhesives demonstrated a wide hybrid layer meshwork width (2 to 5 μm) similar to that previously reported, while one-bottled self adhesives showed narrow or no meshwork width (0 tO 0.5 μm) between dentin and adhesive after 30-sec's Ar-ion etching, It was suggested that width of hybrid layer is not necessary for dentin adhesion, and dentin adhesion is not formed only mechanical interlocking to dentin. Less