The developmentof artificial tympanic membrane by tissue engineering for reproduction and pathologic elucidation of middle ear cholesteatoma.

通过组织工程开发人工鼓膜用于中耳胆脂瘤的繁殖和病理学阐明。

• 批准号: 20591993
• 负责人: TANAKA Yasuhiro
• 金额: $ 2.83万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591993/
• 关键词: 再生医学; 細胞・組織

We developed artificial middle ear mucosal sheets from cultured middle ear mucosa that was taken from middle ear bulla of a white rabbit by using the temperature-responsive culture dish. And also, we developed tympanic membrane epithelium sheets consist of extra cellular matrix and keratinocyte that was taken from external ear canal of the same rabbit. After that, we finally manufactured an artificial tympanic membrane by fusion of artificial middle ear mucosal sheets and tympanic membrane epithelium sheets. We confirmed this artificial tympanic membrane was resembled normal tympanic membrane from the viewpoint of pathohistologic characteristics by immunohistochemical staining and electron microscope findings. Although, we attempted to develop an experimental middle ear cholesteatoma model by using the artificial tympanic membrane, it was impossible to develop it only by the stimulation of some chemical mediators and cytokines.On the other hand, we confirmed the suppression of bone and granulation tissue hyperplasia in the middle ear bulla after implantation of artificial middle ear sheets.

本研究采用温度反应式培养皿，从白兔中耳大疱处提取中耳粘膜，制备了人工中耳粘膜片。同时，我们从同一只家兔的外耳道提取了由细胞外基质和角质形成细胞组成的鼓膜上皮片。最后，我们将人工中耳粘膜片与鼓膜上皮片融合制成人工鼓膜。通过免疫组化染色和电镜观察，证实人工鼓膜在病理组织学上与正常鼓膜相似。虽然我们尝试用人工鼓膜建立实验性中耳胆脂瘤模型，但仅靠一些化学介质和细胞因子的刺激是不可能建立的。另一方面，我们证实了人工中耳片植入后中耳球骨和肉芽组织增生受到抑制。

项目成果

真珠腫とは

什么是胆脂瘤？

• 发表时间: 2008
• 期刊: 日本医事新報 4391
• 作者: Matsumoto S;Hato N;Kohno H;Yamada Y;Kisaki H;Wakisaka H;Takahashi H;Honda N;Gyo K;鈴木幹男;田中康広
• 通讯作者: 田中康広

Middle ear mucosa regeneration by grafting of three-dimensional middle ear mucosal organ

三维中耳粘膜器官移植促进中耳粘膜再生

• 发表时间: 2008
• 期刊: Surgery of the ear current topics
• 作者: Sawai N;Hato N;Teraoka M;Takahashi H;Wakisaka H;Gyo K;鈴木幹男;脇坂浩之;田中康広
• 通讯作者: 田中康広

中耳真珠腫

中耳胆脂瘤

• 发表时间: 2009
• 期刊: 医学と薬学 62
• 作者: Hato N;Sawai N;Teraoka M;Wakisaka H;Takahashi H;Hinohira Y;Gyo K.;脇坂浩之;田中康広
• 通讯作者: 田中康広