New strategy for developing surface-treated functional titanium based on nano-structure and electronic state analyses

1.采用透射电镜研究了金属结合瓷与溅射镀金钛的结合增强机理。镀金后，钛侧缺陷层消失。如果没有金涂层，则认为钛原子通过熔接界面处的反应层向瓷侧扩散。另一方面，金对钛有很强的亲和力，因此金层被认为阻碍了钛向瓷内扩散和氧向钛内扩散。电子能量损失谱（EELS）的结果很好地解释了熔合界面结构和晶体结构。离子镀是钛表面处理的一种方法。采用电子束熔化和氩射频等离子体电离钛。离子镀钛膜的厚度约为100 nm，膜中的晶粒尺寸小于10 nm左右。进行横断面透射电镜观察。在直径约1毫米的丙烯酸树脂棒上镀上钛，并制作钛植入物的原型用于动物试验。这个植入物的好处是我们可以用显微切片机把钛植入物和周围的组织切片。将试验植入物置于小鼠左股骨骨干中段。术后1 ~ 4 d行纤维连接蛋白（FN）抗体免疫组化。结果提示血浆FN调节骨形成。

1.Binding enhancement mechanism of the metal bonded porcelain with the Au sputter-coated titanium was investigated using transmission election microscopy. The deficient layer on the titanium side disappeared by the Au sputter coating. Without the Au coating, titanium atoms is considered to diffuse on the porcelain side through the reaction layer at the fused interface. On the other hand, gold has a strong affinity for titanium, so the gold layer is considered to hinder titanium diffusing into the porcelain and oxygen diffusing into the titanium.2.The results of the electron energy loss spectroscopy (EELS) well explain the fused interface structure and the crystal structure.3.Ion plating is applied to one of titanium surface treatment methods. Ionized titanium was introduced by an electron beam melting and Ar RF plasma. The thickness of the ion-plated titanium film was about 100 nm and the crystal grain size in the film was around less than 10 nm. Cross-sectional TEM observation was performed.4.Acrylic resin rods at about 1 mm in diameter were subjected to the Ti ion plating and we prototyped titanium implants for animal trials. The benefit of this implant is that we've been able to slice the titanium implant with surrounding tissue using microtome. The trial implant was placed into the middle portion of the diaphysis of the left femur in mouse. After 1-4 days operation, immunohistochemistry with fibronectin (FN) antibody was performed. The results suggest that Plasma FN regulates bone formation.