New strategy for developing surface-treated functional titanium based on nano-structure and electronic state analyses

1.用透射电子显微镜研究了金属粘结瓷与镀金涂层钛的结合增强机理。Au溅射涂层使钛一侧的缺陷层消失。在没有Au涂层的情况下，钛原子被认为是通过熔合界面的反应层在瓷器一侧扩散的。另一方面，金与钛有很强的亲和力，因此金层被认为阻碍了钛扩散到瓷中，阻碍了氧扩散到钛中。2.电子能量损失谱(EELS)的结果很好地解释了熔融界面结构和晶体结构。3.离子镀被应用于钛表面处理方法之一。通过电子束熔炼和Ar射频等离子体引入离子化钛。离子镀钛膜的厚度约为100 nm，薄膜中的晶粒尺寸小于10 nm。对直径约1 mm的丙烯酸树脂棒进行钛离子镀处理，制作钛种植体模型，进行动物实验。这种植入物的好处是我们能够用切片机将钛植入物及其周围组织切片。在小鼠左侧股骨骨干中段放置试验性植入物。术后1~4d行纤维连接蛋白(FN)免疫组织化学染色。结果提示，血浆FN对骨形成有调节作用。

1.Binding enhancement mechanism of the metal bonded porcelain with the Au sputter-coated titanium was investigated using transmission election microscopy. The deficient layer on the titanium side disappeared by the Au sputter coating. Without the Au coating, titanium atoms is considered to diffuse on the porcelain side through the reaction layer at the fused interface. On the other hand, gold has a strong affinity for titanium, so the gold layer is considered to hinder titanium diffusing into the porcelain and oxygen diffusing into the titanium.2.The results of the electron energy loss spectroscopy (EELS) well explain the fused interface structure and the crystal structure.3.Ion plating is applied to one of titanium surface treatment methods. Ionized titanium was introduced by an electron beam melting and Ar RF plasma. The thickness of the ion-plated titanium film was about 100 nm and the crystal grain size in the film was around less than 10 nm. Cross-sectional TEM observation was performed.4.Acrylic resin rods at about 1 mm in diameter were subjected to the Ti ion plating and we prototyped titanium implants for animal trials. The benefit of this implant is that we've been able to slice the titanium implant with surrounding tissue using microtome. The trial implant was placed into the middle portion of the diaphysis of the left femur in mouse. After 1-4 days operation, immunohistochemistry with fibronectin (FN) antibody was performed. The results suggest that Plasma FN regulates bone formation.