Gene profiling in adenoid cystic carcinoma cells using in-house cDNA microaray

点击翻译按钮获取中文摘要

We created an in-house cDNA microarray containing 1,423 cDNA clones derived from an oral squamous cell carcinoma(SCC) cDNA library and 778 cDNA clones derived from a salivary gland tumor(SGT) cDNA library. mRNAs obtained from adenoid cystic carcinoma(ACC) cells that originated from SGT were analyzed using this microarray system. Additionally, further more microarray assay was performed with Affymetrix GeneChip^<TM>, which attached more than 38,000 genes, in order to examine more genes. Microarray detected up-regulated expression of many genes, which expressed twice or more in ACC than in normal salivary gland tissue. Representative up-regulated genes detected were regulator of Fas-induced apoptosis, tumor necrosis factor superfamily, maspin, annexin A8,fibroblast growth factor receptor 1,insulin-like growth factor 2,fibrin2,amyloid beta 4,collagen, and laminin. On the other hand, representative down-regulated genes consisted of WT1,BCL-2,Inhibrin, FAT tumor suppressor homolog, Notch, S100 calcium binding protein A1,chemokine, tumor necrosis factor superfamily, stathmin, CD44,and lysozyme. Of the up-regulated genes, maspin and stathmin were selected for further analyses including Western blotting and immmunohistochemical staining. We found higher expression of maspin in SGT compared with oral sSCC, indicating that maspin is a molecular marker specific to ACC. Interestingly, stathmin mRNA and protein expression levels were significantly higher in both ACC and SCC, indicating that stathmin is a molecular marker specific to malignancy.