Isolation of a novel tumor suppressor gene on the Chromosome 11 associated with oral squamous cell carcinoma.

为了寻找11号染色体上与口腔鳞状细胞癌（SCC）相关的肿瘤抑制基因的存在，我们使用22个微卫星标记对31例口腔鳞状细胞癌患者进行了高分辨率缺失定位。我们的数据显示了两个不同的，经常被删除的区域。第一个杂合性缺失（LOH）频繁的区域限制在染色体11q23上相隔3厘米的标记D11S939和D11S924之间。第二个共同缺失区域位于标记D11S912和D11S910之间，在11q25处相隔7 cM。我们的研究结果表明，在11号染色体的长臂上存在至少2个参与口腔鳞状细胞癌发展的肿瘤抑制基因。为了确定11q23和11q25染色体上的候选肿瘤抑制基因，采用差异显示法和代表性差异分析，在这些位点上使用LOH和不LOH的肿瘤。分离到4个DNA片段，其中1个片段位于11q染色体上。DNA片段的序列被检测到，它被认为是一个推定的肿瘤抑制基因的一部分，参与了口腔鳞状细胞癌的发生和发展。

To search for the existence of tumor-suppressor gene(s) associated with oral squamous cell carcinoma (SCC) on chromosome 11, we have performed high-resolution deletion mapping in 31 patients with oral SCC using 22 microsatellite markers for this chromosomal region. Our data show 2 distinct, commonly deleted regions. The first region, with frequent loss of heterozygosity (LOH), was restricted between markers D11S939 and D11S924 separated by 3 centimorgans (cM) on chromosome 11q23. The second region of common deletion was identified between markers D11S912 and D11S910, separated by 7 cM at 11q25. Our results suggest tnat at least 2 tumor-suppressor genes involved in the development of oral SCC are present on the long arm of chromosome 11. To identify the candidate tumor suppressor genes at chromosome 11q23 and 11q25, differential display method and representational difference analysis, using tumors with and without LOH at these loci. Four DNA fragments were isolated and one of them was confirmed the presence on chromosome 11q. The sequence of the DNA fragment was detected and it was considered as a part of a putative tumor suppressor gene involved in the oncogenesis and development of oral squamous cell carcinoma.